Key to respiratory pathology Aperio histology cases - Diagnoses Title Species Diagnosis Case number Case details 03-33330A cytokeratin Bovine Cytokeratin-normal lung 03-33330B cytokeratin Bovine Cytokeratin-normal lung 07-107482-A1 Porcine Swine influenza Swine influenza--with nice IHC. 38514-98 Wild boar Metastrongylus 98-38514-5 Feedlot steer, bronchiolitis obliterans, as a sequel to viral bronchiolar necrosis, with arteriolar hypertrophy and 97-3591 Bovine Bronchiolitis fibrosa obliterans heart failure 98-887-20A 20hrs pi 20 hours after Mannheimia challenge 98-887-20B 20hrs pi 20 hours after Mannheimia challenge 98-887-3A 3hrs pi 3 hours after Mannheimia challenge 98-887 Lambs with low antibody titres, challenged by aerosol with PI3V then 6 x 10^8 cfu of M. haemolytica. 98-887-3B 3hrs pi 3 hours after Mannheimia challenge 98-887-8A 8hrs pi 8 hours after Mannheimia challenge 98-887-8B 8hrs pi 8 hours after Mannheimia challenge ACVP-81 Equine Silicosis Slide from an old ACVP meeting. Gross postmortem examination reveals multifocal, well-demarcated, pale, bulging, solid regions within all lung lobes (from <1 to 4cm dia). Contained airways exude frothy fluid although only small amounts of such fluid is noted in the mainstem bronchi or trachea. Histopathological examination of affected lung reveals multifocal, unencapsulated regions where the alveolar architecture is obscured by complex acinar & papillary arrangements of well regimented columnar cells on thin fibrovascular septi. These cells are densely packed, have pale eosinophilic to clear cytoplasm & basally orientated vesicular nuclei. Mitotic figures are inconspicuous. Infiltrates of neutrophils & macrophages are noted within contained & adjacent airspaces. No significant D08-26 Ovine Jaagsiekte N76/08 abnormalities noted within mediastinal lymph node sample. Incidental finding in the lung of an adult horse. The lung lesion is well-demarcated and delineated by a fibrous capsule, with an area of mature fibrosis in the adjacent lung tissue and pleura. The wall of the cyst is formed by a laminated layer of basophilic mucinous material, and an inner layer of cuboidal cells. The innermost aspect contains innumerable multifocal 100-um aggregates of mineralized debris, and rare similarly sized structures N124108A Equine Echinococcus N124108A with viable cells and several refractile hooks (indicating that they are protoscolices of Echinococcus granulosus). Dog was attacked and shaken by another dog. The lung is markedly congested and edematous, and close inspection reveals many thin hyaline membranes lining the surface of the alveoli. Alveolar hemorrhage is present but mild. This is an example of acute respiratory distress syndrome, probably as a result of trauma, PB-Ca8 Canine Diffuse alveolar damage (ARDS), 2ry to trauma 04-60164-A asphyxiation, or both. PB-Ca9 Bovine Endotoxemic lung injury 00-16766-2 Endotoxemic lung injury. Cow, acute mastitis, dyspnea 12 hours after calving. Cow. Massive pulmonary embolism from a thrombus in the uterine vein. Cow at Elora was examined and treated by 4th year vet students, who then went elsewhere in the barn to treat another cow. When they returned, the cow was dead, and necropsy revealed metritis with a thrombus in the uterine vein, and massive PB-Cb4 Bovine Pulmonary embolus 67805-2 pulmonary embolism. Dog. Interstitial fibrosis secondary to chronic heart failure. 10 year old Calvalier King Charles Spaniel, with a chronic history of mitral valve disease. Gross: left AV valvular endocardiosis, left atrial dilatation, left PB-Cb5 Dog Interstitial fibrosis 2ry to chronic heart failure. 96-514-5 ventricular eccentric hypertrophy, one ruptured chorda tendinae, and pulmonary edema and congestion. PB-Cc100 Canine Blastomycosis Experimental infection with Nipah virus. Attenuation of tracheal surface epithelium, with lymphocytic tracheitis and moderate numbers of eosinophils, lymphocytes cluster within tracheal epithelium. Patchy suppurative exudate within the lumen. One area has single cell necrosis of epithelial cells, and possible PB-Cc103 Pig Nipah virus: tracheal epithelial necrosis 04-303-40B inclusions (or uptake of apoptotic cells?). PB-Cc104 Calf Embolic pneumonia 2ry to endocarditis 65151-2 Embolic pneumonia 2ry to endocarditis PB-Cc105 Dog Cryptococcosis 02-32154-13 PB-Cc94a PB-Cc94b PB-Cc94c PB-Cc96 Cat? Idiopathic pulmonary fibrosis? Page 1 of 13 Key to respiratory pathology Aperio histology cases - Diagnoses Title Species Diagnosis Case number Case details PB-Cc98 Horse? Heaves? PB-Cd11a Feline Pulmonary adenocarcinoma nasal adenocarcinoma; has had unilateral nasal disease for >1 year and a diagnosis of lymphoplasmacytic rhinitis PB-Cd12 Canine Nasal adenocarcinoma YB133947 last year PB-Cf13 Feline Idiopathic pulmonary fibrosis 05-15413 chronic interstitial pneumonia / idiopathic pulmonary fibrosis with pulmonary carcinoma Chronic proliferative interstitial pneumonia/idiopathic pulmonary fibrosis. 8 year old cat. Born in South Africa. Hiding, stopped eating. Vague clinical signs. Also has severe hepatic lipidosis. Histo: Irregular distribution of chronic interstitial lung disease with type II pneumocyte proliferation, remarkable hyperplasia/metaplasia of smooth muscle, and mild fibrosis. Fragments of blue-gray material are present in PB-Cf14 Feline Idiopathic pulmonary fibrosis, smooth muscle hyperplasia D05-06 alveoli (rule out inhalation of bentonite-cat litter dusts as pneumoconiosis?). YB 132809. PB-Cf15 Bovine Normal bovine lung 97-2054 PB-Cf16 Canine Normal canine lung D09-04 The lung is immature with an excess of connective tissue in the alveolar septa. Many of the alveoli are lined by hyaline membranes, and cuboidal epithelium lining the hyaline membranes is occasionally still present (probably has not yet differentiated to type 1 pneumocytes, rather than a reversion). Keratin squames are present in the PB-RESP-1 Puppy, 1 d old Puppy. Neonatal ARDS / hyaline membrane disease 02-D14 alveoli. The the fine black pigment within some cells is acid hematin (artifactual). Renal disease last year, renal transplant, great postop. One month hx lethargy, 2 wk hx anorexia/inappetance. Developed dyspnea 5d ago, Chest rads - patchy diffuse infiltrates. Cyclosporin level very elevated (1500!). PM: lung is diffusely firm and has multifocal coalescing white consolidated depressed areas (0.5 - 3 cm), mostly present in the cranioventral lobes and rarely in the caudodorsal parts. On cut section, lung has a dry congested surface that alternates with the white consolidated areas. Also multifocal hepatic necrosis. Histo: Multifocal random lesions of inflammation and necrosis, in which alveoli are lined by cuboidal type 2 pneumocytes and filled with necrotic cellular debris, basophilic chromatin debris, neutrophils, macrophages. The epithelial cells contain large numbers of protozoal cysts, which are up to 25 microns diameter and contain numerous zoites. PB-RESP-2 Cat Cat. Pulmonary toxoplasmosis 02-31610-2 Multifocal necrotizing and proliferative interstitial pneumonia with protozoal cysts. 13-yr-old dog, incidental finding.The pulmonary tumor is poorly demarcated, non-encapsulated, and occupies most of the section in those affected. In many areas, the arrangement of neoplastic cells mimics that of normal alveoli -- the tumor forms multiple epithelial-lined air spaces separated by fibrous trabeculae. The neoplastic cells are cuboidal, with indistinct borders, scant eosinophilic cytoplasm, round nuclei with finely clumped chromatin, 2-fold anisokaryosis, and rare mitotic figures. A prominent feature of the tumor is the presence of 5- 100 micron diameter concretions of brightly eosinophilic material (surfactant-like proteins?) within the air spaces. Occasionally, neoplastic epithelial cells tightly encircle this material, suggesting that it is being secreted Dog. Pulmonary adenocarcinoma; some features of by the tumor cells. In addition, there are numerous macrophages, occasional neutrophils, and cellular debris in PB-RESP-3 Dog bronchioloalveolar carcinoma D03-92-1 the air spaces. The areas in which alveoli are Adult dog, unilateral bloody nasal discharge. The tissue consists entirely of plaques of fungal hyphae which PB-RESP-5 Dog Dog. Nasal aspergillosis 93-2024 show features characteristic of Aspergillus: parallel walls, 4-micron-diameter, dichotomous branching, Bronchioles are markedly dilated and there is corresponding atelectasis of the alveoli. The bronchioles often contain neutrophils, with occasional erosion of the epithelium. In some bronchioles, the brush border is unexpectedly dense and slightly basophilic. A Warthin Starry silver stain reveals that this is the result of many PB-RESP-6 Rat CAR bacillus with bronchiectasis. D05-109. YB146764 bacilli adherent to the cilia. Mycoplasma pneumoniae is a differential diagnosis for bronchiectasis in rats. Bronchioles are markedly dilated and there is corresponding atelectasis of the alveoli. The bronchioles often contain neutrophils, with occasional erosion of the epithelium. In some bronchioles, the brush border is unexpectedly dense and slightly basophilic. A Warthin Starry silver stain reveals that this is the result of many PB-RESP-7 Rat CAR bacillus --Warthin Starry stain of above. D05-109. YB146764 bacilli adherent to the cilia. Mycoplasma pneumoniae is a differential diagnosis for bronchiectasis in rats. Several of the airways are tremendously dilated and occupy the majority of the section. These are filled with an exudate of neutrophils, the epithelial lining has undergone squamous