View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by DigitalCommons@University of Nebraska University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Faculty Publications from the Harold W. Manter Laboratory of Parasitology Parasitology, Harold W. Manter Laboratory of 4-1996 Two New Species of Tetraphyllidean Cestodes in Himantura pacifica (Chondrichthyes: Myliobatiformes: Dasyatididae) from the Northwest Coast of Costa Rica Fernando Marques University of Toronto Daniel R. Brooks University of Toronto, [email protected] Helena Molina Ureña Universidad de Costa Rica Follow this and additional works at: https://digitalcommons.unl.edu/parasitologyfacpubs Part of the Parasitology Commons Marques, Fernando; Brooks, Daniel R.; and Molina Ureña, Helena, "Two New Species of Tetraphyllidean Cestodes in Himantura pacifica (Chondrichthyes: Myliobatiformes: Dasyatididae) from the Northwest Coast of Costa Rica" (1996). Faculty Publications from the Harold W. Manter Laboratory of Parasitology. 273. https://digitalcommons.unl.edu/parasitologyfacpubs/273 This Article is brought to you for free and open access by the Parasitology, Harold W. Manter Laboratory of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Faculty Publications from the Harold W. Manter Laboratory of Parasitology by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. J. Parasitol., 82(2), 1996, p. 302-306 ? American Society of Parasitologists 1996 TWO NEW SPECIES OF TETRAPHYLLIDEANCESTODES IN HIMANTURAPACIFICA (CHONDRICHTHYES: MYLIOBATIFORMES: DASYATIDIDAE)FROM THE NORTHWESTCOAST OF COSTA RICA Fernando Marques, Daniel R. Brooks, and Helena Molina Urefia* Department of Zoology, Universityof Toronto, Toronto, Ontario,Canada M5S 1A1 ABSTRACT: Two new species of tetraphyllideancestodes inhabitingHimantura pacifica from the northwestcoast of Costa Rica are apparentlymost closely relatedto species inhabitingHimantura schmardae from the Atlantic coast of Colombia.Acantho- bothroidespacificus n. sp. differsfrom Acanthobothroides thorsoni, the only otherspecies in the genus,primarily by havingsmaller lateral(98-123 ,umrather than 168-198 ,tm long) and medial (handles 92-116 Amrather than 162-168 ,umlong) hooks, and more testes (up to 125 ratherthan up to 97) per proglottis.The genericdiagnosis of Acanthobothroidesis modified to include the presenceof a small inner prong on the large medial bothridialhooks. Rhinebothriumgeminum n. sp. and Rhinebothrium magniphallumare the only 2 species in the genus possessingunusually long cirrussacs, extendingfrom the genitalatrium all the way to the ovarianisthmus; R. geminumaverages 12 (12-14) loculi perbothridium and 11 (9-12) testes,whereas R. magniphallum averages17 (16-18) loculi and 14 (10-16) testes per proglottis. The presence of sister species on each side of the Panama DESCRIPTION isthmus has been for sea reported octopods, urchins, pinnother- Acanthobothroides pacificus n. sp. id crabs of the genus Dissodactylus, and atherinid fishes (Voight, (Fig.1A-D) others. 1988; Lessios, 1979; Griffith, 1987; White, 1986) among Description(based on 2 completeand 2 incompletespecimens): Stro- However, studies referring to pairs of species on both side of bila craspedote,anapolytic, up to 65.3-122 mm long; composed of up the isthmus are scarce for helminths, restricted to Manter's (1940) to 409 proglottides.Scolex 719-954 (n = 2) long by 935-1,078 (n = 2) list of species pairs of digenetic trematodes inhabiting marine wide. Bothridia 630-814 (716 ? 67; n = 7) long by 324-445 (383 ? n = 394-464 ? n = fishes. Brooks (1977) described 6 new species of tetraphyllidean 38; 7) wide;anterior loculus (422 30; 7) long, middle loculus 140-197 (169 ? 19; n = 7), posteriorloculus 89-159 (124 ? cestodes inhabiting a species of marine stingray, Himantura 22; n = 7). Ratio of loculi lengths 1:0.4:0.3. Bothridialapical suckers schmardae (Werner), from the Caribbean coast of Colombia. 63-79 (70 ? 7; n = 6) in diameter,pads 173-198 (182 ? 12; n = 5). The present paper describes 2 new species of tetraphyllideans Medial bothridialhooks 100-131 (120 + 10; n = 6) long; handle 92- = inhabiting the putative sister species of H. schmardae, Himan- 116 (106 ? 9; n = 7) long, inner prong 41-54 (47 ? 4, n 7), outer prong66-82 (77 + 7, n = 7). Lateralbothridial hooks 98-123 (109 ? tura (Beebe and Tee-Van), from the Pacific coast of pacifica 11; n = 7) long; handle 64-79 (71 ? 4; n = 7), inner prong 43-57 (51 Costa Rica. + 5; n = 7), outerprong 59-71 (64 ? 4; n = 7). Cephalicpeduncle 5.8- 26.8 (16.0 ? 9.0; n = 4) mm long. Immatureproglottides wider than long. Mature proglottides394-604 (488 ? 61; n = 14) long by 1.28- MATERIALSAND METHODS 1.70 (1.53 + 0.15; n = 14) mm wide. Testes 80-125 (104 ? 13; n = 14) in number,43-101 (77 ? 11; n = 100) in diameter, 11-20 (15 ? In June 1992, 1 specimen of Himanturapacifica (Beebe and Tee- 3; n = 14) postporally,23-39 (33 ? 6; n = 14) preporally,46-69 (57 Van) was capturedat PlayaPanama, Guanacaste Province, Costa Rica, + 6; n = 14) antiporally.Cirrus sac near mid-proglottis,337-445 (401 using a beach seine. Wormswere removed from the spiralvalve, killed + 24; n = 41) long by 146-223 (188 + 20; n = 41) wide, contained with hot tap water,transferred immediately to AFA for 24-48 hr, and spined eversiblecirrus. Vagina anterior to cirrussac; vaginal sphincter then stored in 70%ethanol. Specimenswere stained with Mayer'she- a muscular-glandularthickening near genital pore. Proglottidesnot con- matoxylin and mounted in Canadabalsam for examinationas whole taining testes, but with sperm in the vas deferens 0.46-1.37 (0.85 ? mounts. Measurementsare in jzm unless otherwise stated; for some 0.26; n = 17) mm long by 0.67-1.62 (1.15 ? 0.27; n = 17) mm wide. ? 1 traits, rangesare given, followed in parenthesesby mean values Genitalpore 43-53% (46 + 3; n = 19) of proglottislength from anterior standarddeviation and the samplesize (n). Hook measurementsfollow end. Ovary follicular305-420 (373 ? 48; n = 7) long by 922-1,049 the formulaof Euzet (1956) modified as follows: For each parameter, (998 ? 46; n = 7) wide at isthmus. Isthmus 69-176 (115 ? 2; n = 12) mean value ? 1 standarddeviation is given, followed in parentheses long by 66-108 (99 ? 20; n = 12) wide. Vitelline follicles extending by its range.Figures were drawnwith the aid of cameralucida using a entirelength of proglottis,from level of ovarianisthmus to nearanterior BH-2 Olympus microscope.The tetralobednature of the ovaries was end. Uterus not observed. confirmedfrom lateralviews of unmountedspecimens and from whole mounts because sufficientmaterial for sectioning was not available. Taxonomic summary MNHG refersto the Museumof NaturalHistory, Geneva, Switzerland; USNPC refersto the U.S. NationalParasite Collection, Beltsville, Mary- Host: Himanturapacifica (Beebe and Tee-Van). land; UNSMHWML refers to the University of NebraskaState Mu- Site of infection: Spiralvalve. seum, HaroldW. ManterLaboratory, Division of Parasitology,Lincoln, Locality: PlayaPanama, Guanacaste, Costa Rica (10?15'N; 86?00'W). Nebraska,U.S.A. Holotype: MNHG no. INV 20585. Paratypes: MNHG no. INV 20586; UNSMHWMLno. 38742. Etymology: The species is named for the ocean from which it was first described. Remarks Received 10 July 1995;revised 30 October1995; accepted 30 October Acanthobothroideswas proposed by Brooks (1977) for A. thorsoni 1995. Brooks, 1977 collected in the spiral valve of H. schmardaefrom the * Escuelade Biologia,Universidad de CostaRica, SanJose, CostaRica. Atlantic coast of Colombia.Acanthobothroides thorsoni possessed sco- 302 MARQUESET AL.-TWO NEW CESTODESFROM COSTA RICANSTINGRAYS 303 A C A 500 im B 100 gim C 500 im D F D 150 gim E 500 (Jm F 500 gim E FIGURE 1. Acanthobothroides pacificus n. sp. and A. thorsoni. A. Scolex of A. pacificus n. sp. B. Medial and lateral hooks of A. pacificus n. sp. C. Medial hook of A. thorsoni (UNSMHWML no. 20259, paratype). D. Immature proglottis (dorsal lobe of ovary omitted) in A. pacificus n. sp. E. Mature proglottis with testes in A. pacificus n. sp. F. terminal proglottis without testes but with sperm in vas deferens in A. pacificus n. sp. 304 THE JOURNALOF PARASITOLOGY,VOL. 82, NO. 2, APRIL1996 <0'00 --0 /^c^ae_. t ,/.............:..* .-c'"ig^^ M t ! L : : ; ( : - : 9 y- z ; : - ' X . u i ' - ' E; ,? MARQUESET AL.-TWO NEW CESTODES FROM COSTA RICAN STINGRAYS 305 leces comprising4 triloculatebothridia each surmountedby an apical 63-113 (73 + 17;n = 9) wide, containedspined eversible cirrus. Vagina pad and suckerand armed with a pair of hooks. The lateralhook was anterior to cirrus sac; vaginal sphincter a glandularthickening near similar in shape to those exhibitedby species of AcanthobothriumVan genital pore. Genital pore 29-46% (37 + 4; n = 12) of proglottislength Beneden, 1849 and the medial hook describedas havinga singlerobust from anterior end. Ovary V-shaped near posterior end of proglottis, prong, thus distinct from other species of Acanthobothrium.Acantho- lobes fused posteriorly,X-shaped in cross section, 195-378 (263 + 54; bothroidespacificus is highly similar to A. thorsoni.Our specimensare n = 12) long by 60-94 (74 ? 11; n = 9) wide at isthmus. Ovarianlobes smaller (up to 122 mm long) than those of A. thorsoni(up to 400 mm symmetrical,extending anteriorly to 1 ovarianlength posterior to level long) and have fewer segments (approximately400 ratherthan 700). of genital pore, terminatingjust anteriorto posteriormargin of cirrus