6/10/2019 Program for Friday, May 10th GBB 2019: GENES, BRAIN AND BEHAVIOR 2019 SPONSORS PROGRAM AUTHORS KEYWORDS PROGRAM FOR FRIDAY, MAY 10TH Days: next day all days View: session overview talk overview 09:00-16:00 Session 1: Symposium/Workshop Symposia/Workshop presented by the Simons Initiative for the Developing Brain 17:00-18:00 Session 2: Registration Registration 17:50-18:00 Session 3: Presidential Welcome Presidential Welcome CHAIR: Catharine Rankin 18:00-19:00 Session 4: Keynote Special Lecture Dr Carmen Sandi CHAIR: Wim Crusio 18:00 Carmen Sandi Mitochondrial function in the nucleus accumbens links anxiety with stress coping behaviors ABSTRACT. There is important variation in mitochondrial function in the nucleus accumbens that explain anxiety-related differences in social competition. High anxious individuals are highly vulnerable to stress-induced depression. Importantly, high anxious rats display impaired mitochondrial function (respiration, membrane potential, ATP and ROS production) in the nucleus accumbens which is causally implicated in their disadvantage to achieve dominant status. Intra-accumbal infusion of nicotinamide, an amide form of vitamin B3 that boosts mitochondrial function, prevents the development of subordinate status in high anxious rats. Manipulations that modify anxiety levels transiently, such as acute stress or diazepam treatment, modulate social competitiveness and accumbal mitochondrial function. Notably, diazepam treatment when given either systemically or into the ventral tegmental area enhances accumbal mitochondrial respiration https://easychair.org/smart-program/GBB2019/2019-05-10.html 1/2 6/10/2019 Program for Friday, May 10th and ATP production along with increasing dominance behaviors. Furthermore, dominant mice show higher levels in accumbal energy- related metabolites than subordinate mice, as well as increased vulnerability to show social avoidance following social defeat. In subordinates, but not dominants, levels of these metabolites increase following chronic social defeat stress. Our findings have implications for the understanding of the mechanisms involved in individual differences in motivated behavior and vulnerability to stress. Carmen Sandi Brain Mind Institute, Ecole Polytechnique Federal de Lausanne (EPFL), Switzerland 19:00-21:00 Opening Reception Opening Reception Disclaimer | Powered by EasyChair Smart Program https://easychair.org/smart-program/GBB2019/2019-05-10.html 2/2 6/10/2019 Program for Saturday, May 11th GBB 2019: GENES, BRAIN AND BEHAVIOR 2019 SPONSORS PROGRAM AUTHORS KEYWORDS PROGRAM FOR SATURDAY, MAY 11TH Days: previous day next day all days View: session overview talk overview 08:30-09:00 Session 5: Registration Registration 09:00-11:00 Session 6: Symposium 1 Genetics & neurobiology of disordered eating CHAIR: Camron Bryant 09:00 Sarah Mak Elucidation of Secretin in thirst regulation within subfornical organ neurons in the brain ABSTRACT. Precise regulation of plasma osmolality is critical in all terrestrial animals to maintain a constant internal environment and prevent continual water loss. Dysregulation of water homeostasis can result in life-threatening disorders including congestive heart failure and cerebral trauma. Secretin (SCT), a classical gastrointestinal hormone, has been newly described as an osmoregulatory neurohormone that modulates hyperosmolality-induced water drinking behaviour and vasopressin (Vp) release in the hypothalamus. Yet, its molecular mechanism and neural circuitry within the osmoregulatory brain centres is still ripe for exploration. When plasma osmolality changes, subfornical organ (SFO) acts as the first station to detect the changes and transduce them into neuronal signals to other brain regions for corresponding responses. Previous data have shown a wide expression of SCT and its receptor (SCTR) in SFO, and dehydration would increase this expression in SFO. Therefore, we hypothesized that SCT may serve as a neurotransmitter in SFO and modulate water drinking behaviour and Vp release. In this study, we established two specific conditional knockout mice models (SCTSFO-/- and SCTRSFO-/-) to elucidate the role of SCT/SCTR in SFO and investigate its downstream pathway regarding dehydration-induced water intake. We found a marked reduction of water intake in both SCTSFO-/- and SCTRSFO-/- mice (72% drop, https://easychair.org/smart-program/GBB2019/2019-05-11.html 1/28 6/10/2019 Program for Saturday, May 11th p=0.0015; 65% drop, p=0.0387 v.s. sham control) under water-depleted condition. Dehydration- induced cFos expression in SFO was abolished if SCT and SCTR were locally deleted. Thus, current data adds a new piece of information into SCT-mediated osmoregulatory mechanism and may also provide new insights into curing water balance disorders. Sarah O.K. Mak1, Billy K.C. Chow1 1School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China Funding support: This work is supported by Hong Kong Government Research Grant Council Grants GRF HKU17127718 (to B.K.C.C.). The authors declare no conflicts of interest. 09:20 Camron Bryant Forward genetic and gene validation studies of binge-like eating in mice: Sex-sensitive genetic loci, including male-specific linkage with the Tas2r locus ABSTRACT. Eating disorders, including binge eating disorder, bulimia nervosa, and anorexia nervosa are highly lethal, heritable disorders with psychiatric and metabolic components. However, the genetic basis of binge eating is completely unknown. Discovery-based genetic and genomic studies in rodents complement human GWAS and can inform genes, networks, and biological pathways underlying maladaptive feeding. We mapped and validated Cyfip2 as a causal genetic factor underlying binge-like eating (BE) in a Reduced Complexity Cross (RCC) and extended these findings to Cyfip1 where we found complex, sex-specific, parent-of-origin selective effects of Cyfip1 haploinsufficiency on BE. In extending our BE model toward additional genetic crosses, we report robust inbred strain differences in BE and conditioned reward between C57BL/6J and DBA/2J strains in our intermittent, limited access, conditioned place preference paradigm. Candidate locus analysis in F2 mice (N=133) identified a male-specific association of the Tas2r locus on chromosome 6 (133 Mb) with escalated BE. Subsequent genome-wide analysis of BE in the same mice with 3K markers confirmed male-specific genome-wide significant linkage on chromosome 6 (LOD=6.0) and resolved the peak location to 53 cM [113 Mb; 48-59 cM). We also identified a sex- averaged QTL on chromosome 5 [peak=28 cM (53 Mb); LOD=5.2; 21-58 cM), and a female- sensitive QTL on chromosome 8 [LOD=4.1; peak=53 cM (109 Mb); 52-59 cM]. The emerging sex-sensitive genetic architecture of BE in mice https://easychair.org/smart-program/GBB2019/2019-05-11.html 2/28 6/10/2019 Program for Saturday, May 11th has important implications for human GWAS and future therapeutics. Future studies will determine the role of sex hormones and sex chromosomes in the genetic architecture of BE. Camron D. Bryant1, Richard K. Babbs1, Emily Yao1, Melanie M. Chen1, Julia C. Kelliher1, Julia L. Scotellaro1, Kimberly P. Luttik1, Qiu T. Ruan1,2, Megan K. Mulligan3 1.Laboratory of Addiction Genetics, Department of Pharmacology and Experimental Therapeutics and Psychiatry, Boston University School of Medicine 2. Ph.D. Training Program in Biomolecular Pharmacology, Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine 3.Department of Genetics, Genomics, and Informatics, University of Tennessee Health Science Center 09:45 Stephanie Dulawa Dopamine D2 receptor overexpression in the nucleus accumbens core indirect pathway increases activity-based anorexia selectively in female mice ABSTRACT. Anorexia nervosa (AN) is an eating disorder observed predominantly in females that is characterized by hypophagia, weight loss, and compulsive exercise. Increased dopamine D2/D3 receptor binding in the anteroventral striatum has been previously reported in AN. Activity-based anorexia (ABA) refers to the hyperactivity and hypophagia exhibited by rodents exposed simultaneously to running wheels and scheduled feeding, and provides a rodent model for maladaptive behaviors in AN. We virally overexpressed D2Rs on indirect striatal pathway neurons of the nucleus accumbens (NAc) core (D2R-OENacInd), which endogenously express D2Rs, and tested mice of both sexes in the open field and ABA paradigms. D2R-OENacInd did not alter baseline bodyweight, but increased locomotor activity in the open field across both sexes. In addition, D2R-OENacInd mice of both sexes consumed more food than controls when food and running wheels were continuously available during the baseline period of the ABA paradigm. Yet paradoxically, when food was available only 7 hours a day during the restriction phase of ABA, female, but not male, D2R- OENacInd mice showed robust reductions in food intake and survival. Female D2R-OENacInd mice also showed reduced bodyweight, increased wheel running activity, and increased food anticipatory activity. The only effect of D2R- OENacInd on male mice during restriction was a https://easychair.org/smart-program/GBB2019/2019-05-11.html 3/28 6/10/2019 Program for Saturday, May 11th small increase in wheel running, which began late in the restriction period. Our findings indicate that D2R-OENacInd selectively increases ABA behavior in female, but not male, mice. D2R- OENacInd may play a causal role in the maladaptive feeding