Michael F. Romero, PhD CURRICULUM VITAE September 2, 2011 Personal Data Hispanic Born, Atlanta, Georgia, USA Dept. Physiology & Bio medical Engineering Mayo Clinic College of Medicine 200 First St SW, Rochester, MN 55905 Email [email protected] Tel. 507-284-8127 url: http://mayoresearch.mayo.edu/mayo/research/romero_lab/ http://discoverysedge.mayo.edu/de09-1-kid-romero-hco3transporters/ Education Undergraduate: Thomas More College, Crestview Hills, KY. 1984 BA: Biology Associate Degree: Chemistry and Mathematics Graduate: Case Western Reserve University, Cleveland, Ohio 1984-88 Dept. Developmental Genetics & Anatomy 1988-91 Dept. Genetics (official) 1986-91 Dept. Physiology & Biophysics (lab relocation) 1/1992 PhD October 4, 1991 (CWRU granted PhD Jan. 1992) Research advisor: Ulrich Hopfer, MD, PhD Thesis Title: Angiotensin II regulation of ion transport in the rabbit proximal tubule Professional Experience 11/91-2/92 Post Doctoral Fellow Dept. Physiology & Biophysics, Case Western Reserve Univ, Cleveland, OH 2/92-7/95 Post Doctoral Fellow Dept. Cellular & Molecular Physiology, Yale Univ., New Haven CT 7/95-9/97 Associate Research Scientist1 Dept. Cellular & Molecular Physiology, Yale Univ., New Haven CT 9/97- 6/03 Assistant Professor Dept. Physiology & Biophysics, Case Western Reserve Univ, Cleveland, OH 10/98-6/03 Assistant Professor Dept. Pharmacology, CWRU 7/03 – 8/31/06 Associate Professor Dept. Physiology & Biophysics, CWRU 7/03 – 8/31/06 Associate Professor Dept. Pharmacology, CWRU 7/1/06 Award of Tenure Case Western Reserve Univ, Cleveland, OH 9/1/06 – present Associate Professor / Dept. Physiology & Biomedical Engineering Sr. Associate Consultant Mayo Clinic College of Medicine 4/20/07-present Associate Professor / Div. Nephrology & Hypertension Sr. Associate Consultant Mayo Clinic College of Medicine 1 Associate Research Scientist is a non-tenure track faculty appointment. MF Romero Curriculum vitae - September 2, 2011 2 Personal Statement My background is renal physiology, electrophysiology and ion / solute transporter biophysics. As a postdoctoral fellow with Walter Boron, I used an expression cloning technique with Xenopus oocytes to clone the first cDNA for a + - Na /HCO3 cotransporter (NBCe1, Slc4a4). We have collaborated with Matthias Hediger and several other investigators - + to clone and characterize the transport activities of the SLC solute carriers, emphasizing HCO3 , anion and Na transport. - - Several years ago with Dr. David Mount (Brigham), we cloned and characterized novel electrogenic Cl /HCO3 exchangers of the Slc26 family. Since then, we have used human mutations (or cSNPs) to find how function is altered. We have taken an evolutionary approach to follow our Slc4 and Slc26 genes through evolutionary time, by cloning and expressing ortholog clones particularly from organisms which adapt to unique environments (e.g., euryhaline teleosts, and Diptera insects). Before leaving Yale, I identified and initially characterized a C. elegans Slc4 cDNA + - (ceNBC, Genbank#AF004926) as an electroneutral Na /HCO3 cotransporter (Romero MF, Boron WF. J Am Soc + - - Nephrol 9: 11A, 1998). In 1998 my lab cloned the first Na driven Cl /HCO3 exchanger (NDAE1) from Drosophila. We spent several years using this insect clone to understand mammalian transport. In 2000 I help Fei & Leibach characterize currents and H+ transport by 3 C. elegans H+/oligopeptide transporter (J Biol Chem 275: 9563, 2000). We used NDAE1 overexpression in Sf9 cells for membrane protein purification. Unfortunately, this did not work, but we have maintained an interest in structural biology, through modeling (e.g., N-terminus of NBCe1 – JBC 2007) and protein-protein interactions (CaR/Kir4.1/MuppI – AJP 2007, 2009; Slc26a9 with R-region of CFTR – JBC 2009). Recently we have studied transport function of Slc26a6, Slc26a9 and non-mammalian Slc26a5/prestin. Non-mammalian prestins function - - - 2-- - 2- as Cl /nHCO3 exchangers, Cl /SO4 exchangers and Cl /oxalate exchangers, allowing models of kidney stones 2- (oxalate transport) and malaria (Anopheles SO4 transport) to be developed. We routinely collaborate with laboratories to physiologically characterize ion/solute transporters in Xenopus oocytes (H+ coupled peptide transport, H+ coupled divalent metal transport, H+ coupled folate transport, etc.). Since we normally use glass microelectrodes to determine function of SLC transporters, we are collaborating with Dr. Tian Cui (Mech. Eng., U MN) to biologically verify fabricated, nanosensors to measure ion and solute concentrations. We have - collaborated to physiologically characterize HCO3 transporters (DB Mount, S Hirose, A Kato, P Linser, JA Dow, M Koelle) and H+ transporters (MA Hediger, F Leibach, B Mackenzie, ID Goldman) as well as other transporters and channels (ME Maguire, RT Miller, K Choe, SJ Russell, J Lieske, VA Lennon, MP Fautsch). These combined projects and approaches allow us to continually hone our transporter expertise. Our current projects use our electrophysiology expertise to physiologically characterize transporters and channel proteins in the kidney, gut and eye. These approaches and tissues are being used to understand the transport and environment of acidosis, kidney stones, glaucoma, GABA- ergic signaling and cystic fibrosis. Teaching Experience 1981-84 Speech and Debate, Covington Latin High School, Covington, KY 1985-88 Gross Anatomy teaching, CWRU Medical School, Cleveland, OH Summer ‘93 high school student summer project: electrophysiology Summer ‘95 supervision of high school biology teacher (APS teacher fellowship): molecular biology, Xenopus oocyte expression, high school lab development Summer ‘95 medical student summer project: molecular biology, Xenopus oocyte expression Summer ‘96 2 Dutch medical students summer project: molecular biology, Xenopus oocyte expression, electrophysiology Summer ‘96 2 undergraduates students summer projects (Minority Opportunity Program): molecular biology (subcloning, PCR), Xenopus oocyte expression, electrophysiology Summer ’98 SURP-student, Darin Henry (Edinboro U, PA),: Xenopus oocyte expression, electrophysiology Summer 2001 SURP-student, Brenda Smith (U Akron, OH): Xenopus oocyte expression, electrophysiology Fall 2001 Undergraduate intern, Kristin Bartholomew (Edinboro U, PA); molecular biology, bacterial protein expression Summer 02-04 SURP-student, Jen DiPiero (Duke): molecular biology, Xenopus oocyte electrophysiology Summer 03, 05 2003, high school student, Naina Gupta (Schawnee): Western blotting 2005, undergraduate (U Pennsylvania): molecular biology, Xenopus oocyte electrophysiology Summer 2007 SURF-student, David Ying (MIT): molecular biology, Xenopus oocyte electrophysiology Summer 2008 SURF-student, Alyona Haritonova (St. Katherine’s): Xenopus oocyte electrophysiology Summer 2009 SURF-student, Lauren Brin (St. Katherine’s): Summer 2010 SURF-student, Anna Czapar (Univ Illonois, BME): Summer 2010 SURF-student, Shannon Newman (Laurence College, Biology): Summer 2011 SURF-student, Daniel Bondeson (Univ Wisc., Chemistry) MF Romero Curriculum vitae - September 2, 2011 3 Graduate School Teaching- CWRU PHOL-532 Molecular Organization of Cells. 10/98 “How to study cells.” 2 hr lecture, 12 graduate and 1 undergraduate students 10/99 “How to study cells.” 2 hr lecture, 14 graduate students PHOL-460 (Introduction to Molecular Biology. 11/98 “Xenopus oocyte expression,” 1.5 hr lecture, graduate students. 11/99 “Expression Cloning,” 1.5 hr lecture, graduate students. 11/2/00 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/6/01 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/7/02 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. students. 11/6/03 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. Students (18). (1.5 hr total) 11/04 "Yeast, baculovirus, and oocyte expression; Expression cloning" 1.5 hr, grad. Students (10). (1.5 hr total) 11/05 "Yeast, baculovirus, and oocyte expression; Expression cloning" (1.5 hr total) PHRM 433 (or Physiology 433) Membrane Transport 1999: 5 graduate students 3/24/99 1 hr lecture + 4/7/99 2 hr discussion (3 hr total) 2000: 4 graduate students 11/14/00 1.5 hr lecture 11/16/00 2 hr discussion (3.5 hr total) PHRM 435 (Integrative Systems Physiology and Therapeutics, ISPT) Renal Physiology 2001: 15 graduate students 1/17/01 2 hr lecture 1/22/01 2 hr lecture (4 hr total) 2002: 15 graduate students 2/11/02 2 hr lecture 2/23/02 2 hr lecture (4 hr total) 2003: 2 x 2 hr lectures/discussion as previously (4 hr total) 2004: 2 x 2 hr lectures/discussion as previously (4 hr total) 2005: 2 x 2 hr lectures/discussion as previously 1 x 2 hr original paper discussion (6 hr total) PHOL 398 Undergraduate Seminar 1999-2000: co-organizer of Dept Seminar Series. Weekly PHOL 468 (Membrane Physiology) 2003, Spring: 2 x 1.5 hr lecture / discussion on “Na+ coupled transporters” (4.5 hr total) 2004, Spring: 5 x 1.5 hr lecture / discussion on “Na+ coupled transporters” (7.5 hr total) Na+ coupled Cotransporters, other Cotransporters, Exchangers, water transport (thermodynamics & kinetics) 2004, Fall: 5 x 1.5 hr lecture / discussion on “Transporters,” (7.5 hr total) Na+ coupled Cotransporters, other Cotransporters, Exchangers, Cellular Ion Homeostasis, water transport (thermodynamics & kinetics) 2005, Fall: 3 x 1.5 hr lecture / discussion on “Transporters,” (4.5 hr total) Cotransporters, Exchangers, and Regulation of cellular