Calcium Detection Probes & AssayKits Fluo-8® Cal-520® Calbryte™520 AAT Bioquest® Our Mission AATBioquest®iscommitted to constantly meet or exceedits customer’srequirements by providing consistently high quality products and services,andby encouraging continuous improvements in its long-term and daily operations. Our core value is Innovation and Customer Satisfaction. Our Story AATBioquest®,Inc.(formerly ABDBioquest, Inc.)develops, manufactures and markets bioanalytical researchreagents and kits to life sciencesresearch,diagnostic R&Dand drug discovery. Wespecializein photometric detections including absorption (color), fluorescence and luminescence technologies. TheCompany'ssuperior products enable life scienceresearchersto better under- stand biochemistry, immunology, cell biology and molecularbiology. AATBioquestoffers a rapidly expanding list of enabling products. Besidesthe standard catalog products, we also offer custom servicesto meet the distinct needsof each customer. Our current servicesinclude custom synthesisof biological detection probes,custom development of biochemical, cell-basedand diagnostic assaysandcustom high throughput screeningof drug discovery targets. It is my greatestpleasureto welcome you to AATBioquest.Wegreatly appreciate the constant support of our valuable customers.While we continue to rapidly expand, our core value remains the same: Innovation and Customer Satisfaction.We arecommitted to being the leading provider of novel biological detection solutions. Wepromise to extend thesevaluesto you during the courseof our serviceand to continue to support you with our new products and services.It is our greatesthonor to receivevaluable feedbacksand suggestionsfrom you so that we canbetter serveyour projects. Very truly yours, Zhenjun Diwu, Ph.D. President Tableof Contents GeneralInformation .............................................................................................................................................................................................. 2 Non-FluorescentCalciumSignaling Molecules& Chelators .............................................................................................................................. 5 NAADP....................................................................................................................................................................................................................................................................5 NAADP-AM............................................................................................................................................................................................................................................................5 Fluorimetric cADP-RiboseAssay...................................................................................................................................................................................................................6 BAPTAAM ..............................................................................................................................................................................................................................................................6 EGTAAM.................................................................................................................................................................................................................................................................6 Pluronic®F-127 .....................................................................................................................................................................................................................................................7 Probenecid ...........................................................................................................................................................................................................................................................7 FluorescentSingleWavelength CalciumIndicators...............................................................................................................................................8 Blue-Green Fluorescent Calcium Indicators .............................................................................................................................................................................................9 Cal-500™............................................................................................................................................................................................................................................................9 Green Fluorescent Calcium Indicators............................................................................................................................................................................................................10 Traditional GreenFluorescent Calcium Indicators ...............................................................................................................................................................................10 Fluo-8®.................................................................................................................................................................................................................................................................10 Cal-520®...............................................................................................................................................................................................................................................................11 CalbryteTM 520 ..................................................................................................................................................................................................................................................12 Red Fluorescent Calcium Indicators .............................................................................................................................................................................................................13 Cal-590TM .............................................................................................................................................................................................................................................................13 CalbryteTM 590 ..................................................................................................................................................................................................................................................14 Cal-630TM .............................................................................................................................................................................................................................................................14 CalbryteTM 630 ..................................................................................................................................................................................................................................................15 Rhod-4TM .............................................................................................................................................................................................................................................................16 NIRFluorescent Calcium Indicators ...............................................................................................................................................................................................................17 Cal-670TM .............................................................................................................................................................................................................................................................17 Cal-770TM ............................................................................................................................................................................................................................................................17 FluorescentRatiometricCalciumIndicators........................................................................................................................................................ 18 BTC........................................................................................................................................................................................................................................................................18 Fura-2 ..................................................................................................................................................................................................................................................................18 Fura-8TM ..............................................................................................................................................................................................................................................................18 Indo-1 ................................................................................................................................................................................................................................................................18 Cal RedTMR525/650