Catalog # Aliquot Size U225-911-05 3 x 5 nmol U225-911-20 3 x 20 nmol U225-911-50 3 x 50 nmol UBE2J1 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # U225-911 Lot # Z2109-26 Specificity Formulation UBE2J1 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human UBE2J1 expression. shipped at room temperature. Product Description Reconstitution Protocol UBE2J1 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human UBE2J1 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is BC013973. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; 1x stock solutions for storage. UBC6; Ubc6p Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the UBE2E3 (UBCH9) Protein U219-30H lyophilized and resuspended siRNAs are stored at or UBE2F Protein U220-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2G1 (UBC7) Protein U221-30H µM can undergo up to 50 freeze-thaw cycles without UBE2H Protein U223-30H significant degradation. For long-term storage, it is UBE2I Protein U224-30H recommended that the siRNA is stored at -70oC. For most UBE2J1 Protein U225-30G favorable performance, avoid repeated handling and UBE2K (UBC1) Protein U227-30H multiple freeze/thaw cycles. UBE2L6 (UBCH8) Protein U229-30H Scientific Background UBE2J1 is a member of the E2 ubiquitin-conjugating UBE2J1 siRNA Set I siRNA duplexes targeted against three exon regions enzyme family which is located in the membrane of the endoplasmic reticulum (ER) and may contribute to Catalog # U225-911 quality control ER-associated degradation by the Specific Lot Number Z2109-26 ubiquitin-proteasome system. UBE2J1 is play an important Packaging Specifications 2.5 nmol/tube for 3 x 5nmol role in the homeostatic regulation of MHC class I assembly and expression (1). HRD1 and UBE2J1 associate Format Lyophilized powder Stability 1yr at -70oC from date of shipment in a complex together with non-2m bound MHC class I Storage & Shipping The lyophilized powder is stable for at heavy chains, Derlin 1, and p97 and discriminate least 4 weeks at room temperature. It is misfolded MHC class I from conformational MHC I-2m- recommended that the lyophilized peptide heterotrimers. and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can References undergo up to 50 freeze-thaw cycles without significant degradation. For 1. Burr ML. et. al: HRD1 and UBE2J1 target misfolded long-term storage, it is recommended MHC class I heavy chains for endoplasmic reticulum- that the siRNA is stored at -70oC. For associated degradation. Proc Natl Acad Sci U S A. most favorable performance, avoid repeated handling and multiple 2011 Feb 1. freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .