An International Journal Life Science Journal Acta Zhengzhou University Overseas Edition ISSN 1097-8135 Volume 6 - Number 2 (Cumulated No. 17), June 15, 2009 Zhengzhou University Marsland Press 100 Science Road Zhengzhou, Henan 450001, China P.O. Box 21126, Lansing, Michigan 48909, the United States 525 Rockaway PKWY, #B44, Brooklyn, New York 11212, the United States http://lsj.zzu.edu.cn http://www.sciencepub.net [email protected] [email protected] [email protected] 86-371-6778-1272 347-321-7172 http://lsj.zzu.edu.cn http://www.sciencepub.net Life Science Journal, Vol 6, No 3, 2009 (ISSN 1097-8135) http://lsj.zzu.edu.cn ; http://www.sciencepub.net Life Science Journal Life Science Journal is an official publication of The covers of Life Science Journal feature the Zhengzhou University, as the Acta illustrations the Editor-in-Chief selects from the Zhengzhou University Overseas Edition. The articles scheduled for publication in that issue. journal is published by Zhengzhou University Authors whose articles are chosen for a cover associated with American Marsland Press, with feature will be asked to provide a high-quality the international serial number of ISSN version of the selected illustration as well as a 1097-8135. This journal seeks to publish the best brief legend (four to five sentences) describing available scholarship on all aspects of life the significance of the image. In light of the science. As an international journal published rapid production schedule for journal covers, both in print and on internet, Life Science authors are expected to provide the requested Journal is dedicated to the dissemination of material within 3 days fundamental knowledge in all areas of life science. The main purpose of Life Science 3. Manuscripts submission Journal is to enhance our knowledge spreading in the world under the free publication principle. (1) Submission Methods: Electronic submission It publishes full-length papers (original through email and on line submission is required. contributions), reviews, rapid communications, (2) Font: Normal, Times New Roman, 10 pt, and any debates and opinions in all the fields of single space. nature and science. See Instructions for Authors (3) Indent: Type 4 spaces in the beginning of for manuscript submission information. each new paragraph. (4) Footnotes: In most instances information Editor-in-Chief : Shen, Changyu, Ph.D., should be presented in the text, not in footnotes Zhengzhou University, China and Header and Footer. Associate Editors-in-Chief : Ma Hongba, (5) Cover Page: Put detail information of authors Cherng Shen, Xin Shijun and a short title in the cover page. Editors: An Xiuli, George Chen, Dong Ziming, (6) Title: Use Title Case in the title and subtitles, Guangcai Duan, Jingjing Edmondson, Li Xinhua, e.g. “Debt and Agency Costs”. Li Yuhua, Mark Lindley, Liu Hongmin, Qi (7) Figures and Tables: Use full word of figure Yuanming, Shang Fude, Song Chunpeng, Sun and table, e.g. “Figure 1. Annul Income of Yingpu, Wang Lidong, Wen Jianguo, Xu Different Groups”, Table 1. Annual Increase of Cunshuan, Xue Changgui, Zhang Jianying Investment”. Zhang Kehao, Zhang Shengjun, Zhang Xueguo, (8) References: Number the references in the Zhang Zhan, Zhang Zhao, Zhu, Huaijie order of their first mention in the text.References Web Design: Jenny Young should include all the authors's last name and intials, title, journal, year, volume, issues, and pages, etc. Instructions for Authors Reference Examples: 1. Categories of publication Journal Article: Hacker J, Hentschel U, Dobrindt U. Prokaryotic chromosomes and disease. Research articles, reviews, objective descriptions, Science 2003;301(34):790-3. research reports, opinions/debates, news, letters Book: Berkowitz BA, Katzung BG. Basic and to editor, meeting report. clinical evaluation of new drugs. In: Katzung BG, ed. Basic and clinical pharmacology. 2. Cover feature Appleton & Lance Publisher. Norwalk, Connecticut, USA. 1995:60-9. Life Science Journal, Vol 6, No 3, 2009 (ISSN 1097-8135) http://lsj.zzu.edu.cn ; http://www.sciencepub.net 4. Manuscript preparation 5. Submission fee Each manuscript is suggested to include the US$50 per printed page of an article to defray following components but authors can do their costs of the publication will be paid by the own ways: authors when it is accepted. Extra expense for (1) Title : including the complete article title; color reproduction of figures will be paid by each author’s full name; institution(s) with authors (estimate of cost will be provided by the which each author is affiliated publisher for the author’s approval). (2) Abstract: including Background, Materials and Methods, Results, and Discussions. 6. Distributions (3) Key Words. On the title page, provide three to five key words identifying the subject of your Web version of the journal is freely opened to article. the world without payment or registration. The (4) Abbreviations. List all the abbreviations journal will be distributed to the selected needed here, so that they could be used directly libraries and institutions for free. US$100/issue in article. hard copy is charged for the subscription of (5) Introduction. other readers. (6) Materials and Methods. (7) Results. 7. Copyright and permissions (8) Discussions: Explain your interpretation of the data, especially compared with previously No part of this publication may be reproduced, published material cited in the References. stored in a retrieval system in any form or by (9) Acknowledgments. List the people indirectly any means, electronic, electrostatic, magnetic involved with the research to whom you may tape, mechanical, photocopying, recording or wish to give thanks. Also, current addresses of otherwise for commercial use, without authors (if they differ from those in the permission in writing from the copying holder. affiliation line) should be included here. (10) Correspondence. Correspondence author’s full name, institution, city state/province, zip code, country, telephone number, facsimile number (if available),and email address are listed here. (10) References: Authors are responsible for accuracy of citations. References must be limited to directly pertinent published works or papers that have been accepted for publication. Life Science Journal, Vol 6, No 2, 2009 http://lsj.zzu.edu.cn CONTENTS 1. The artificial microRNA mediates GUS-GFP gene silencing using 1 – 7 ath-miR169d precursor as backbone Chong Liu, Lan Zhang, Jie Sun, Yanzhong Luo, Mingbo Wang, Yunliu Fan, Lei Wang 2. Alkaline protease production by immobilized cells of Bacillus pumilis MTCC 2296 8 – 10 in various matrices Deepika Kumari, Neetu Sharma, Gulab Pandove, Varenyam Acha 3. AFLP analysis of genetic relationships and diversity of some Chinese 11 – 16 Osmanthus fragrans cultivars Xueyan Yan, Baolin Xiao, Yuanji Han, Wangjun Yuan, Fude Shang 4. Development of core collection using morphological descriptors in Sweet osmanthus 17 – 22 (Osmanthus fragrans Lour.) germplasm Wangjun Yuan, Jinsheng Lei, Yuanji Han, Xueyan Yan, Fude Shang 5. Enhancement of enzyme cytotoxicity mediated by HIV-1 TAT protein with Gly4 linker in vitro: 23 – 28 a study with TAT-TK fusion construct Zhe Wang, Zujiang Yu, Quancheng Kan, Jie Zhao, Heqing Jiang, Xiaofei Li 6. Randomness-induced evolution of the first-order to the second-order phase transition 29 – 32 in two-dimensional six-state potts model Chiachi Shih, Shingmin Hu, Muhsin Chen, Shuanyu Huang 7. Pulmonary functions and blood biochemical markers for workers with and without 33 – 39 coal worker pneumoconiosis Qinghan Jin, Ailin Liu, Qinghai Li, Shaohua Xie, Enguang Wan, Shaohui Zhang, Yinfeng Tan, Xiaofeng Li, Hong Xie, Wenqing Lu 8. Study of the influence of environmental tobacco smoke to trachea and lung of the animal model 40 – 42 Shuling Wang, Tianqi Wang, Shen Cherng 9. Clinical efficacy study of pelvic floor electrical stimulation for idiopathic detrusor overactivity 43 – 47 and urodynamic stress incontinence Huifan Liu, Qingwei Wang, Yan Qi, Ruili Zhang, Shuqiang Zuo, Xiaojin Wang, Jinxing Wei, Jianguo Wen · I · Life Science Journal, Vol 6, No 2, 2009 http://lsj.zzu.edu.cn 10. Expression of androgen receptor mRNA affected by the functions of lung and trachea in 48 – 50 animal model of Kunming mouse Shuling Wang, Xiufang Chen 11. Study of plasma levels of ApoA-I and ApoB for prognosis of acute ischemic stroke 51 – 56 Zhuo Li,Yuming Xu, Song Tan, Bo Song, Ming Liu 12. Somatic embryogenesis and in vitro regeneration of an endangered medicinal plant sarpgandha 57 – 62 (Rauvolfia serpentina L.) Prabhat Singh, Anand Singh, Arvind K. Shukla, Lalit Singh, Veena Pande, Tapan K. Nailwal 13. Study of the risk factors of postoperative upper gastrointestinal bleeding of percutaneous coro- 63 – 64 nary interventional therapy Zhenxiang Zhang, Wei Zheng, Junling Li 14. The effect of high frequency stimulation on intracellular Ca2+ in sympathetic PC12 cells 65 – 70 Yan Wo, Rong Xia, Feng Li, Wenlong Ding 15. Study on phylogenetic relationship of freshwater planarians (Turbellaria: Tricladida: Paludi- 71 – 75 cola) in nine Chinese localities using RAPD method Hecai Zhang, Guangwen Chen, Xiaojuan Sun, Cunshuan Xu 16. Therapeutic effect of L-carnitine on sialic acid, soluble Fas (sFas) and other biochemical vari- 76 – 82 ables in hyperinsulinemic rats Mohamed H. Mahfouz, Hala M. Ghanem, Mona A. Mohamed 17. Using technique of Video-Assistant Thoracic Surgery (VATS) and small incision for diagnosis 83 – 87 and treatment of pleurapulmonary diseases under local anesthesia