H. Reis et al: Differential proteomic and tissue expression analyses identify valuable diagnostic biomarkers of hepatocellular differentiation and hepatoid adenocarcinomas Suppl ementary Table 1) Immunohistochemical protocol and antibody information Antibody Company Clone/# Clonality Dilution Incubation Antigen retrieval Detection ABAT Abcam EPR4433 mono 1/3000 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP ACAA2 Abcam EPR6733 mono 1/100 30 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP ACADM Abcam EPR3708 mono 1/3000 30 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP ADH1B Abcam 4F12 mono 1/12.000 30 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP Arginase1 Abcam EPR6672(B) mono 1/1000 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP BHMT Abcam EPR6782 mono 1/100 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP FABP1 Acris AIV\09011PU-S mono 1/15.000 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP HAOX1 Acris AP18044PU-N poly 1/100 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP HepPar1 Dako OCH1E5 mono 1/800 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP HMGCS2 Abcam Ab104807 poly 1/50 60 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP H. Reis et al: Differential proteomic and tissue expression analyses identify valuable diagnostic biomarkers of hepatocellular differentiation and hepatoid adenocarcinomas Supplementary Table 2) Composition of the non-liver tumor TMA Diagnosis n % ADC 11 2.9 ADC Adenocarcinoma of the lung Carc. 9 2.3 Carc. Carcinoid CCRCC 10 2.6 CCRCC Clear cell renal cell carinoma CHRCC 9 2.3 CHRCC Chromophobe renal cell carcinoma CRC 27 7.0 CRC Colorectal adenocarcinoma ECU 17 4.4 ECU Endometroid adenocarcinoma of the uterus ENT 23 6.0 ENT Squamous cell carcinoma of the ear/nose/throat-region FTC 19 5.0 FTC Follicular thyroid carcinoma GAC 19 5.0 GAC Gastric adenocarcinoma IDC 17 4.4 IDC Invasive ductal carcinoma of the mamma ILC 17 4.4 ILC Invasive lobular carcinoma of the mamma MM 16 4.2 MM Malignant Melanoma MNS 17 4.4 MNS Mixed nonseminomatous germ cell tumors of the testis MTC 1 0.3 MTC Medullary thyroid carcinoma PCA 17 4.4 PCA Acinar adenocarcinoma of the prostate PDAC 31 8.1 PDAC Pancreatic ductal adenocarcinoma PTC 19 5.0 PTC Papillary thyroid carcinoma SCC 15 3.9 SCC Squamous cell carcinoma of the lung SCLC 11 2.9 SCLC Small cell lung carcinoma Sem. 19 5.0 Sem. Seminoma of the testis SOC 17 4.4 SOC Serous carcinoma of the ovary TCC 42 11.0 TCC Urothelial carcinoma Total 383 100.0 H. Reis et al: Differential proteomic and tissue expression analyses identify valuable diagnostic biomarkers of hepatocellular differentiation and hepatoid adenocarcinomas Supplementary Table 3) Clinicopathological data of the cohort with hepatoid carcinomas ID sex age primary metastasis AFP (IU/ml) HepPar1 Arginase1 CEA AFP GPC3 CK7 CK20 CK18 1 male 70 n/a lymph node 0,9 + - + - - + + + 2 male 38 gastric liver 23179 - - + - - - - + 3 male 72 pulmonary bone n/a - - - - - + (+) + 4 male 38 gastric liver n/a + - + - - - - + 5 male 78 n/a bone 2,8 - - - - - - - + 6 female 45 gastric bone n/a + - + - - + - + 7 male 73 gastric no 15041 + + - + + - - + 8 female 48 ovary abdominal wall n/a - + - + + (+) (+) + 9 female 67 n/a liver 2,4 + - - - - (+) - + 10 female 49 n/a ovary 26407 - + - + + - - + 11 male 69 n/a no n/a + - + - - + - + 12 male 53 colorectal liver n/a - - + - - + (+) + 13 female 49 pulmonary liver n/a + + + - - + - + AFP: Alpha-fetoprotein, IHC: Immunohistochemistry, HepPar1: hepatocyte paraffin 1, CEA: carcinoembryogenic antigen, GPC3: Glypican 3, CK7: Cytokeratin 7, CK20: Cytokeratin 20, CK18: Cytokeratin 18, n/a: not available, +: positive, (+): only few cells positive, -: negative .