CKMT2 (Creatine Kinase S-Type, Mitochondrial, Basic- Type Mitochondrial Creatine Kinase, Mib-CK, Sarcomeric Mitochondrial Creatine Kinase, S-MtCK, CKMT2, sMtCK) Catalog number 221721 Supplier United States Biological CKMT2 belongs to the creatine kinase isoenzyme family, and is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It exists as two isoenzymes, sarcomeric CKMT2 and ubiquitous CKMT2, which are encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. Applications Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Immunogen Synthetic peptide corresponding to amino acids 230-280 of Human CKMT2. Formulation Supplied as a liquid PBS, 15mM sodium azide, pH 7.2. Purity Purified by immunoaffinity chromatography. Specificity Recognizes endogenous levels of CKMT2. Species Crossreactivity: Human, mouse, rat Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications IF IHC WB Crossreactivity Hu Mo Rt Storage -20°C MW 45 Powered by TCPDF (www.tcpdf.org).