UK Standards for Microbiology Investigations2014 Identification of Vibrio and Aeromonas species NOVEMBER 10 - OCTOBER 17 BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 19 | Issue no: dzc+ | Issue date: dd.mm.yy <tab+enter> | Page: 1 of 31 © Crown copyright 2014 Identification of Vibrio and Aeromonas species Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website http://www.hpa.org.uk/SMI/Partnerships. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see http://www.hpa.org.uk/SMI/WorkingGroups). The contributions of many individuals in clinical, specialist and reference laboratories2014 who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: NOVEMBER 10 Standards Unit - Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ OCTOBER E-mail: [email protected] 17 Website: http://www.hpa.org.uk/SMI UK Standards for Microbiology Investigations are produced in association with: BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Bacteriology – Identification | ID 19 | Issue no: dzc+ | Issue date: dd.mm.yy <tab+enter> | Page: 2 of 31 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Vibrio and Aeromonas species Contents ACKNOWLEDGMENTS .......................................................................................................... 2 AMENDMENT TABLE ............................................................................................................. 4 UK STANDARDS FOR MICROBIOLOGY INVESTIGATIONS: SCOPE AND PURPOSE ....... 7 SCOPE OF DOCUMENT ....................................................................................................... 10 INTRODUCTION ................................................................................................................... 10 2014 TECHNICAL INFORMATION/LIMITATIONS ......................................................................... 13 1 SAFETY CONSIDERATIONS .................................................................................... 15 2 TARGET ORGANISMS ..............................................................................................NOVEMBER 15 10 - 3 IDENTIFICATION ....................................................................................................... 16 4A IDENTIFICATION OF VIBRIO SPECIES .................................................................... 23 4B IDENTIFICATION OF AEROMONAS SPECIES ........................................................OCTOBER 24 17 5 REPORTING .............................................................................................................. 25 6 REFERRALS .............................................................................................................. 26 BETWEEN 7 NOTIFICATION TO PHE OR EQUIVALENT IN THE DEVOLVED ON ADMINISTRATIONS .................................................................................................. 26 REFERENCES ...................................................................................................................... 28 CONSULTED WAS DOCUMENT THIS - DRAFT Bacteriology – Identification | ID 19 | Issue no: dzc+ | Issue date: dd.mm.yy <tab+enter> | Page: 3 of 31 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Vibrio and Aeromonas species Amendment Table Each SMI method has an individual record of amendments. The current amendments are listed on this page. The amendment history is available from [email protected]. New or revised documents should be controlled within the laboratory in accordance with the local quality management system. Amendment No/Date. 5/dd.mm.yy <tab+enter> 2014 Issue no. discarded. 2.2 Insert Issue no. #.# <tab+enter> Section(s) involved Amendment NOVEMBER 10 The scope has been updated to include- Scope of document. Aeromonas species. The taxonomy of Vibrio species has been updated. Aeromonas species haveOCTOBER been added into this document. 17 Introduction. More information has been added to the Characteristics section. The medically important species ofBETWEEN Aeromonas and Vibrio are mentioned. SectionON on Principles of Identification has been updated to include the MALDI-TOF. Addition of information regarding serology, oxidase Technical test, Gram stain, commercial identification systems Information/Limitations. CONSULTEDand differentiation between Aeromonas and Vibrio species. WAS This section has been updated on the handling of Safety considerations. Aeromonas and Vibrio species as well as laboratory acquired infections. DOCUMENT The section on the Target organisms has been Target Organisms. updated and presented clearly for both organisms. THIS - Updates have been done on 3.2, 3.3 and 3.4 to reflect standards in practice. DRAFT Section 3.4.2, 3.4.3 and 3.4.4 has been updated to Identification. include Commercial Identification Systems, MALDI-TOF MS and NAATs with references. Subsection 3.5 has been updated to include the Rapid Molecular Methods. Bacteriology – Identification | ID 19 | Issue no: dzc+ | Issue date: dd.mm.yy <tab+enter> | Page: 4 of 31 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Vibrio and Aeromonas species Modification of flowchart for identification of Vibrio species has been done for easy guidance. A new Identification Flowchart. flowchart for identification of Aeromonas species has also been done. Subsections 5.3, 5.5 and 5.6 have been updated Reporting. to reflect the information required on reporting practice. The addresses of the reference laboratories have Referral. been updated. 2014 Document presented in a new format. Document has been transferred to a new template to reflect the Health Protection Agency’s transition to Public Health England. NOVEMBER 10 - Front page has been redesigned. Whole document. Status page has been renamed as Scope and Purpose and updated asOCTOBER appropriate. 17 Professional body logos have been reviewed and updated. Standard safetyBETWEEN and notification references have been reviewedON and updated. References. Some references updated. Amendment No/Date. CONSULTED4/11.03.14 Issue no. discarded. WAS 2.1 Insert Issue no. 2.2 Section(s) involved Amendment DOCUMENT Document has been transferred to a new template THIS to reflect the Health Protection Agency’s transition - to Public Health England. Front page has been redesigned. DRAFT Status page has been renamed as Scope and Whole document. Purpose and updated as appropriate. Professional body logos have been reviewed and updated. Standard safety and notification references have been reviewed and updated. Bacteriology – Identification | ID 19 | Issue no: dzc+ | Issue date: dd.mm.yy <tab+enter> | Page: 5 of 31 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Vibrio and Aeromonas species Scientific content remains unchanged. 2014 NOVEMBER 10 - OCTOBER 17 BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Bacteriology – Identification | ID 19 | Issue no: dzc+ | Issue date: dd.mm.yy <tab+enter> | Page: 6 of 31 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Vibrio and Aeromonas species # UK Standards for Microbiology Investigations : Scope and Purpose Users of SMIs • SMIs are primarily intended as a general resource for practising professionals operating in the field of laboratory medicine and infection specialties in the UK • SMIs provide clinicians with information about the available test repertoire and the standard of laboratory services they should expect for the investigation of infection in their patients, as well as providing information that aids the 2014 electronic ordering of appropriate tests • SMIs provide commissioners of healthcare services with the appropriateness and standard of microbiology investigations they should be seeking as part of the clinical and public health care package for their population NOVEMBER 10 Background to SMIs - SMIs comprise a collection of recommended algorithms and procedures covering all stages of the investigative process in microbiology from the pre-analytical (clinical syndrome) stage to the analytical (laboratory testing) and OCTOBERpost analy tical (result interpretation and reporting) stages. 17 Syndromic algorithms are supported by more detailed documents containing advice on the investigation of specific diseases and infections. Guidance notes cover the clinical background, differential diagnosis, and appropriate investigation of particular clinical conditions. Quality guidance notes describeBETWEEN laboratory processes which underpin quality, for example assay validation.ON Standardisation of the diagnostic process through the application of SMIs helps to assure the equivalence of investigation strategies in different laboratories across the UK and is essential for public health surveillance, research and development