Microalgae strain catalogue A strain selection guide for microalgae users: cultivation and chemical characteristics for high added-value products Gonzalo M. Figueroa-Torres a, Elisabeth Bermejo-Padilla a. Jon K. Pittman b, Constantinos Theodoropoulos a a Department of Chemical Engineering and Analytical Science, Biochemical and Bioprocess Engineering Group b Department of Earth and Environmental Sciences The University of Manchester, Manchester, UK, M13 9PL 3rd Edition Page | 1 Microalgae strain catalogue - A strain selection guide for microalgae users 3rd edition, University of Manchester, Manchester,UK EnhanceMicroAlgae 2021 The 3rd edition of this catalogue contains information on the cultivation and composition characteristics of 37 microalgae. Each entry includes relevant links to Atlantic Area stakeholders known to have a relevant connection with each of the species listed, be it in the form of culture collections, research expertise, technology developers, or biomass producers. We invite the readers to visit and/or join the EnhanceMicroAlgae Stakeholder database: an easily accessible, visual and open access database that brings together all the European Atlantic Area players working in the microalgae sector. Contributing authors: Dr. Gonzalo M. Figueroa-Torres a, Dr. Elisabeth Bermejo-Padilla a. Dr. Jon K. Pittman b, Prof. Constantinos Theodoropoulos a a Department of Chemical Engineering and Analytical Science, Biochemical and Bioprocess Engineering Group b Department of Earth and Environmental Sciences The University of Manchester, Manchester, UK, M13 9PL This publication is part of the deliverables of the Interreg-funded international project EnhanceMicroAlgae. The authors gratefully acknowledge the European Regional Development Fund (ERDF) Interreg Atlantic Area programme which funded the EnhanceMicroAlgae project: EAPA_338/2016, “High added-value industrial opportunities for microalgae in the Atlantic Area”. Microalgae production for high added value compounds is identified as a business sector with high growth potential in the coming decades, especially in the Atlantic Area. Barriers to improve an industrial use are dominated by a lack of technology expertise. The EnhanceMicroAlgae project aims to stimulate research, innovation, industrial development and transnational cooperation within the Atlantic area microalgae sector. The main objective is to contribute to the competitiveness of microalgal-based industries in the Atlantic Area. CONTENTS Introduction ................................................................................................................................ 5 Important notes .......................................................................................................................... 6 1. Anabaena cylindrica .............................................................................................. 7 2. Ankistrodesmus falcatus ..................................................................................... 10 3. Arthrospira platensis ........................................................................................... 12 4. Auxenochlorella protothecoides .......................................................................... 17 5. Botryococcus braunii ........................................................................................... 19 6. Chaetoceros calcitrans ........................................................................................ 22 7. Chlamydomonas reinhardtii ................................................................................ 26 8. Chlorella sorokiniana ........................................................................................... 30 9. Chlorella vulgaris ................................................................................................. 33 10. Chromochloris zofingiensis .................................................................................. 38 11. Crypthecodinium cohnii ....................................................................................... 40 12. Desmodesmus subspicatus .................................................................................. 42 13. Dunaliella salina .................................................................................................. 45 14. Dunaliella tertiolecta ........................................................................................... 50 15. Euglena gracilis ................................................................................................... 53 16. Galdieria sulphuraria ........................................................................................... 55 17. Graesiella sp. ....................................................................................................... 57 18. Haematococcus pluvialis ..................................................................................... 59 19. Isochrysis galbana ............................................................................................... 63 20. Jaagichlorella luteoviridis .................................................................................... 67 21. Lyngbya lutea ...................................................................................................... 69 22. Microchloropsis salina ......................................................................................... 71 23. Microcystis aeruginosa ........................................................................................ 74 24. Nannochloropsis oculata ..................................................................................... 76 25. Nostoc sp. ............................................................................................................ 80 26. Odontella aurita .................................................................................................. 83 27. Parachlorella kessleri ........................................................................................... 86 28. Picochlorum sp. ................................................................................................... 89 29. Porphyridium purpureum .................................................................................... 91 30. Phaeodactylum tricornutum ............................................................................... 95 31. Rhodomonas sp. ................................................................................................ 100 32. Scenedesmus obliquus ....................................................................................... 107 33. Scenedesmus quadricauda ................................................................................ 111 34. Selenastrum capricornutum .............................................................................. 114 35. Tetraselmis subcordiformis ............................................................................... 116 36. Tetraselmis suecica ........................................................................................... 120 37. Tisochrysis lutea ................................................................................................ 124 Appendix 1. Media recipes ..................................................................................................... 127 A.1. Artificial Seawater (ASW) medium ....................................................................... 128 A.2. Blue-Green medium (BG11) .................................................................................. 129 A.3. Bold’s Basal Medium (BBM) and 3N-BBM ............................................................ 130 A.4. Chu 13 medium (Modified) ................................................................................... 132 A.5. Conway medium ................................................................................................... 133 A.6. Detmer medium (DM) modified ........................................................................... 134 A.7. f/2 medium ........................................................................................................... 135 A.8. f/2+Si (Guillard’s medium for diatoms) ................................................................ 136 A.9. Jaworski’s Medium (JM) ....................................................................................... 138 A.10. Kuhl medium ...................................................................................................... 139 A.11. SOT medium ....................................................................................................... 140 A.12. Sueoka medium .................................................................................................. 141 A.13. Walne’s medium ................................................................................................. 142 A.14. Zarrouk medium ................................................................................................. 143 Appendix 2. Culture collections .............................................................................................. 144 Appendix 3. List of images ...................................................................................................... 145 References .............................................................................................................................. 147 Page | 4 Introduction Microalgae are a broad group of diverse microorganisms that are typically single-celled, photosynthetic organisms that derive from marine, brackish, freshwater or terrestrial