Supplementary Information Table 1 Probe M B Description NM_004253 1.12 10.49 PHOSPHOLIPASE A2-ACTIVATING PROTEIN (PLAA) NM_005084 1.40 8.01 PHOSPHOLIPASE A2, GROUP VII (PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE, PLASMA) (PLA2G7) NM_005017 0.89 7.71 PHOSPHATE CYTIDYLYLTRANSFERASE 1, CHOLINE, ALPHA ISOFORM (PCYT1A) NM_020244 1.41 7.09 CHOLINEPHOSPHOTRANSFERASE 1 (CHPT1) NM_001277 0.79 5.78 CHOLINE KINASE (CHK) NM_002660 0.80 3.49 PHOSPHOLIPASE C, GAMMA 1 (FORMERLY SUBTYPE 148) (PLCG1) NM_005090 -0.70 3.34 PHOSPHOLIPASE A2, GROUP IVB (CYTOSOLIC) (PLA2G4B) AJ272267 0.78 1.15 HOMO SAPIENS PARTIAL FOR CHOLINE DEHYDROGENASE (CHDH GENE) NM_003706 0.33 0.19 PHOSPHOLIPASE A2, GROUP IVC (CYTOSOLIC, CALCIUM-INDEPENDENT) (PLA2G4C) 949518CB1 -0.30 -0.49 PHOSPHOLIPASE A2, GROUP IB (PANCREAS) (PLA2G1B), NUCLEAR GENE ENCODING MITOCHONDRIAL PROTEIN NM_003574 -0.44 -0.82 VAMP (VESICLE-ASSOCIATED MEMBRANE PROTEIN)-ASSOCIATED PROTEIN A (33KD) (VAPA) NM_006330 0.76 -2.28 LYSOPHOSPHOLIPASE I (LYPLA1) NM_002662 0.66 -2.68 PHOSPHOLIPASE D1, PHOPHATIDYLCHOLINE-SPECIFIC (PLD1) NM_004573 -0.28 -2.95 PHOSPHOLIPASE C, BETA 2 (PLCB2) NM_003560 0.31 -3.40 PHOSPHOLIPASE A2, GROUP VI (CYTOSOLIC, CALCIUM-INDEPENDENT) (PLA2G6) NM_002661 0.22 -4.40 PHOSPHOLIPASE C, GAMMA 2 (PHOSPHATIDYLINOSITOL-SPECIFIC) (PLCG2) NM_000300 0.60 -4.43 PHOSPHOLIPASE A2, GROUP IIA (PLATELETS, SYNOVIAL FLUID) (PLA2G2A), NUCLEAR GENE ENCODING MITOCHONDRIAL PROTEIN NM_002663 0.30 -4.45 PHOSPHOLIPASE D2 (PLD2) NM_000933 0.37 -4.45 PHOSPHOLIPASE C, BETA 4 (PLCB4) 159053.3 -0.20 -5.76 PHOSPHOLIPASE A2, GROUP IB (PANCREAS) (PLA2G1B), NUCLEAR GENE ENCODING MITOCHONDRIAL PROTEIN 2899129CB1 -0.15 -6.32 LYSOPHOSPHOLIPASE 3 (LYSOSOMAL PHOSPHOLIPASE A2) (LYPLA3) NM_006226 0.28 -6.37 PHOSPHOLIPASE C, EPSILON (PLCE) NM_000929 -0.33 -6.72 PHOSPHOLIPASE A2, GROUP V (PLA2G5) NM_015715 -0.14 -6.78 GROUP III SECRETED PHOSPHOLIPASE A2 (GIII-SPLA2) 232063 -0.09 -6.88 CDNA FLJ36326 FIS, CLONE THYMU2005576, MODERATELY SIMILAR TO MUS MUSCULUS GROUP IIF SECRETED PHOSPHOLIPASE A2 (PLA2G2F) AF038440 0.47 -6.92 PHOSPHOLIPASE D2 (PLD2) , SPLICE VARIANT HPLD2A, COMPLETE CDS AF263613 -0.48 -7.16 MEMBRANE-ASSOCIATED CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 GAMMA , COMPLETE CDS NM_021213 0.06 -7.36 PHOSPHATIDYLCHOLINE TRANSFER PROTEIN (PCTP) NM_007366 0.07 -7.38 PHOSPHOLIPASE A2 RECEPTOR 1, 180KD (PLA2R1) NM_032562 -0.10 -7.39 PHOSPHOLIPASE A2, GROUP XIII (PLA2G13) NM_003561 0.08 -7.40 PHOSPHOLIPASE A2, GROUP X (PLA2G10) NM_012400 -0.07 -7.46 PHOSPHOLIPASE A2, GROUP IID (PLA2G2D) AB005216 -0.09 -7.53 FOR NCK, ASH AND PHOSPHOLIPASE C GAMMA-BINDING PROTEIN NAP4, PARTIAL CDS NM_000932 -0.07 -7.55 PHOSPHOLIPASE C, BETA 3 (PHOSPHATIDYLINOSITOL-SPECIFIC) (PLCB3) NM_007260 -0.03 -7.66 LYSOPHOSPHOLIPASE II (LYPLA2) NM_006090 -0.05 -7.69 CHOLINE/ETHANOLAMINEPHOSPHOTRANSFERASE (CEPT1) NM_004845 0.04 -7.71 PHOSPHATE CYTIDYLYLTRANSFERASE 1, CHOLINE, BETA ISOFORM (PCYT1B) NM_020549 -0.02 -7.74 CHOLINE ACETYLTRANSFERASE (CHAT), TRANSCRIPT VARIANT M 1503336.16 0.03 -7.75 PHOSPHOLIPASE A2, GROUP IB (PANCREAS) (PLA2G1B), NUCLEAR GENE ENCODING MITOCHONDRIAL PROTEIN NM_005198 -0.01 -7.75 CHOLINE KINASE-LIKE (CHKL) NM_006225 0.00 -7.75 PHOSPHOLIPASE C, DELTA 1 (PLCD1) Table 1 Differential expression of genes involved in choline metabolism between HCT116 wild-type and p300- cells. The values, M and B, were calculated for each element on the array. M is a measure of differential gene expression - and is based on log2 of the ratio p300 /HCT116. B is a statistic for likelihood of difference used to rank genes. Highlighted genes represent genes involved in choline metabolism where expression levels appear to be significantly different between p300- and HCT116 wild-type cells. .