HOW POTENT IS YOUR CANNABIS? 3 Quick Methods for Cannabinoid Separations As Cannabis is increasingly used for medical applications, there is rising demand for quick and reliable cannabis testing methods. Cannabis contains over 100 cannabinoid compounds, some of which have potential therapeutic uses. Consequently, Cannabis cultivators are under pressure to ensure cannabinoid identity, purity, and concentration. Cannabinoids can be analyzed using multiple techniques, with HPLC being the most common. The following are three methods for cannabinoid separations that provide quick and reliable identification and quantification. METHOD 1 METHOD 2 METHOD 3 No. Cannabinoids Separated 10 14 14 Time under 4 minutes 8 minutes 6 minutes Conditions Isocratic Gradient Column Ascentis Express C18, 10 cm x 4.6 mm, 2.7 μm Ascentis Express C18, 2.7 μm, 3 x 150 mm (53816-U) Ascentis Express C18, 2.7 μm, 3 x 150 mm (53816-U) Mobile Phase A 0.1% formic acid in water 0.1% formic acid in water 0.1% formic acid in water Mobile Phase B 0.085% formic acid in acetonitrile 0.085% formic acid in acetonitrile 0.085% formic acid in acetonitrile Gradient 77 to 85% B in 4 min 70 to 88% B in 6 min Mixing Ratio 25:75, A:B Flow Rate 1.5 mL/min 1 mL/min 1 mL/min Initial Pressure 2860 psi 5100 psi 5100 psi Column Temp 38 °C 30 °C 30 °C Detector UV at 220nm UV at 220 nm UV at 220 nm Injection 1.3 μL 0.6 μL 0.6 μL Sample 0.5 μg/μL each 0.04 g/L each in 50:50, water:acetonitrile 0.04 g/L each in 50:50, water:acetonitrile FEATURED 1. Cannabidivarin (CBDV) 7. Δ9-tetrahydrocannabinol 1. Cannabidivarinic acid (CBDVA) 9. delta-9- Tetrahydrocannabinol 1. Cannabidivarinic acid (CBDVA) 9. delta-9- Tetrahydrocannabinol 2. Cannabidiolic acid (CBDA) (Δ9-THC) 2. Cannabidvarin (CBDV) (Δ9-THC) 2. Cannabidvarin (CBDV) (Δ9-THC) PRODUCT 3. Cannabigerol (CBG) 8. Δ8-tetrahydrocannabinol 3. Cannabidiolic acid (CBDA) 10. delta-8-Tetrahydrocannabinol 3. Cannabidiolic acid (CBDA) 10. delta-8-Tetrahydrocannabinol 4. Cannabidiol (CBD) (Δ8-THC) 4. Cannabigerolic acid (CBGA) (Δ8-THC) 4. Cannabigerolic acid (CBGA) (Δ8-THC) Industry leading 5. Tetrahydrocannabivarin 9. Cannabichromene (CBC) 5. Cannabigerol (CBG) 11. Cannabicyclol (CBL) 5. Cannabigerol (CBG) 11. Cannabicyclol (CBL) (THCV) 10. Δ9-tetrahydrocannabinolic 6. Cannabidiol (CBD) 12. Cannabichromene (CBC) 6. Cannabidiol (CBD) 12. Cannabichromene (CBC) ® Cerilliant cannabinoid 6. Cannabinol (CBN) acid A (THCA) 7. Tetrahydrocannabivarin 13. delta-9-Tetrahydrocannabinolic 7. Tetrahydrocannabivarin 13. delta-9-Tetrahydrocannabinolic (THCV) acid A (THCA) (THCV) acid A (THCA) Certified Reference 8. Cannabinol (CBN) 14. Cannabichromenic acid (CBCA) 8. Cannabinol (CBN) 14. Cannabichromenic acid (CBCA) Materials that provide reproducible results for potency testing. Isocratic Elution Gradient Elution When the composition of the mobile phase remains When the ratio of polar to non-polar compounds in constant throughout the HPLC separation. the mobile phase changes during the sample run..