SAN TA C RUZ BI OTEC HNOL OG Y, INC . Lipotropin γ (E-19): sc-33036 BACKGROUND APPLICATIONS Pro-opiomelanocortin (POMC) is a precursor hormone of the pituitary gland Lipotropin γ (E-19) is recommended for detection of POMC and the that undergoes enzymatic cleavage to yield active ligands for melanocortin processed active peptide Lipotropin γ of mouse, rat and human origin receptors. These peptides include adrenocorticotropin (ACTH), corticotropin- by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), like intermediate lobe peptide (CLIP), melanotropins ( α, β, γ-MSH), lipotropin immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell β (β-LPH), Lipotropin γ (γ-LPH) and endorphins. ACTH stimulates the adrenal lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- glands to release cortisol and is produced by the pituitary gland. MSH (mela- 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30- nocyte-stimulating hormone) is also produced by the pituitary gland and 1:3000). functions by increasing skin pigmentation by increasing melanocyte melanin Suitable for use as control antibody for POMC siRNA (h): sc-37277, POMC production. Defects in the POMC gene may cause a genetic predisposition to siRNA (m): sc-37278, POMC shRNA Plasmid (h): sc-37277-SH, POMC shRNA obesity. Lipotropin , also known as -LPH, is a 56 amino acid active peptide γ γ Plasmid (m): sc-37278-SH, POMC shRNA (h) Lentiviral Particles: sc-37277-V which is the N-terminal peptide fragment of Lipotropin . Lipotropin stim - β β and POMC shRNA (m) Lentiviral Particles: sc-37278-V. ulates melanocytes to produce melanin. Molecular Weight of POMC precursor: 30 kDa. REFERENCES Molecular Weight of Lipotropin γ: 5 kDa. 1. Millington, G.W., et al. 2001. Differential effects of -, - and (2)- α β γ Positive Controls: POMC (h): 293T Lysate: sc-111490. melanocyte-stimulating hormones on hypothalamic neuronal activation and feeding in the fasted rat. Neuroscience 108: 437-445. RECOMMENDED SECONDARY REAGENTS 2. Grässel, S., et al. 2009. The melanocortin system in articular chondro - To ensure optimal results, the following support (secondary) reagents are cytes: melanocortin receptors, pro-opiomelanocortin, precursor proteases, recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 and a regulatory effect of α-melanocyte-stimulating hormone on proin - (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey flammatory cytokines and extracellular matrix components. Arthritis anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ Rheum. 60: 3017-3027. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 3. McLaughlin, P.J., et al. 2009. Growth inhibition of thyroid follicular cell- sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- derived cancers by the opioid growth factor (OGF)-opioid growth factor itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). receptor (OGFr) axis. BMC Cancer 9: 369. 3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 4. Belgardt, B.F., et al. 2009. Hormone and glucose signalling in POMC and 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. AgRP neurons. J. Physiol. 587: 5305-5314. DATA CHROMOSOMAL LOCATION Genetic locus: POMC (human) mapping to 2p23.3; Pomc (mouse) mapping to AB 12 A1.1. 55K– 43K– SOURCE < 34K– POMC Lipotropin γ (E-19) is an affinity purified goat polyclonal antibody raised against a peptide mapping near the C-terminus of POMC of human origin. 23K– PRODUCT Lipotropin γ (E-19): sc-33036. Western blot analysis of Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide POMC expression in non-transfected: sc-117750 (A) and 0.1% gelatin. and human POMC transfected: sc-111490 (B) whole cell lysates. Blocking peptide available for competition studies, sc-33036 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and STORAGE support products. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. RESEARCH USE For research use only, not for use in diagnostic procedures. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.