ii Contents University of Windsor Health and Safety Policy Statement ................................................................... 1 Laboratory Safety .................................................................................................................................................. 2 Introduction .................................................................................................................................................. 2 Responsibilities ............................................................................................................................................. 2 Supervisors .................................................................................................................................................... 2 Laboratory Personnel .................................................................................................................................... 3 Laboratory Instructors .................................................................................................................................. 4 Visitors .......................................................................................................................................................... 4 Health and Safety .......................................................................................................................................... 4 Chemical Control Centre ............................................................................................................................... 5 General Laboratory Safety Principals ............................................................................................................ 6 General Guidelines ........................................................................................................................................ 6 Preparing for Laboratory Work ..................................................................................................................... 7 Evaluating Laboratory Hazards… an Ongoing Process .................................................................................. 7 During Laboratory Work ............................................................................................................................... 8 Unattended Procedures/Experiments .......................................................................................................... 8 Prior to leaving .............................................................................................................................................. 9 Working Alone Policy .................................................................................................................................... 9 Workplace Hazardous Materials Information System (WHMIS) ...................................................... 10 Labeling ....................................................................................................................................................... 11 Material Safety Data Sheet (MSDS) ............................................................................................................ 13 Hazard Warning Symbols ............................................................................................................................ 16 Flammable chemicals .................................................................................................................................. 16 Oxidizing Chemicals .................................................................................................................................... 17 Toxic Chemicals ........................................................................................................................................... 18 Corrosive Chemicals .................................................................................................................................... 19 Hazardous Materials Properties ................................................................................................................. 20 Personal Protective Equipment (PPE) ........................................................................................................ 22 Gloves .......................................................................................................................................................... 24 Personal Protective Equipment Symbols .................................................................................................... 25 Compressed Gas Cylinders .............................................................................................................................. 26 Introduction ................................................................................................................................................ 26 Storage ........................................................................................................................................................ 27 Handling ...................................................................................................................................................... 27 Transportation ............................................................................................................................................ 29 Leaks ........................................................................................................................................................... 29 Cryogenic Liquids and Dry Ice ....................................................................................................................... 30 Cryogenic Liquid Containers ....................................................................................................................... 30 Hazards Associated with Cryogens ............................................................................................................. 31 Control of Hazards ...................................................................................................................................... 32 Special Handling Procedures and Storage Requirements ........................................................................... 32 Solid Carbon Dioxide (dry ice) ..................................................................................................................... 32 Transportation of Cryogens on Campus ..................................................................................................... 32 First Aid ....................................................................................................................................................... 33 iii Asphyxiation ................................................................................................................................................ 34 Laboratory Ventilation and Chemical Fume Hoods ............................................................................... 35 General Ventilation ..................................................................................................................................... 35 Local Ventilation Devices ............................................................................................................................ 35 Chemical Fume Hoods ................................................................................................................................ 35 Elephant Trunks .......................................................................................................................................... 37 Canopy Fume Hood ..................................................................................................................................... 38 Slotted Hoods .............................................................................................................................................. 38 Biological Safety Cabinets (BSC) .................................................................................................................. 38 Glove Box .................................................................................................................................................... 41 Biological Hazard ................................................................................................................................................ 42 Radiation Hazard ................................................................................................................................................ 42 Chemical Hazards ............................................................................................................................................... 43 Chemical Storage ................................................................................................................................................ 45 General Rules of Safe Chemical Storage – Code of Practice ....................................................................... 45 Chemical Compatibility and Simplified Segregation Scheme ..................................................................... 46 Explosive chemicals ..................................................................................................................................... 48 Materials Liable to Form Peroxides in Storage ........................................................................................... 49 Explosive Boiling.........................................................................................................................................