Nitrogen Fixation for Agriculture and Environment Proceedings of the 20th International Congress on Nitrogen Fixation Granada, Spain, 3-7 September 2017 Congress organized by: Estación Experimental del Zaidín, Universidad de Granada (UGR) Consejo Superior de Investigaciones Científicas (EEZ-CSIC) Congress Sponsored by: Collaborators: Local organizing committee Chair: María J. Delgado. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Granada, Spain Co-chairs: Eulogio J. Bedmar Gómez. EEZ, CSIC, Spain Socorro Mesa Banqueri . EEZ, CSIC, Spain Juan Sanjuán Pinilla. EEZ, CSIC, Spain José I. Jiménez-Zurdo. EEZ, CSIC, Spain José A. Herrera-Cervera. Plant Physiology Department, University of Granada (UGR), Spain Carmen Lluch Plá. Plant Physiology Department, UGR, Spain Jesús González-López. Microbiology Department, UGR, Spain Belén Rodelas González. Microbiology Department, UGR, Spain International steering committee Barbara Reinhold-Hurek. University of Bremen, Germany Esperanza Martínez-Romero. Centro de Ciencias Genómicas, México Felix Dakora. Tshwane University of Technology, Republic of South Africa Graham O'Hara. Murdoch University, Australia John Peters. Montana State University, USA Igor Tikhonovich. All-Russia Research Institute for Agriculture and Microbiology, Russia Kiwamu Minamisawa. Tohoku University, Japan Ray Dixon. John Innes Research Centre, United Kingdom Sharon R. Long. Stanford University, USA William Newton. Virginia Tech, USA Yi-Ping Wang. Peking University, China Luis M. Rubio. Universidad Politécnica de Madrid/CSIC, Spain Program advisory committee Anke Becker. University of Marburg, Germany Ann M. Hirsch. UCLA, USA Catherine Masson-Boivin. INRA, France César Arrese-Igor. Universidad Pública de Navarra, Spain Chris Voigt. MIT, USA Dulce N. Rodríguez. IFAPA, Spain Eustoquio Martínez Molina. Universidad de Salamanca, Spain Hans-Martin Fischer. ETH-Zürich, Switzerland J. Peter W. Young. University of York, United Kingdom James N. Galloway. University of Virginia, USA Jean-Jacques Drevon. INRA, France Jens Stougaard. Aarhus University, Denmark José M. Vinardell. Universidad de Sevilla, Spain Juan Imperial. Universidad Politécnica de Madrid/CSIC, Spain Lourdes Girard. Centro de Ciencias Genómicas, México Luis M. Rubio. Universidad Politécnica de Madrid/CSIC, Spain Manuel Becana. Estación Experimental Aula Dei, CSIC, Spain Mariangela Hungría. EMBRAPA, Brazil Michael Udvardi. The Samuel Roberts Noble Foundation, USA Stephen P. Cramer. University of California, USA David J. Richardson. University of East Anglia, United Kingdom Technical secretary Rafaela García (Director) Arancha Campos (Congress Organizer) Beatriz Moreno (Congress Organizer) Congresos Gestac S.L. C/ San Antón, 72 1º Dcha. 18005 GRANADA – SPAIN –+34 958 132 949 [email protected] Congress website http://20icnf.congresosgestac.com/es/ Index Scientific program 7 Keynote speakers Opening lecture 20 Closing lecture 21 Plenary Sessions Plenary Session 1: Nitrogen, Environment and Global Change 22 PS1-01 to 03 Plenary Session 2: Evolution, Diversity and Ecology of 26 Diazotrophs PS2-01 to 03 Plenary Session 3: Chemistry, Biochemistry and Biotechnology of 30 Nitrogenase PS3-01 to 03 Plenary Session 4: Regulation in Nitrogen Fixing Organisms 34 PS4-01 to 03 Plenary Session 5: Physiology of Nitrogen Fixing Associations 38 PS5-01 to 03 Plenary Session 6: Plant-Microbe Signaling 42 PS6-01 to 03 Plenary Session 7: Plant Physiology and Crop Productivity 46 PS7-01 to 03 Plenary Session 8: Inoculant Production and Applications. 50 Novel Uses and Engineering of Nitrogen-Fixing Organisms PS8-01 to 03 Parallel Sessions Parallel Session 1: Nitrogen Cycle and Global Change Oral presentations PAS1-O01 to O05 54 Poster presentations PAS1-P01 to P07 60 Parallel Session 2: Evolution, Diversity and Ecology of Nitrogen- Fixing Organisms Oral presentations PAS1-O01 to O05 68 Poster presentations PAS1-P01 to P25 74 Index 5 Parallel Session 3: Chemistry, Biochemistry and Biotechnology of Nitrogenase Oral presentations PAS3-O01 to O05 100 Poster presentations PAS3-P01 to P04 106 Parallel Session 4: Regulation in Nitrogen Fixation Systems Oral presentations PAS4-O01 to O07 111 Poster presentations PAS4-P01 to P23 119 Parallel Session 5: Functioning of Legume-Rhizobium Symbiosis Oral presentations PAS5-O01 to O08 143 Poster presentations PAS5-P01 to P22 152 Parallel Session 6: Non-legume Nitrogen-Fixing Associations Oral presentations PAS6-O01 to O05 175 Poster presentations PAS6-P01 to P16 181 Parallel Session 7: Plant-Microbe Signaling Oral presentations PAS7-O01 to O11 198 Poster presentations PAS7-P01 to P53 210 Parallel Session 8: Environmental Constraints to Nitrogen Fixation and Crops Productivity Oral presentations PAS8-O01 to O05 264 Poster presentations PAS8-P01 to P22 270 Parallel Session 9: Inoculant Production and Applications Oral presentations PAS9-O01 to O05 293 Poster presentations PAS9-P01 to P17 299 Parallel Session 10: Novel Uses and Engineering of Nitrogen- Fixation Systems Oral presentations PAS10-O01 to O05 317 Poster presentations PAS10-P01 to P07 323 List of Congress participants 331 Index 6 Scientific program 3rd September (Sunday) 12:00-18:00 Registration OPENING CEREMONY (Aula Magna, Faculty of Sciences) 18:00-18:30 Welcome address 18:30-19:30 Opening lecture William Newton (Virginia Tech, Blacksburg, Virginia, USA) “The international congresses and nitrogen-fixation research: Retrospect and prospects” 20:00-22:00 Welcome reception 4th September (Monday) PLENARY SESSION 1: Nitrogen, environment and global change (Aula Magna, Faculty of Sciences) Chairs: Mª Dolores Roldán and Jesús González-López 08:30-09:00 David J. Richardson (University of East Anglia, Norwich, UK) “Producing and destroying a potent greenhouse gas” 09:00-09:30 Jan W. Erisman (Louis Bolk Instituut, Driebergen, The Netherlands) “Nitrogen: too much of a vital resource” 09:30-10:00 Maren Voss (Leibniz Institute of Baltic Sea Research, Rostock, Germany) “Anthropogenic impact on the ocean” 10:00-10:30 Coffee break Scientific Program 7 PLENARY SESSION 2: Evolution, diversity and ecology of diazotrophs (Aula Magna, Faculty of Sciences) Chairs: Álvaro Peix and Eulogio J. Bedmar 10:30-11:00 Catherine Masson-Boivin (Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France) “Experimental evolution of mutualism in the legume-rhizobium symbiosis” 11:00-11:30 Wiebke Mohr (Max Planck Institute for Marine Microbiology, Bremen, Germany) “N2-fixing symbioses in the marine environment: On key players and how to find them” 11:30-12:00 Esperanza Martínez-Romero (CCG-UNAM, Cuernavaca, México) “Nitrogen fixation in insects” 12:00-14:30 Lunch break PLENARY SESSION 3: Chemistry, biochemistry and biotechnology of nitrogenase (Aula Magna, Faculty of Sciences) Chairs: John Peters and Ray Dixon 14:30-15:00 Luis M. Rubio (Universidad Politécnica de Madrid, Madrid, Spain) “Advances in engineering eukaryotic nitrogenase” 15:00-15:30 Lance C. Seefeldt (Utah State University, Logan, USA) “Insights into the nitrogenase mechanism” 15:30-16:00 SanFeng Chen (China Agricultural University, Beijing, China) “Nitrogen fixation in Paenibacillus polymyxa WLY78” 16:00-16:30 Coffee break PARALLEL SESSION 1: Nitrogen cycle and global change (Lecture Room, School of Engineering, ETSI) Chair: David Richardson 16:30-17:00 Sara Hallin (Swedish University of Agricultural Sciences, Uppsala, Sweden) “Ecology of N2O reducing bacteria and archaea – the main N2O sink in the biosphere” Scientific Program 8 17:00-17:15 Bryan Vincent (IRD, New Caledonia & CIRAD, Montpellier, France) “Naturally occurring Acacia spirorbis in New Caledonia: a top- notch nitrogen-fixing species through extremely contrasted soils” 17:15-17:30 Alba Hidalgo-García (EEZ-CSIC, Granada, Spain) “The assimilatory nitrate reductase is required for denitrification in the Rhizobium etli – common bean symbiosis” 17:30-17:45 María J. Torres (EEZ-CSIC, Granada, Spain) “FixK2 is the transcriptional activator of Bradyrhizobium diazoefficiens nosRZDYFLX genes in response to low oxygen” 17:45-18:00 Cristina Sánchez (Graduate School of Life Sciences, Tohoku University, Japan) “Redundant roles of Bradyrhizobium oligotrophicum Cu-type and cd1-type nitrite reductase genes under denitrifying conditions” PARALLEL SESSION 2: Evolution, diversity and ecology of nitrogen-fixing organisms (Aula Magna, Faculty of Sciences) Chair: Maren Friesen 16:30-17:00 Euan K. James (The James Hutton Institute, Dundee, UK) “Biogeography of legume nodulation by Beta-rhizobia” 17:00-17:15 Vincent R. G. Greffe (Katholieke Universiteit Leuven, Leuven, Belgium) “Characterization of phenotypic and genotypic diversity of Sinorhizobium meliloti strains” 17:15-17:30 Anastasiia K. Kimeklis (All-Russia Research Institute for Agricultural Microbiology, St. Petersburg, Russia) “Divergence of nucleotide sequences of symbiotic genes indicates the early stages of speciation in Rhizobium leguminosarum bv. viciae isolated form relict legume Vavilovia formosa” 17:30-17:45 Pilar Martínez-Hidalgo (Universidad de Salamanca, Salamanca, Spain) “The diversity in Medicago truncatula root nodules phytomicrobiome” 17:45-18:00 J. Peter W. Young (University of York, York, UK) “Population genomics of Rhizobium leguminosarum” 18:00-20:00 POSTER SESSION 1 (odd numbers) 20:00-23:30 “Granada tapas tour” (optional activity) Scientific Program 9 5th September (Tuesday) PLENARY SESSION 4: Regulation in nitrogen-fixing organisms (Aula Magna,