MONOCLONAL ANTI-BOVINE SERUM ALBUMIN (BSA) Clone BSA-33 Mouse Ascites Fluid Product No. B 2901 Product Description specificity may prove useful in the identification of bovine Monoclonal Anti-Bovine Serum Albumin (BSA) (mouse serum albumin. IgG2a isotype) is produced by the fusion of mouse myeloma cells and splenocytes from an immunized Reagents mouse. Bovine serum albumin was used as the The product is provided as a liquid with 0.1% sodium immunogen. The isotype is determined using Sigma azide as a preservative. ImmunoTypeÔ Kit (Product Code ISO-1) and by a double diffusion immunoassay using Mouse Monoclonal Anti- Precautions and Disclaimer body Isotyping Reagents (Product Code ISO-2). Due to the sodium azide content a material safety data sheet (MSDS) for this product has been sent to the Monoclonal Anti-BSA recognizes the 67 kDa band of attention of the safety officer of your institution. Consult SDS-denatured and reduced BSA using an the MSDS for information regarding hazards and safe immunoblotting technique. The antibody is specific for handling practices. bovine serum albumin and is highly cross reactive with goat and sheep serum albumins. The product is Storage/Stability somewhat less cross reactive with dog, turkey and horse For continuous use, store at 2-8 °C for up to one month. serum albumins. Monoclonal Anti-BSA does not For extended storage, solution may be frozen in working crossreact with human, rabbit, guinea pig, chicken, aliquots. Repeated freezing and thawing is not hamster, pig, mouse, cat, pigeon, rat or donkey serum recommended. If slight turbidity occurs upon prolonged albumins, or with chicken and turkey egg albumins. storage, clarify by centrifugation before use. Monoclonal Anti-Bovine Serum Albumin may be used for Product Profile determination and quantification of BSA by ELISA, A minimum working dilution of 1:1,000 was determined competitive ELISA and immunodot blot. The antibody by an ELISA using 10 mg/ml of BSA as the coating may be used for the immunoaffinity purification and solution. It has been noted that BSA is adsorbed most removal of BSA from various biological fluids such as cell effectively on polyvinyl microtiter plates. culture media and in vitro-produced monoclonal antibodies. In order to obtain best results, it is recommended that each individual user determine their working dilution by Bovine serum albumin is the major protein produced by titration assay. the liver and represents more than half of the total protein found in serum. BSA is found in many biological substances such as serum supplemented cell culture PCS/KMR 08/02 media and its products, in foods and forensic preparations. A monoclonal antibody of species Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. .