US010682400B2 ( 12 ) United States Patent (10 ) Patent No.: US 10,682,400 B2 Ali et al. (45 ) Date of Patent : Jun . 16 , 2020 ( 54 ) COMBINATION VACCINE DEVICES AND (2013.01 ) ; A61K 2039/55561 ( 2013.01) ; COOK METHODS OF KILLING CANCER CELLS 2317/21 (2013.01 ) ; CO7K 2317/24 (2013.01 ) (71 ) Applicants : President and Fellows of Harvard (58 ) Field of Classification Search College , Cambridge , MA (US ) ; CPC A61K 39/39 ; A61K 39/0011 Dana - Farber Cancer Institute , Inc., See application file for complete search history. Boston , MA (US ) (72 ) Inventors : Omar Abdel -Rahman Ali, Cambridge, ( 56 ) References Cited MA (US ) ; David J. Mooney, Sudbury, U.S. PATENT DOCUMENTS MA (US ) ; Glenn Dranoff , Lexington , MA (US ) 3,773,919 A 11/1973 Boswell et al . 4,522,811 A 6/1985 Eppstein et al . 4,946,778 A 8/1990 Ladner et al. ( 73 ) Assignees : President and Fellows of Harvard 5,073,627 A 12/1991 Curtis et al. College, Cambridge , MA (US ) ; 5,091,513 A 2/1992 Huston et al . 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PCT Pub . Date : Nov. 5 , 2015 ( Continued ) (65 ) Prior Publication Data FOREIGN PATENT DOCUMENTS US 2017/0042995 A1 Feb. 16 , 2017 AU 2018201930 Al 4/2018 CN 1757662 A 4/2006 Related U.S. Application Data (Continued ) (60 ) Provisional application No.61 / 986,600 , filed on Apr. OTHER PUBLICATIONS 30 , 2014 . Hodi et al. ( N England J Med 2010 ; 363 :711-723 ) ( Year: 2010 ). * ( 51 ) Int. Cl. Quintana and Cohen ( J. Autoimmunity 2001 17 : 191-197 ) ( Year : A61K 39/00 ( 2006.01 ) 2001 ) . * COZK 16/28 (2006.01 ) Duraiswanny et al. ( Cancer Research Jun . 2013 73 ( 12 ) : 3591-3603 ) COOK 16:30 ( 2006.01 ) ( Year: 2013 ) . * A61K 39/12 ( 2006.01 ) (Continued ) A61K 39/39 ( 2006.01 ) A61K 39/395 ( 2006.01) A61K 47/34 ( 2017.01 ) Primary Examiner - Peter J Reddig A61K 4736 ( 2006.01 ) (74 ) Attorney , Agent, or Firm — McCarter & English , A61K 39/02 ( 2006.01) LLP ; Maria Laccotripe Zacharakis ; Maneesh Gulati C12N 7700 ( 2006.01) (52 ) U.S. CI. CPC A61K 39/0011 ( 2013.01 ) ; A61K 39/02 ( 57 ) ABSTRACT ( 2013.01 ); A61K 39/12 ( 2013.01 ) ; A61K 39/39 (2013.01 ) ; A61K 39/3955 ( 2013.01 ) ; A61K The present invention comprises compositions, methods, 47/34 ( 2013.01 ) ; A61K 47/36 (2013.01 ) ; CO7K and devices for enhancing an endogenous immune response 16/2803 ( 2013.01 ) ; CO7K 16/2818 ( 2013.01) ; against a cancer. Devices and methods provide therapeutic CO7K 16/30 (2013.01 ) ; CO7K 16/3053 immunity to subjects against cancer. ( 2013.01 ) ; C12N 7700 (2013.01 ) ; ACIK 2039/507 ( 2013.01 ) ; A61K 2039/5152 ( 2013.01) ; A61K 2039/54 (2013.01 ) ; A61K 26 Claims, 13 Drawing Sheets 2039/55516 (2013.01 ) ; A61K 2039/55522 Specification includes a Sequence Listing . US 10,682,400 B2 Page 2 ( 56 ) References Cited 2005/0154376 A1 7/2005 Riviere et al . 2005/0177249 A1 8/2005 Kladakis et al . 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