EEXXEERRCCIISSEE IIMMMMUUNNOOLLOOGGYY RREEVVIIEEWW VOLUME 21 • 2015 The International Society of Exercise and Immunology EXERCISEEXERCISE IMMUNOLOGY IMMUNOLOGY REVIEW REVIEW An official Publication of ISEI and DGSP We gratefully acknowledge and thank for years of sponsoring by the Verein zurK Förderungarsten K derrüg Sportmediziner for the editorial team and all our readers Hinnak Northoff for the editorial team and all our readers EXERCISE VOLUME 21 • 2015 IMMUN OLOGY REVIEW CONTENTS From the Editors 7 Reviews Cytokine expression and secretion by skeletal muscle cells: regulatory mechanisms and exercise effects Jonathan M. Peake, Paul Della Gatta, Katsuhiko Suzuki, David C. Nieman 8 Exercise and inflammation-related epigenetic modifications: focus on DNA methylation. Steven Horsburgh, Paula Robson-Ansley, Rozanne Adams, Carine Smith 26 Exercise, skeletal muscle and inflammation: ARE-binding proteins as key regulators in inflammatory and adaptive networks Thomas Beiter, Miriam Hoene, Frauke Prenzler, Frank C. Mooren, Jürgen M. Steinacker, Cora Weigert, Andreas M. Nieß, Barbara Munz 42 Metabolic signals and innate immune activation in obesity and exercise Robert Ringseis, Klaus Eder, Frank C. Mooren, Karsten Krüger 58 The microbiota: An exercise immunology perspective Stéphane Bermon, Bernardo Petriz, Alma Kajėnienė, Jonato Prestes, Lindy Castell, Octavio L. Franco 70 Understanding graft-versus-host disease. Preliminary findings regarding the effects of exercise in affected patients Carmen Fiuza-Luces, Nuria Garatachea, Richard J. Simpson, Nathan A. Berger, Manuel Ramírez, Alejandro Lucia 80 ORiginal ReseaRch Circulatory endotoxin concentration and cytokine profile in response to exertional-heat stress during a multi-stage ultra-marathon competition Samantha K Gill, Ana Teixeira, Luis Rama, Fatima Rosado, Joanne Hankey, Volker Scheer, Krystal Hemmings, Paula Ansley-Robson, Ricardo JS Costa 114 Changes of thioredoxin, oxidative stress markers, inflammation and muscle/renal damage following endurance exercise Kaoru Sugama, Katsuhiko Suzuki, Kayo Yoshitani, Koso Shiraishi, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori, Takashi Kometani 130 EIR 21 2015 CONTENTS A single bout of dynamic exercise enhances the expansion of MAGE-A4 and PRAME-specific cytotoxic T-cells from healthy adults Emily C.P. LaVoy, Catherine M. Bollard, Patrick J. Hanley, James W. Blaney, Daniel P. O’Connor, Jos A. Bosch, Richard J. Simpson 144 Influence of age and physical fitness on miRNA-21, TGF- β and its receptors in leukocytes of healthy women Barbara Halper, Marlene Hofmann, Stefan Oesen, Bernhard Franzke, Petra Stuparits, Claudia Vidotto, Harald Tschan, Norbert Bachl, Eva-Maria Strasser, Michael Quittan, Karl-Heinz Wagner, Barbara Wessner 154 Exercise-induced increases in cell free DNA in human plasma originate predominantly from cells of the haematopoietic lineage Suzan Tug, Susanne Helmig, Eva Ricarda Deichmann, Anna Schmeier-Jürchott, Eva Wagner, Tim Zimmermann, Markus Radsak, Mauro Giacca, Perikles Simon 164 Inflammatory cytokine kinetics to single bouts of acute moderate and intense aerobic exercise in women with active and inactive systemic lupus erythematosus Luiz Augusto Perandini, Diego Sales-de-Oliveira, Suzana Beatriz Verissimo Mello, Niels Olsen Camara, Fabiana Braga Benatti, Fernanda Rodrigues Lima, Eduardo Borba, Eloisa Bonfa, Hamilton Roschel, Ana Lúcia de Sá-Pinto, Bruno Gualano 174 letteRs The evidence of exercise-induced bronchoconstriction in endurance runners; genetic basis and gender differences Asghar Abbasi, Rodolfo de Paula Vieira, Hinnak Northoff 186 Instructions for authors of EIR 189 EIR 21 2015 Exercise Immunology Review Editorial Statement Exercise Immunology Review, an official publication of the International Exercise Immunology Review publishes review articles that explore: Society of Exercise Immunology and of the German Society of Sports Medi - (a) fundamental aspects of immune function and regulation during exercise; cine and Prevention, is committed to developing and. enriching knowledge in (b) interactions of exercise and immunology in the optimization of health and all aspects of immunology that relate to sport, exercise, and regular physical protection against acute infections: (c) deterioration of immune function ativity. In recognition of the broad range of disciplines that contribute to the resulting from competitive stress and overtraining; (d) prevention or modula - understanding of immune function, the journal has adopted an interdiscipli - tion of the effects of aging or disease (including HIV infection; cancer; nary focus. This allows dissemination of research findings from such disci - autoimmune, metabolic or transplantation associated disorders) through exer - plines as exercise science, med ici ne, immunology, physiology, behavioral sci - cise. (e) instrumental use of exercise or related stress models for basic or ence, endocrinology, pharmacology, and psychology . applied research in any field of physiology, pathophysiology or medicine with relations to immune function. € Exercise Immunology Review (ISSN 1077-5552) is published and sponsored Subscription rates are $45 in the US and 40 in Europe and other countries. annually by the Association for the Advancement of Sports Medicine (Verein zur Postmaster: Förderung der Sportmedizin) and printed by TOM-Systemdruck GmbH, Hansan - Justus-Liebig-Universität Gießen, Abteilung für Sportmedizin ring 125. Dr. Karsten Krüger, Kugelberg 62, 35394 Gießen . Editor: Dr. Karsten Krüger Send editorial correspondence to: Past Editor: Prof. Dr. Hinnak Northoff Justus-Liebig- Universität Gießen Abteilung für Sportmedizin Dr. Karsten Krüger Kugelberg 62 35394 Gießen [email protected] Copyright © 2002 by Hinnak Northoff. Exercise Immunology Review is indexed in Sport Database, Sport Discus, Sport Search, SciSearch, EMBASE / Excerpta Medica, Focus on: Sports Science & Medi cine, Index Medicus, MEDLINE , Physical Educa tion Index, Research Alert, International Bibliogra phy of Periodical Literature, International Bibliography of Book Reviews, and CINAHL database. Notice: authorization to photocopy items is granted for internal or personal use only. All other cases contact Hinnak Northoff. To subscribe or renew subscription go to http://www.isei.dk/ or e-mail: [email protected] EIR 21 2015 Editorial • 7 From the Editors EIR21 is a larger issue than usual containing 6 reviews, 6 cells from the haematopoietic lineage are the main source of original research articles and one letter. This is the result of an cfDNA released during acute bouts of exercise. Perandini et increase in the number of interesting manuscripts offered to al. analyzed the inflammatory cytokine kinetics to single us. The high number of submissions last year made it difficult bouts of exercise in women with active and inactive systemic to focus on special topics. Instead, it is a multifaceted compi - lupus erythematosus. Finally, the letter of Abbasi et al. pres - lation of articles on topics that are sufficiently novel and inter - ents some evidence of exercise-induced bronchoconstriction esting for our readership. in endurance runners and discusses some genetic aspects and The review of Peake et al. addresses the capacity of skele - gender differences. tal muscle cells to produce cytokines and examines potential other sources of circulating cytokines during exercise. The This year’s issue of EIR is the first one to appear under the review of Horsburgh et al. is about the actual topic of epige - new Editor Team with me (Karsten Krüger), closely supported netics and focusses on DNA methylation in the context of by Mike Gleeson and Jonathan Peake. For EIR22 and the inflammation and exercise. The article of Munz et al. discuss - future we want the majority of contributions to be topical es the role of inflammation in skeletal muscle adaptation to review articles. In the case of original research articles we exercise with a focus on ARE-binding proteins. Ringseis et al. encourage the authors to embed their new data into review present an article about metabolic signals and innate immune articles. Please note that the submission deadline is 31st July activation for a better understanding of immunologic signal - 2015 for EIR22 which we aim to publish in early January ing in obesity prevention or therapy by exercise. The review 2016. We hope you enjoy reading the new issue and appreci - of Bermon et al. intends to shed some light upon the interac - ate the new format. In the future EIR will be only published tion between the gut microbiota, exercise and immunomodu - online. Only for special demands (like academic libraries) lation. The last review article of EIR21 is a comprehensive will there be printed versions available. article about effects of exercise on graft-versus-host disease. Personally, I thank you (all ISEI members), and all mem - The original research section starts with an article by Gill bers of the Editorial Board for the confidence you have placed et al. reporting that a multi-stage ultra-marathon competition in us. We hope to see you at the 12th Symposium of the Inter - in the heat resulted in a modest circulatory endotoxaemia national Society of Exercise and Immunology at 6-9 July accompanied by a pronounced pro-inflammatory cytoki - 2015 in Vienna. naemia and compensatory anti-inflammatory responses. The research article of Sugama et al. deals with changes in circu - Thank you, Mike Gleeson and Jonathan Peake, for the close lating thioredoxin, oxidative