For Research Use Only P2RX4 Polyclonal antibody Catalog Number:13534-1-AP Featured Product 4 Publications www.ptglab.com Catalog Number: GenBank Accession Number: Purification Method: Basic Information 13534-1-AP BC033826 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 500 μg/ml by 5025 WB 1:500-1:1000 Nanodrop and 200 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:1000 method using BSA as the standard; purinergic receptor P2X, ligand-gated for WB Source: ion channel, 4 IHC 1:20-1:200 IF 1:20-1:200 Rabbit Calculated MW: Isotype: 388 aa, 43 kDa IgG Observed MW: Immunogen Catalog Number: 70-80 kDa, 50-60 kDa AG4445 Applications Tested Applications: Positive Controls: FC, IF, IHC, IP, WB, ELISA WB : A549 cells, HeLa cells, SH-SY5Y cells Cited Applications: IP : mouse brain tissue, IF, IHC IHC : human kidney tissue, human lung tissue, human Species Specificity: ovary tissue, human placenta tissue, human skin human, mouse tissue, human spleen tissue Cited Species: human, mouse, rat IF : HeLa cells, Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 Background Information receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439). Notable Publications Author Pubmed ID Journal Application Zhuo Lin 33828988 Front Oncol IHC Ting Long 31937253 J Headache Pain IHC Yuyin Zheng 33398365 Mol Med Rep IF Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data WB result of P2RX4 antibody (13534-1-AP; 1:1000; A549 cells were subjected to SDS PAGE followed by Immunofluorescent analysis of HeLa cells using incubated at room temperature for 1.5 hours) with western blot with 13534-1-AP (P2RX4 antibody) at 13534-1-AP (P2RX4 antibody) at dilution of 1:50 sh-Control and sh-P2RX4 transfected A549 cells. dilution of 1:800 incubated at room temperature for and Alexa Fluor 488-conjugated AffiniPure Goat 1.5 hours. Anti-Rabbit IgG(H+L). 1X10^6 HeLa cells were stained with 0.2ug P2RX4 Immunohistochemical analysis of paraffin- Immunohistochemical analysis of paraffin- antibody (13534-1-AP, red) and control antibody embedded human kidney using 13534-1-AP (P2RX4 embedded human kidney using 13534-1-AP (P2RX4 (blue). Fixed with 90% MeOH blocked with 3% BSA antibody) at dilution of 1:100 (under 10x lens). antibody) at dilution of 1:100 (under 40x lens). (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. IP Result of anti-P2RX4 (IP:13534-1-AP, 4ug; Detection:13534-1-AP 1:500) with mouse brain tissue lysate 4000ug..