The following supplement accompanies the article Unique and highly variable bacterial communities inhabiting the surface microlayer of an oligotrophic lake Mylène Hugoni, Agnès Vellet, Didier Debroas* *Corresponding author: [email protected] Aquatic Microbial Ecology 79:115–125 (2017) Table S1. Phylogenetic affiliation of the surface micro-layer (SML) specific OTUs, the epilimnion (E) specific OTUs and shared to both layers. OTUs number Taxonomic Affiliation Surface microlayer Epilimnion Shared Acidobacteria;Acidobacteria;Acidobacteriales;Acidobacteriaceae (Subgroup 1);Granulicella; 2 0 0 Acidobacteria;Acidobacteria;Acidobacteriales;Acidobacteriaceae (Subgroup 1);uncultured; 1 0 0 Acidobacteria;Acidobacteria;JG37-AG-116; 24 0 0 Acidobacteria;Acidobacteria;Subgroup 13; 0 1 0 Acidobacteria;Acidobacteria;Subgroup 3;Family Incertae Sedis;Bryobacter; 0 0 1 Acidobacteria;Acidobacteria;Subgroup 3;SJA-149; 0 1 1 Acidobacteria;Acidobacteria;Subgroup 4;RB41; 0 1 0 Acidobacteria;Acidobacteria;Subgroup 6; 0 0 1 Actinobacteria;AcI;AcI-A; 0 4 13 Actinobacteria;AcI;AcI-A;AcI-A3; 0 4 0 Actinobacteria;AcI;AcI-A;AcI-A5; 3 1 1 Actinobacteria;AcI;AcI-A;AcI-A7; 0 1 0 Actinobacteria;AcI;AcI-B;AcI-B1; 0 8 1 Actinobacteria;AcI;AcI-B;AcI-B2; 0 7 0 Actinobacteria;Acidimicrobia;Acidimicrobiales;Acidimicrobiaceae;CL500-29 marine group; 7 34 11 Actinobacteria;Acidimicrobia;Acidimicrobiales;Family Incertae Sedis;Candidatus Microthrix; 0 1 0 Actinobacteria;Acidimicrobia;Acidimicrobiales;uncultured; 0 4 1 Actinobacteria;AcIV; 0 2 0 Actinobacteria;AcIV;Iluma-A2; 0 10 0 1 OTUs number Taxonomic Affiliation Surface microlayer Epilimnion Shared Actinobacteria;AcIV;Iluma-B2; 0 1 0 Actinobacteria;AcTH1-A1; 0 4 2 Actinobacteria;Actinobacteria;Corynebacteriales;Corynebacteriaceae;Corynebacterium; 2 0 0 Actinobacteria;Actinobacteria;Corynebacteriales;Mycobacteriaceae;Mycobacterium; 0 0 1 Actinobacteria;Actinobacteria;Corynebacteriales;Nocardiaceae;Gordonia; 0 1 0 Actinobacteria;Actinobacteria;Corynebacteriales;Nocardiaceae;Nocardia; 1 4 0 Actinobacteria;Actinobacteria;Corynebacteriales;Nocardiaceae;Rhodococcus; 0 2 2 Actinobacteria;Actinobacteria;Corynebacteriales;Nocardiaceae;Williamsia; 0 1 0 Actinobacteria;Actinobacteria;Frankiales;Frankiaceae;Frankia; 2 0 0 Actinobacteria;Actinobacteria;Frankiales;Sporichthyaceae; 0 9 3 Actinobacteria;Actinobacteria;Frankiales;Sporichthyaceae;Candidatus Planktophila; 0 5 3 Actinobacteria;Actinobacteria;Frankiales;Sporichthyaceae;hgcI clade; 3 44 23 Actinobacteria;Actinobacteria;Micrococcales;Dermabacteraceae;Brachybacterium; 1 0 0 Actinobacteria;Actinobacteria;Micrococcales;Microbacteriaceae;Alpinimonas; 0 0 3 Actinobacteria;Actinobacteria;Micrococcales;Microbacteriaceae;Candidatus Limnoluna; 0 1 0 Actinobacteria;Actinobacteria;Micrococcales;Microbacteriaceae;Candidatus Planktoluna; 0 2 1 Actinobacteria;Actinobacteria;Micrococcales;Microbacteriaceae;Microbacterium; 0 1 0 Actinobacteria;Actinobacteria;Micrococcales;Microbacteriaceae;ML602J-51; 0 1 0 Actinobacteria;Actinobacteria;Micrococcales;Microbacteriaceae;MWH-Ta3; 0 1 1 Actinobacteria;Actinobacteria;Micrococcales;Micrococcaceae;Arthrobacter; 0 3 0 Actinobacteria;Actinobacteria;PeM15; 0 1 0 Actinobacteria;Actinobacteria;Propionibacteriales;Nocardioidaceae;Nocardioides; 0 1 0 Actinobacteria;Actinobacteria;Propionibacteriales;Propionibacteriaceae;Propionibacterium; 16 0 0 Actinobacteria;Actinobacteria;Pseudonocardiales;Pseudonocardiaceae;Pseudonocardia; 0 2 0 Actinobacteria;Thermoleophilia;Gaiellales;uncultured; 11 1 0 Actinobacteria;Thermoleophilia;Solirubrobacterales;Patulibacteraceae;Patulibacter; 0 1 0 Armatimonadetes;Armatimonadia;Armatimonadales; 3 1 5 Armatimonadetes;Armatimonadia;Armatimonadales;Armatimonadaceae;Armatimonas; 0 2 0 Armatimonadetes;unclassified Armatimonadetes; 0 0 2 Bacteroidetes;Algor; 0 1 0 Bacteroidetes;Aquir; 0 8 0 Bacteroidetes;Bacteroidia;Bacteroidales;Marinilabiaceae;uncultured; 0 2 0 Bacteroidetes;Bacteroidia;Bacteroidales;Rikenellaceae;vadinBC27 wastewater-sludge group; 0 1 0 Bacteroidetes;Cytophagia;Cytophagales;Cyclobacteriaceae;uncultured; 0 3 4 Bacteroidetes;Cytophagia;Cytophagales;Cytophagaceae;Arcicella; 3 1 4 Bacteroidetes;Cytophagia;Cytophagales;Cytophagaceae;Emticicia; 0 2 0 Bacteroidetes;Cytophagia;Cytophagales;Cytophagaceae;Flexibacter; 1 2 1 2 OTUs number Taxonomic Affiliation Surface microlayer Epilimnion Shared Bacteroidetes;Cytophagia;Cytophagales;Cytophagaceae;Hymenobacter; 2 0 0 Bacteroidetes;Cytophagia;Cytophagales;Cytophagaceae;Runella; 0 1 0 Bacteroidetes;Cytophagia;Cytophagales;Cytophagaceae;Siphonobacter; 0 1 0 Bacteroidetes;Cytophagia;Cytophagales;Flammeovirgaceae;Reichenbachiella; 0 1 2 Bacteroidetes;Cytophagia;Cytophagales;MWH-CFBk5; 0 1 0 Bacteroidetes;Flavo; 0 1 1 Bacteroidetes;Flavobacteria;Flavobacteriales;Blattabacteriaceae;Candidatus Sulcia; 0 10 0 Bacteroidetes;Flavobacteria;Flavobacteriales;Cryomorphaceae;Fluviicola; 0 11 5 Bacteroidetes;Flavobacteria;Flavobacteriales;Cryomorphaceae;uncultured; 0 2 1 Bacteroidetes;Flavobacteria;Flavobacteriales;Flavobacteriaceae;Chryseobacterium; 2 0 0 Bacteroidetes;Flavobacteria;Flavobacteriales;Flavobacteriaceae;Flavobacterium; 5 31 14 Bacteroidetes;Flavobacteria;Flavobacteriales;NS9 marine group; 0 7 2 Bacteroidetes;FukuN47; 0 3 2 Bacteroidetes;PRD18D09; 1 1 10 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Chitinophagaceae;Ferruginibacter; 0 4 0 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Chitinophagaceae;Filimonas; 0 1 0 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Chitinophagaceae;Hydrotalea; 1 5 5 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Chitinophagaceae;Sediminibacterium; 0 15 12 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Chitinophagaceae;uncultured; 2 2 5 Bacteroidetes;Sphingobacteria;Sphingobacteriales;env.OPS 17; 4 5 4 Bacteroidetes;Sphingobacteria;Sphingobacteriales;LiUU-11-161; 0 7 3 Bacteroidetes;Sphingobacteria;Sphingobacteriales;NS11-12 marine group; 13 16 1 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Saprospiraceae;Saprospira; 0 2 0 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Saprospiraceae;uncultured; 0 1 0 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Sphingobacteriaceae;Mucilaginibacter; 1 0 0 Bacteroidetes;Sphingobacteria;Sphingobacteriales;Sphingobacteriaceae;Pedobacter; 0 2 0 Bacteroidetes;vadinHA17; 1 1 0 BD1-5;unclassified BD1-5; 0 1 0 Caldiserica;Caldisericia;Caldisericales;Caldisericaceae;Caldisericum; 0 1 0 Candidate division OD1;unclassified Candidate division OD1; 2 6 0 Candidate division OP11;unclassified Candidate division OP11; 1 2 0 Candidate division TM7;unclassified Candidate division TM7; 0 2 1 Chlamydiae;Chlamydiae;Chlamydiales;Simkaniaceae;Candidatus Rhabdochlamydia; 0 1 0 Chlorobi;Chlorobia;Chlorobiales;OPB56; 0 3 0 Chlorobi;Ignavibacteria;Ignavibacteriales;PHOS-HE36; 1 1 0 Chlorobi;Ignavibacteria;Ignavibacteriales;SR-FBR-L83; 0 1 0 Chloroflexi;Anaerolineae;Anaerolineales;Anaerolineaceae;uncultured; 0 1 0 3 OTUs number Taxonomic Affiliation Surface microlayer Epilimnion Shared Chloroflexi;Caldilineae;Caldilineales;Caldilineaceae;uncultured; 0 2 0 Chloroflexi;Chloroflexia;Chloroflexales;Roseiflexaceae;Roseiflexus; 0 6 0 Chloroflexi;Dehalococcoidia;GIF9; 1 4 0 Chloroflexi;P2-11E; 0 1 0 Cyanobacteria;Chloroplast; 12 48 16 Cyanobacteria;Cyanobacteria;SubsectionI;FamilyI;uncultured; 0 4 0 Cyanobacteria;Cyanobacteria;SubsectionIII;FamilyI;Limnothrix; 0 3 0 Cyanobacteria;Cyanobacteria;SubsectionIII;FamilyI;uncultured; 1 0 0 Cyanobacteria;Cyanobacteria;SubsectionIV;FamilyI; 48 0 16 Deinococcus-Thermus;Deinococci;Deinococcales;Trueperaceae;Truepera; 0 1 0 Fibrobacteres;Fibrobacteria;Fibrobacterales;Fibrobacteraceae;uncultured; 0 1 0 Firmicutes;Bacilli;Bacillales;Bacillaceae;Bacillus; 0 1 0 Firmicutes;Bacilli;Bacillales;Paenibacillaceae; 0 1 0 Firmicutes;Bacilli;Bacillales;Staphylococcaceae;Staphylococcus; 5 0 0 Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Lactococcus; 0 1 0 Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus; 1 0 0 Firmicutes;Clostridia;Clostridiales;Caldicoprobacteraceae;Caldicoprobacter; 1 0 0 Firmicutes;Clostridia;Clostridiales;Family XI Incertae Sedis;Anaerococcus; 1 0 0 Firmicutes;Clostridia;Clostridiales;Family XII Incertae Sedis;Fusibacter; 1 0 0 Firmicutes;Clostridia;Clostridiales;Family XIV Incertae Sedis;Anaerobranca; 0 1 0 Firmicutes;Clostridia;Clostridiales;Family XVII Incertae Sedis;uncultured; 3 0 0 Firmicutes;Clostridia;Clostridiales;Lachnospiraceae;Incertae Sedis; 21 0 0 Firmicutes;Clostridia;Clostridiales;Lachnospiraceae;uncultured; 1 0 0 Firmicutes;Clostridia;Clostridiales;Ruminococcaceae;Incertae Sedis; 11 1 0 Firmicutes;Clostridia;Clostridiales;Ruminococcaceae;uncultured; 3 2 0 Firmicutes;Clostridia;Clostridiales;Syntrophomonadaceae;uncultured; 4 0 0 Firmicutes;Clostridia;Thermoanaerobacterales;Thermodesulfobiaceae;Thermodesulfobium; 1 0 0 Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas; 2 1 1 Firmicutes;OPB54; 2 0 0 Gemmatimonadetes;Gemmatimonadetes;Gemmatimonadales;Gemmatimonadaceae;Gemmatimonas; 0 2 0 Lentisphaerae;Lentisphaeria; 0 1 0 Lentisphaerae;Lentisphaeria;WCHB1-41; 0 3 0 Planctomycetes;Phycisphaerae;Phycisphaerales;Phycisphaeraceae;AKYG587; 0 1 0 Planctomycetes;Phycisphaerae;Phycisphaerales;Phycisphaeraceae;FS140-16B-02 marine group; 0 8 0 Planctomycetes;Phycisphaerae;Phycisphaerales;Phycisphaeraceae;SM1A02; 2 0 1 Planctomycetes;Planctomycetacia;Planctomycetales;Planctomycetaceae;Pirellula; 0 6 0 Planctomycetes;Planctomycetacia;Planctomycetales;Planctomycetaceae;Planctomyces; 1 3 0 4 OTUs number Taxonomic