Wednesday, 2 October 2019 One vaccine, two diseases, three lessons Helen Petousis-Harris, PhD Senior Lecturer, Vaccinology Dept General Practice and Primary Health Care Overview Virtues of an outer Kissing cousins, two Serendipity and membrane vesicle vaccine? divergent diseases opportunity Wednesday, October 2, 2019 • Most meningococcal vaccines based on polysaccharide antigens (groups A, C, W135, Y) • Meningococcal Group B oligosaccharides cross react with fetal neuro tissue – not suitable vaccine antigen • Meningococcal Group B vaccine approaches needed to be 3 different – non PS-Conjugate Solution – outer membrane vesicles There are many other antigenic structures aside from PS Outer-membrane vesicles generate mainly strain specific responses against PorA which is highly variable across strains Developed in 1980’s and used Cuba and Norway 4 2/10/2019 Tan LKK, Carlone GM, Borrow R. Advances in the Development of Vaccines against Neisseria meningitidis. New England Journal of Medicine. 2010;362:1511-20. Meet the family 80-90% homology in primary sequences High level of recombination 5 Muzzi, A., Mora, M., Pizza, M., Rappuoli, R., & Donati, C. (2013). Conservation of meningococcal antigens in the genus Neisseria. MBio, 4(3), e00163-13. What is gonorrhoea? 6 2/10/2019 How common is gonorrhoea? • Second most reported sexually transmitted disease in US (600,000 cases per annum) • In NZ ~3000 cases per annum (60-90 per 100,000) • To put in context – Invasive Pneumococal Disease pre-vaccine <2s ~100 per 100,000 – Meningococcal disease at its height 17 per 100,000 • Tairawhiti DHB 400 per 100,000 7 No correlate of protection and repeat infections • Natural infection with gonorrhoea does not induce a protective immune response. – Repeated infection • Spontaneous resolution – Weeks to months • Over 100 years of effort but no vaccine • Lucky penicillin came along… Biographer James Boswell by George Willison. Wiki 8 2/10/2019 Gonorrhoea resistance to antimicrobials is growing rapidly X Sulfanilamide 1940s X Penicillins 1980s X Tetracyclines 1980s X Fluoroquinolones 2007 X Extended spectrum cephalosporins (increasing) • Multidrug resistant and extensively drug resistant The recent emergence of the XDR gonococcal ‘superbug’ strongly argues the case for investment in research and development for both novel antimicrobial agents and, most importantly of all, a gonococcal vaccine. Lewis DA. Global resistance of Neisseria gonorrhoeae: when theory becomes reality. Current opinion in infectious diseases. 2014;27(1):62-7. Bolan GA, Sparling PF, Wasserheit JN. The emerging threat of untreatable gonococcal infection. N Engl J Med. 2012;366(6):485-7. 9 2/10/2019 Short list of epic fails • Only 4 candidates advanced to clinical trials – Whole cell early 1900s – Partially autolyzed 1974 – Pilus-based 1990s – Protein 1-based vaccine • None worked Edwards JL, Jennings MP, Apicella MA, Seib KL. Is gonococcal disease preventable? The importance of understanding immunity and pathogenesis in vaccine development. Critical Reviews in Microbiology. 2016:1-14. 10 Challenges • Appropriate infection models • Variability of antigens • Blocking of Ab to conserved antigens • No correlate of protection, little natural immunity Vaccine development will likely require the production of a immune response that is different to natural infection Edwards JL, Jennings MP, Apicella MA, Seib KL. Is gonococcal disease preventable? The importance of understanding immunity and pathogenesis in vaccine development. Critical Reviews in Microbiology. 2016:1-14. 11 2/10/2019 In contrast Holst J, Oster P, Arnold R, Tatley MV, Naess LM, Aaberge IS, et al. Vaccines against meningococcal serogroup B disease containing outer membrane vesicles (OMV): lessons from past programs and implications for the future. Human vaccines & immunotherapeutics. 2013;9(6):1241-53. 12 2/10/2019 July 7 2017 – WHO call to action, situation has become desperate 7 July 2017 7 July 2017 13 2/10/2019 87 million New gonorrhoea cases per year Rowley J, Hoorn SV, Korenromp E, et al. Chlamydia, gonorrhoea, trichomoniasis and syphilis: global prevalence and incidence estimates, 2016. Bulletin of the World Health Organization, 2019. 14 2/10/2019 Lesson one Gonorrhoea is a disease in desperate want of a vaccine 15 2/10/2019 NZ had an epidemic necessitating a tailor made Nm vaccine MeNZB™ Problem Clone expressing the P1.7-2.4 PorA protein OMV Vaccine based on Solution Norwegian MenBvac (MeNZB™) Group A Group B Partnership between the NIPH, Strategy (then) Chiron*, the University of Auckland, the Institute of Environmental Science and Research (ESR) and the WHO *predecessor to Novartis Vaccines/Novartis Vaccines & Diagnostics AG Arnold R, Galloway Y, McNicholas A, O'Hallahan J. Effectiveness of a vaccination programme for an epidemic of meningococcal B in New Zealand. Vaccine. 2011;29(40):7100-6. 16 Lopez L, Sherwood J. The Epidemiology of meningococcal disease in New Zealand 2013. Wellington, New Zealand.: Institute of Environmental Science and Research Ltd (ESR),; 2014. 2/10/2019 New Zealand MeNZB™ 3+0 delivered 6 weeks apart 17 2/10/2019 The MeNZB™ Programme sought to vaccinate NZers under 20 years 2002-2004 Phase I and II trials Mass campaign to immunise 2004-2006 population under 20 years in staggered roll out 3+0 0, 6, 12 weeks One million individuals vaccinated 2004- 2006 Coverage in children up to 15y ~80%, 15 – 17 y ~50% Infant National Immunisation Programme 2006-2008 Rayvin Ah Him, first infant to receive MeNZB, with NZ Prime Minister Helen Clark. NZ Herald Withdrawn 2008 no co-administration data PCV v. low uptake infants 18 2/10/2019 Impact of MeNZB™ was moderate The epidemic had waned by the time the vaccine was developed and rolled out. Holst J, Oster P, Arnold R, Tatley MV, Naess LM, Aaberge IS, et al. Vaccines against meningococcal serogroup B disease containing outer membrane vesicles (OMV): lessons from past programs and implications for the future. Human vaccines & immunotherapeutics. 2013;9(6):1241-53. 19 Vaccine Effectiveness (VE) of MeNZB™ was moderate • 73% (52–85%)1 VE against strain- • 77% (62–85%)2 after mean 3.5 years specific disease • 68%* VE against non- • 56% (17–77%) epi group B VE against all • 67% (57–76%) against all meningococcal meningococcal disease *VE against invasive pneumococcal disease to measure residual confounding 56% (44– 66%). Reduced the VE for group B to 68% 1.Kelly C, Arnold R, Galloway Y, O'Hallahan J. A prospective study of the effectiveness of the New Zealand meningococcal B vaccine. American journal of epidemiology 2007;166:817-23. 2.Arnold R, Galloway Y, McNicholas A, O'Hallahan J. Effectiveness of a vaccination programme for an epidemic of meningococcal B in New Zealand. Vaccine 2011;29:7100-6. 20 2/10/2019 What does this have to do with the clap? 21 2/10/2019 Reported gonorrhoea in NZ Gonorrhoea rates in selected regions, 1998–2016. The Institute of Environmental Science and Research Ltd. Sexually transmitted infections in New Zealand. Annual Surveillance Report 2013. Porirua, New Zealand: The Institute of Environmental Science and Research Ltd; 2016. 22 2/10/2019 Reported chlamydia in NZ Chlamydia rates in selected regions 1998 – 2016. The Institute of Environmental Science and Research Ltd. Sexually transmitted infections in New Zealand. Annual Surveillance Report 2013. Porirua, New 23 Zealand: The Institute of Environmental Science and Research Ltd; 2016. 2/10/2019 Morbidity of Neisseria pathogenic species since 1970 in Cuba. Nm sustained to 2016 0.1/100,000 Pérez O, J dC, M C, et al. Mucosal approaches in Neisseria vaccinology. VacciMonitor 2009;18:53-5. 24 2/10/2019 Ministerio De Salud Publica (2017). "Annula Estadistico De Salud 2016". (La Habana). Key point Several lines of indirect evidence suggest some degree of protection against gonorrhoea is possible following the administration of group B meningococcal vaccines. 25 2/10/2019 Lesson two Carpe diem 26 2/10/2019 We did a case-control study using data- linkage All adolescents and adults aged 15–30 years (born between 1984 and 1998) attending Sexual Health Clinics who were diagnosed with gonorrhoea, chlamydia or both, and eligible to receive the MeNZB™ vaccine in NZ from 2004–2008. 27 2/10/2019 Cases and controls had similar risk factors including routine testing for both Lab Lab confirmed confirmed chlamydia gonorrhoea 1241 1002 12487 Cases Co-infected Controls 28 2/10/2019 VE and duration of protection 31% (95% CI 21–39%) * * * The difference in the bars represent vaccine effectiveness. *= years where significant difference Petousis-Harris H, Paynter J, Morgan J, Saxton P, McArdle B, Goodyear-Smith F, et al. Effectiveness of a group B outer membrane vesicle 29 meningococcal vaccine against gonorrhoea in New Zealand: a retrospective case-control study. Lancet. 2017;390:1603-10. 2/10/2019 Editorial* Podcast Altmetric 1205 #94/31,523 Lancet outputs (0.003%) Study 1 .0002% all 13million research outputs tracked 30 *Seib KL. Gonorrhoea vaccines: a step in the right direction. The Lancet. 2017. 2/10/2019 The cohort study Access to the data presented was managed by Statistics New Zealand under strict micro-data access protocols and in accordance with the security and confidentiality provisions of the Statistic Act 1975. Our findings are not Official Statistics. The opinions, findings, recommendations, and conclusions expressed are those of the authors, not Statistics NZ. 31 2/10/2019 ❖Population NZrs eligible for the MeNZB vaccine who were born 1984-1999 ❖Excluded included immigrated, inactive, died ❖Cases were hospitalized with a gonorrhea diagnosis only ❖Analysis included sensitivity with less specific outcome codes ❖Cox’s prop hazards models with Firth correction for rare outcomes ❖VE 1-HR The MeNZB vaccine was effective at preventing gonorrhoea related hospitalisations N=935,496 VE 24% (95% CI 1-42%) VE 47% (95% CI 18-66%) Paynter, J., Goodyear-Smith, F., Morgan, J., Saxton, P., Black, S., & Petousis-Harris, H. (2019). Effectiveness of a group B outer membrane vesicle meningococcal vaccine in preventing hospitalization from gonorrhea in New Zealand: a retrospective cohort study.