Product datasheet [email protected] ARG23469 Package: 100 μg anti-CD300a + CD300c antibody [P192] Store at: -20°C Summary Product Description Mouse Monoclonal antibody [P192] recognizes CD300a + CD300c Mouse anti Human CD300a antibody, clone P192 specifically recognizes human CD300a, otherwise known as IRp60 (inhibitory receptor protein 60), a 60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.Studies have shown that the cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE- induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro. Tested Reactivity Hu Tested Application FACS Host Mouse Clonality Monoclonal Clone P192 Isotype IgG2a Target Name CD300a + CD300c Antigen Species Human Immunogen Polyclonal population of NK cells Conjugation Un-conjugated Alternate Names CMRF-35-H9; Inhibitory receptor protein 60; NK inhibitory receptor; CMRF35-H; CMRF35H9; CD antigen CD300a; IRC1/IRC2; IGSF12; CMRF35H; IRp60; IgSF12; CMRF35-like molecule 8; CMRF35-H9; CMRF-35H; CD300 antigen-like family member A; CLM-8; IRC1; IRC2; Immunoglobulin superfamily member 12 Application Instructions Application table Application Dilution FACS 1:25 - 1:200 Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 33 kDa Properties Form Liquid Purification Purification with Protein G. www.arigobio.com 1/2 Buffer PBS and 0.09% Sodium azide. Preservative 0.09% Sodium azide Concentration 1 mg/ml Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol CD300A Gene Full Name CD300a molecule Background This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] Function Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. [UniProt] Images ARG23469 anti-CD300a + CD300c antibody [P192] FACS image Flow Cytometry: Human peripheral blood monocytes stained with ARG23469 anti-CD300a + CD300c antibody [P192] followed by Rabbit F(ab')2 anti Mouse IgG (FITC). www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).