2019 - 2020 Catalog Microbiomics About the Cover Who We Are by Christopher E. Mason, Ph.D. In the past two years, extraordinary new research in the microbiome and metagenome fields have shown a wide range of functions, plasticity, and novel applications. From sequencing DNA on the International Space Station in the Biomolecular Sequencer (BSeq) Mission1, to sequencing thousands of subway stations in the Metagenomics of Subways and Urban Biomes (MetaSUB) project2, to discovering completely novel genera that drive the risks of diabetes in The Environmental Determinants of Diabetes in the Young (TEDDY) project3, many new breakthroughs are constantly being made. These breakthroughs include new metrics and risk factors for diabetes from early childhood onward, new risk Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing stratification criteria for adult diabetes patients, and new deployment of NGS innovative, reliable, and high quality research tools at affordable prices. Our vision “The Beauty of Science methods in microbiome research for clinicians. is to Make Things Simple” is now truer than ever. Whether it’s epigenetics, DNA, RNA, E. coli, or yeast based research, our philosophy remains the same: To provide the highest quality products in the industry Notably, the ZymoBIOMICS® microbial reference materials (see pages 150-153) while ensuring they are both simple to use and reliable in their performance. have been used for more and more studies as critical positive controls. Several groups have now established both the genetic and epigenetic landscapes of these Zymo Research stands on three pillars which form the foundation of our company: Innovation, Quality, and microbial standards. These landscapes reflect work from the Loman and Mason Customer Service. These pillars are fundamental to our culture and ensure our products meet your needs. Labs, which are freely and publicly available for use by other groups. Based on Oxford Nanopore Technologies, PacBio, and Illumina sequencing, these genome Innovation assembly metrics and specific sites of base modifications can ensure robust data interpretation for limits of detection. In addition, titrated abundance can be Zymo Research is historically recognized for its innovation of high quality nucleic acid purification estimated from the release of the new, log-titrated ZymoBIOMICS® Standards. technologies. Under the branding DNA Purification Made Simple® and RNA Purification Made Simple®, These data can help in many areas, including rapid iteration for technology development in Next-Gen Sequencing for genomics and our technologies are pushing the limits of what is possible with nucleic acid isolation. As The Epigenetics epigenomics, process controls for large-scale data projects, and variant-calling and assembly algorithm development. Company™, Zymo Research has also received much attention for its rapidly expanding portfolio of epigenetics products and services. It is our objective to develop and provide the most comprehensive set Notably, some genomes in the ZymoBIOMICS® standards have scant or undetectable levels of modifications like methyl-6-adenine of tools for DNA, RNA, and epigenetic research and analysis available today. Thousands of peer-reviewed (m6A), whereas others have high or wide-ranging levels of m6A and 5-methylcytosine (5mC). Since new strains can show distinct scientific publications from researchers around the world feature our technologies. Through innovation, and different levels of these epigenetic marks, the ZymoBIOMICS® standards are all the more important as reference materials. our scientists have made streamlined DNA methylation detection possible, pioneered the micro-elution This work is similar to the “meter stick of the genome” efforts of the Genome in a Bottle Consortium4 and the Global Alliance for column for DNA and RNA purification, developed the simplest and the most sophisticated methods for Genomics and Health (GA4GH)5, who are helping to adjudicate the metrics and parameters needed for accurate genetic variant calls high-quality plasmid DNA purification, and patented the first RNA purification directly from Trizol® without in human genome sequencing. Going forward, these “metagenomes in a bottle” represent well-curated and validated metagenome phase separation among many other leading technologies in the industry. standards that set the stage for in-depth and accurate studies of the microbiome, help improve genome assembly tools, and ensure greater reproducibility and interpretability for scientists and clinicians alike. Quality We are committed to quality and guarantee that all of our products and service will meet and exceed your expectations. Our products are constantly evaluated by scientists like you to help ensure their reliability and the highest standard of quality. About Christopher E. Mason, Ph.D. Dr. Christopher Mason is currently an Associate Professor at Weill Cornell Customer Service Medicine, with appointments at the Tri-Institutional Program in Computational Biology and Medicine between Cornell, Memorial Sloan-Kettering Cancer Center and Rockefeller University, the Sandra and Edward Meyer Cancer We strive for excellence in how we support your scientific endeavors. We pledge to be honest and Center, and the Feil Family Brain and Mind Research Institute. The Mason responsible for everything we do with you. We will treat you as we would like to be treated. Together, we laboratory is working on a ten-phase, 500-year plan for the survival of the will build a brighter future. human species on Earth, in space, and on other planets. Excitingly, all the details, methods, and data are available for immediate use: • https://www.biorxiv.org/content/early/2017/04/13/127100 • https://www.biorxiv.org/content/early/2018/12/10/487033 • https://www.ncbi.nlm.nih.gov/biosample?Db=biosample&DbFrom=bioproject&Cmd=Link&LinkName=bioproject_ biosample&LinkReadableName=BioSample&ordinalpos=1&IdsFromResult=477598 • https://www.fda.gov/medicaldevices/scienceandresearch/databaseforreferencegrademicrobialsequences/default.htm References: 1. Castro-Wallace, S.L., et al. “Nanopore DNA Sequencing and Genome Assembly on the International Space Station.” Scientific Data. 2017 Dec 21;7(1):18022. 2. The MetaSUB International Consortium. The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium inaugural meeting report. Microbiome. 2016 Jun 3;4(1):24. 3. Vatanen, T., et al. The human gut microbiome in early-onset type 1 diabetes from the TEDDY study. Nature. 2018 Oct;562(7728):589-594. 4. Genome in a Bottle Consortium: http://jimb.stanford.edu/giab/ 5. Global Alliance for Genomics and Health (GA4GH): https://www.ga4gh.org Artistic Rendition by Jay Chen and Casey Cruz Ordering Information Contents Address Returns Zymo Research Corp. Goods may not be returned for credit except with Seller’s (i.e., Zymo Research Epigenetics 4 E. coli 164 17062 Murphy Ave Corporation) permission, and then only in strict compliance with Seller’s return DNA/RNA Methylation Irvine, CA 92614, USA shipment instructions. Returned goods (other than defective products) must be DNA Hydroxymethylation Chemically Competent returned freight prepaid by the customer and will not be accepted without prior Chromatin Analysis Transformation Reagents Payment Method authorization of Zymo Research. The goods must be returned in their original Purchase order or Visa/Mastercard/AMEX only. packaging and in resalable condition. Certain items may not be returned for credit. 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