Mohammad Azizur Rahman et al., IJTCM, 2020, 5:29 Research Article IJTCM (2020), 5:29 International Journal of Traditional and Complementary Medicine ( (ISSN:2474-1361) Proximate Analysis, Phtochemical Screening and Antioxidant Activity Of Different Strains of Auricularia auricula-judae (Ear Mushroom) Mohammad Azizur Rahman*, Abdullah Al Masud, Nilufar Yasmin Lira, Salman Shakil Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh. ABSTRACT In this study, proximate analysis, phytochemical screening and *Correspondence to Author: antioxidant activity of two strains of ear mushroom Auricularia Mohammad Azizur Rahman auricula (arbitrarily named strain 3 and 5) and their mix, cultivated Department of Biochemistry and in Bangladesh National Mushroom Development Institute, Molecular Biology, Jahangirnagar have been determined. Protein content per 100 gm of strain University, Savar, Dhaka 1342, 5, 7 and mix had been found to be 298.69mg, 278.85mg and Bangladesh. 286.19mg, respectively. Lipid content estimated were 2.43gm, 1.96 gm and 2.4gm, respectively, while that of ash were 4.42 gm, How to cite this article: 6.11gm and 3.93gm, respectively. A. auricula strain 7 contained Mohammad Azizur Rahman*, highest amounts of total phenol, total flavonoid, ascorbic acid Abdullah Al Masud, Nilufar Yasmin and reducing sugar than the others. Among the three strains Lira, Salman Shakil.Proximate evaluated in the present study, A. auricula 7 contained highest Analysis, Phtochemical Screening nutritional and medicinal components. Thus, A. auricula 7 might and Antioxidant Activity Of Different be an ideal food supplement to the consumers. Strains of Auricularia auricula-judae (Ear Mushroom). International Keywords: Anti-oxidant; Auricularia auricula; Phytochemical Journal of Traditional and analysis; Proximate composition. Complementary Medicine 2020, 5:29. eSciPub LLC, Houston, TX USA. Website: https://escipub.com/ IJTCM: https://escipub.com/international-journal-of-traditional-and-complementary-medicine/ 1 Mohammad Azizur Rahman et al., IJTCM, 2020, 5:29 Introduction nutritional and medicinal values of A. auricula so Auricularia auricular-judae is the mushroom of that the consumer would be aware of its auricularia genus. These mushrooms are nutritional and medicinal values and consider commonly called ear mushroom due to their ear this mushroom among the functional food items. like appearance.Their fruting body is brown in Materials and methods coloration and grows upon wood which is Mushroom collection and preparation especially damp and elder. A. auricula (common Fruiting bodies of A. auricula were collected from names: mu-er, wood ear), are Bangladesh national mushroom development heterobasidiomycetes, in the order institute and cut into small pieces. Small pieces Auriculariales and the family Auriculariaceae. A. were dried under the sun followed by hot air auricula was the first artificially cultivated oven at the temperature 55°C until proper drying. mushroom in China. Auricularia mushrooms are After drying, the dried chips were ground into among the top four most important cultivated coarse powders using blender having high mushrooms in the world, grown mainly in China capacity grinding power. Then the powder were and Southeast Asia, with a world annual stored in air tight container with necessary production of 420,000 tons (Luo,et al, markings for identification and kept in cool, dark 2004).Their unique jelly texture and horizontally and dry place for further investigation. Hot water septated basidium make them significantly extract (HWE) of A. auricula was prepared different in taste and morphological characters following the method of Rahman et al. (2016) [4]. from other cultivated mushrooms, such as Proximate analysis Agaricus bisporus, Lentinula edodes, Pleurotus Following the procedure established by the spp., and others. Besides their taste and Association of Official Analytical Chemists nutrition, they also have medical functions, such (AOAC), the analyses were performed [5]. as anti-tumour, immuno-stimulating, Analyses included the determination of crude hypolipidaemic and hypocholesterolaemic protein, crude fat, ash, crude fiber, moisture and effects (Ukai et al. 1983; Cheung 1996; Song et carbohydrate. The percentage of all the fractions al. 1998; Yang et al. 2002). They can grow well (crude protein, crude fat and ash) were added on wide ranges of agricultural and industrial together and subtracted from 100 to obtain the organic wastes. Recently, a nature-imitated total carbohydrate percentage. cultivation method has been developed in China Antioxidant studies by cultivating Auricularia mushrooms in corn Qualitative screening for antioxidant activity fields, and thereafter, using the spent compost Determination of phenols (Ferric chloride as organic fertilizer and soil conditioner (Yan et test) al. 1995). Following the method of Soloway and Wilen Though the usage of mushrooms is being (1952) [6], ferric chloride test was performed to increased day by day for their significant role in assess the phenolics in the HWE of A. auricula. human health, nutrition and disease, in Determination of flavonoids (Alkaline Bangladesh, mushrooms are considered as reagent test) important food items. People in Bangladesh are still not very aware of nutritional and medicinal Following the method of Rahman et al. (2017) importance of mushrooms. Climatic condition of [7], Alkaline reagent test was performed to Bangladesh favors the easy cultivation of ear assess the flavonoids in the HWE of A. auricula. mushroom, A. auricula. Yet, there is scarcity of Determination of ascorbic acid interest of common mass in cultivating and Following the method of Schmall and Pifer including this mushroom in daily diet. Thus, the (1953) [8], HWE of A. auricula was assessed for present study has been aimed at determining the the presence of ascorbic acid. IJTCM: https://escipub.com/international-journal-of-traditional-and-complementary-medicine/ 2 Mohammad Azizur Rahman et al., IJTCM, 2020, 5:29 Quantitative estimation of phytochemical Following the modified method of Nelson- constituents Somogyi (1944) [13], content of reducing sugar Total phenolics content (TPC) assay in the HWE of A. auricula was performed. Following the modified method of Singleton et Antioxidant assay al. (1999) [9], content of total phenolics in the Ferric reducing antioxidant power (FRAP) assay HWE of A. auricula was performed. Following the modified method of Benzie and Total flavonoid content (TFC) assay Strain (1996) [14], ferric reducing antioxidant Following the modified method of Chang et al. power (FRAP) assay of the HWE of A. auricula (2002) [10], content of total flavonoids in the was performed. HWE of A. auricula was performed. DPPH (1,1-diphenyl-2-picryl-hydrazyl) Free Ascorbic acid content assay radical scavenging activity assay Following the modified method of Omaye et al. The ability of the HWE of A. auricula to scavenge (1979) [11], content of ascorbic acid in the HWE 2,2diphenyl-1-picrylhydrazyl (DPPH) free of A. auricula was performed. radicals was determined according to the method Brand-williams (1995) [15] with little Total protein content assay modification. Following the modified method of Lowry et al. Results and Discussion (1951) [12], content of total protein in the HWE Proximate analysis of A. auricula of A. auricula was performed. Reducing sugar content assay TABLE 1: Proximate analysis of A. auricula strains (%dry weight) Proximate composition A. auricula 3 A. auricula 5 A. auricula mix Crude lipid 1.6±0.124 1.9±0.188 1.2±0.294 Crude protein 18.75±0.12 29.90±0.14 27.90±0.18 Fiber 22.8±0.106 19.14±0.260 21.8±0.241 Carbohydrate 51.65±0.13 37.18±0.15 38.91±0.17 Ash 3.77±0.0.121 3.69±0.249 3.96±0.187 Moisture 9.04±0.43 6.34±0.38 6.81±0.33 Results are expressed as mean±SEM. The lipid content of 100 gm of dried A. auricula auricula mix were found about 22.8gm, 19.14 gm 3, A. auricula 5, A. auricula mix were found about and 21.8gm respectively. 1.6gm, 1.9 gm and 1.2 gm respectively. The Phytochemical analysis of A. auricula moisture content of A. auricula 3, A. auricula 5, The maximum TPC was obtained in ethanolic A. A. auricula mix were found about 9.04%,6.34% auricula 5 extract (24.89 mg GAE/100g extract) and 6.81%respectively. The ash content of 100 at a concentration of 0.5 mg/mL. At the same gm of dried A. auricula 3, A. auricula 5, A. concentration, the minimum TPC was observed auricula mix were found about 3.77gm,3.69 gm in ethanolic A. auricula mix extract (20.45 mg and 3.96gm respectively. The fibre content of GAE/100g extract). This report agrees to the one 100 gm of dried A. auricula 3, A. auricula 5, A. given by (Al-Juhaimi, 2014) that A. auricula 5 IJTCM: https://escipub.com/international-journal-of-traditional-and-complementary-medicine/ 3 Mohammad Azizur Rahman et al., IJTCM, 2020, 5:29 exhibit more phenol content than A. auricula 3 application in food products than butylated and A. auricula mix extract. The health benefits hydroxytoluene and butylated hydroxyanisole of polyphenolics are primarily derived from their which are artificial compounds with antioxidant antioxidant potentials because the radicals properties (Ghafoor et al., 2011). The maximum produced after hydrogen or electron donation TPC was obtained in ethanolic Au 5 extract are resonance stabilized and thus relatively (24.89mg GAE/100g extract) at a concentration stable (Denre, 2014). The use of phenolics is of 500 µg/mL. At the same concentration, the also reported for effective secretion of dopkine, minimum TPC was observed in ethanolic Au-mix lowering and preventing obesity and prevention extract (20.45 mg GAE/100g extract).This report of oxidative stress (Dalar et al., 2014). These agrees to those of Al-Juhaimi (2014) who polyphenolic compounds from natural sources reported that A. auricula 5 exhibit more phenol are recommendable as natural food additives content than A. auricula 3 A.