REPORT Island of Moen, European Denmark, 12-15 September 2001 Commission for the Control of Foot-and-Mouth Disease Session of the Research Group of the Standing Technical Committee AGA: EUFMD/RG/01 REPORT of the Session of the Research Group of the Standing Technical Committee of the EUROPEAN COMMISSION FOR THE CONTROL OF FOOT-AND-MOUTH DISEASE held at Island of Moen, Denmark 12-15 September 2001 FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS Rome, 2001 ii TABLE OF CONTENTS Page INTRODUCTION .......................................................................................................... 1 Adoption of the Agenda ............................................................................................................. 3 Item 1 General information on the FMD situation in the world ..................................... 4 Item 2 Reports on outbreaks in Europe ........................................................................... 5 Item 3 Reports on field and laboratory experiences during the crisis in Europe ............ 7 Item 4 Special session on new kits by private companies and IAEA ................................ 9 Item 5 Serosurveillance ..................................................................................................... 11 Item 6 Subclinical infection and carrier stages ................................................................ 12 Item 7 FMD diagnostics .................................................................................................... 12 Item 8 Pathogenicity .......................................................................................................... 13 Item 9 Risk analysis and expert elicitation ........................................................................ 14 Item 10 Vaccines and antigen banks: new type O in Turkey; Review of the list ................. 14 of strains to be included in the banks Item 11 European Pharmacopoeia ..................................................................................... 15 Other items : Report of the workshop on the simulation exercise held in .................................. 16 Brno, 5-7 June 2001 Presentation of the new Reference Laboratory for Vesicular ............................... 16 Diseases (CERVES) at ISZLE, Brescia, Italy EC/EUFMD project .............................................................................................. 16 FMD in small camelid ........................................................................................... 16 Venues for the next Sessions of the Research Group ............................................ 16 Adoption of the Report .............................................................................................. 16 Closing remarks .......................................................................................................... 16 iii LIST OF APPENDICES Page Appendix 1 ................................................................................................................................. 17 FMD situation in Europe and over the world in 2001 John Ryan Appendix 2 ................................................................................................................................. 34 The situation of FMD in Turkey Nilay Ünal Appendix 3 .................................................................................................................................. 38 Molecular epidemiology of foot-and-mouth disease virus: The current situation in Europe and the Middle East N.J. Knowles, P.R. Davies and A.R. Samuel Appendix 4 .................................................................................................................................. 52 Sequence data of the foot-and-mouth disease outbreaks in the Netherlands; how do they correspond with the results from tracing A. Dekker, C. Boonstra-Leendertse, J. Boonstra Appendix 5 .................................................................................................................................. 54 Report on the outbreak in France François Moutou Appendix 6 .................................................................................................................................. 55 The epidemic of foot-and-mouth disease in the Netherlands in 2001: laboratory examinations A. Bouma, P. Eble, E. v. Rooij, A. Bianchi, A. Dekker Appendix 7 .................................................................................................................................. 58 Pirbright’s role in the UK 2001 FMD epidemic and its response to the emergency Soren Alexandersen, Paul Kitching and Alex I. Donaldson Appendix 8 .................................................................................................................................. 60 Report on the production of emergency O1 Manisa foot-and-mouth disease vaccine for the UK by the International Vaccine Bank (IVB), 22 March – 4 April 2001 Paul V. Barnett Appendix 9 .................................................................................................................................. 64 The Spanish diagnosis experience during the 2001 FMD European crisis Esther Blanco, Luis J. Romero, M.J. Zamora, M.J. Arias and José Sánchez-Vizcaíno Appendix 10 ............................................................................................................................... 65 Surveillance of FMD in Italy during 2001 A. Berlinzani, F. De Simone, M. Bugnetti, F. Fallacara and E. Brocchi iv Page Appendix 11 ................................................................................................................................ 69 Preventive measures and disease vigilance in Denmark following the current outbreak of FMD in the UK Per Have, Karin de Stricker and Karl Johan Sørensen Appendix 12 ................................................................................................................................ 71 Preventive measures and laboratory examinations in Germany following the current outbreaks of FMD in the EU Bernd Haas Appendix 13 ................................................................................................................................ 73 The FMD crisis 2001: field measures, laboratory tests and procedure for mass screening in Belgium Kris De Clercq, Karen Luyten, Koen Mintiens and Pierre Kerkhofs Appendix 14 ................................................................................................................................ 77 The 2001 FMD outbreak in the EU: an unforeseen interest for the disease in Switzerland Chris Griot Appendix 15 ................................................................................................................................ 78 Validation of the FAO Type O reference sera using sera collected on outbreak farms A. Dekker, F. van Hemert-Kluitenberg, K. Miedema, G. Chénard Appendix 16 ................................................................................................................................ 80 Intervet: Presentation of ELISA kit developed with Brescia and Pirbright Nico Visser Appendix 17 ................................................................................................................................ 84 Bayer: Results achieved so far with the test for antibodies against NSP’s of FMDV Joachim Grunmach Appendix 18 ................................................................................................................................ 88 Merial: Presentation of data Tim Doel Appendix 19 ................................................................................................................................ 94 Biomedical: Presentation of ELISA tests on NSP United Biomedical Inc. Appendix 20 ................................................................................................................................ 98 Experience of IAEA John Crowther Appendix 21 ................................................................................................................................ 103 The results of the serological surveillance following the vaccination in Thrace region in 2000 N. Bulut, U. Parlak, N. Ünal v Page Appendix 22 ............................................................................................................................... 112 The results of the 3ABC ELISA serosurvey conducted with the sera obtained from Thrace N. Bulut, U. Parlak, N. Ünal Appendix 23 ............................................................................................................................... 114 Report on the Workshop held in Lelystad on 28 and 29 June 2001 Aldo Dekker, Kris De Clercq Appendix 24 ............................................................................................................................... 117 Carrier sheep discovered during the Dutch foot-and-mouth disease Epidemic: A case report A. Dekker, J.M.A. Pol Appendix 25 ............................................................................................................................... 118 Evaluation of automated RT-PCR systems to accelerate FMD diagnosis Scott M. Reid, Nigel P. Ferris, Geoffrey H. Hutchings and Soren Alexandersen Appendix 26 ................................................................................................................................ 126 A novel method for detection of FMDV from culture and clinical samples by RT-PCR and Restriction Enzyme Analysis Margarita Sáiz, Diana B. de la Morena,