Mouse and Rat Monoclonal Antibodies 2021 1 CNIO Monoclonal Antibodies Unit The Monoclonal Antibodies (mAb) Unit provides CNIO Research Groups with the “à la carte”, generation of mAbs which can then be used as tools to characterise new pathways involved in cancer development. We are highly specialised in mouse and rat monoclonal antibodies production. The Unit also offers mAbs production in gene- inactivated mice, mAb characterisation and validation, medium-scale mAb production and a service of Mycoplasma testing for the cell culture facility. The Unit is highly specialised in the characterisation and validation of antibodies. The mAbs generated by the Unit are extensively validated using a large set of tissue samples (specifically designed tissue-microarrays) and cell lines. After validation the mAbs are then tested in several applications. This work helps CNIO investigators save valuable research funds and effort, providing reliable reagents for use in their research projects. Techniques Available - Monoclonal antibodies production in mice - Monoclonal antibodies production in gene-inactivated mice - Rat monoclonal antibodies - Antibody characterization - Development of double and triple immunostaining techniques - Mycoplasma testing - Genetic immunization Monoclonal Antibodies Staff Unit Leader Giovanna Roncador ([email protected]) Technicians Lorena Maestre ([email protected]) Ana Isabel Reyes ([email protected]) Sherezade Jiménez ([email protected]) Alvaro García ([email protected]) Secretary Celia Ramos ([email protected]) mAbs Datasheet are available at: https://www.cnio.es/investigacion-e-innovacion/servicios/anticuerpos-monoclonales/ 2 INDEX AID .................................................................................................... 5 IgG (Fc specific) ........................................................................ 46 A-myb/B-myb/C-myb ............................................................... 5 KLH ................................................................................................. 48 ANX4 ............................................................................................... 6 KLHL-6 .......................................................................................... 49 BCL-2 ............................................................................................... 7 LCN-2............................................................................................. 50 BCL-6 .............................................................................................. 8 LKB1 ............................................................................................... 51 BCL-6 (Mouse specific) ............................................................. 9 LMO2 ............................................................................................. 52 BCL7A ........................................................................................... 10 Luciferase ..................................................................................... 52 Beta-Galactosidase .................................................................. 11 MALT-1 ........................................................................................... 53 BPTF .............................................................................................. 11 MAP-17 .......................................................................................... 54 BSA ................................................................................................ 12 MASTL ........................................................................................... 55 BUB-1 (Xenopus specific) ....................................................... 12 MBP ................................................................................................ 56 Cas9 (S. Pyogenes) .................................................................. 13 MCAK (Xenopus) ....................................................................... 56 CD115 (mouse specific) (CSF1R) ......................................... 22 MCRS1 (Microspherule protein 1) ......................................... 57 CD115 (CSF1R) ............................................................................. 21 MNDA ............................................................................................ 58 CD138 (Syndecan-1).................................................................. 22 NANOG (Mouse specific) ....................................................... 59 CD147 (Basigin) .......................................................................... 23 NKX2-3 .......................................................................................... 60 CD15 (FUT4) ................................................................................ 15 NOMO............................................................................................ 59 CD162 (PSGL1) ............................................................................ 24 NSE2 (NSMCE2) ........................................................................ 61 CD1d (CD1) ................................................................................... 13 p15 (CDKN2B) (Mouse specific) ........................................... 62 CD20 (Mouse specific) ............................................................ 16 p15/p16 .......................................................................................... 64 CD229 (Ly-9) .............................................................................. 25 p16 (CDKN2A) (Mouse specific) .......................................... 63 CD271 (NGFR) ............................................................................. 25 p19 (CDKN2A-ARF) (Mouse specific) ................................ 64 CD271 (NGFR) (mouse specific) .......................................... 26 p21 (Mouse specific) ................................................................. 65 CD272 (BTLA) ............................................................................. 27 p27(Mouse specific) ................................................................. 66 CD279 (PD1) ................................................................................ 28 p53 (Mouse specific) ................................................................ 66 CD30 (TNFRSF8) ...................................................................... 17 PDGF-β.......................................................................................... 67 CD370 (CLEC9A) ...................................................................... 29 PIM2 ............................................................................................... 68 CD38 (Cyclic ADP ribose hydrolase) .................................. 18 PLK1 ................................................................................................ 69 CD43 (Leukosialin) .................................................................... 18 PLK5 ............................................................................................... 69 CD63 (lamp-3) ............................................................................ 19 Poly PR ......................................................................................... 72 CD85A (LILRB3) ........................................................................ 19 POT1a ............................................................................................. 70 CD85A/B (LILRB3/LILRA6) ................................................... 20 PRDM1/Blimp-1 ........................................................................... 71 CD8a .............................................................................................. 14 PRIMPOL ...................................................................................... 73 CD8a (Mouse specific) ............................................................ 15 PSF1 ................................................................................................ 73 CDK6 ............................................................................................. 30 PSF2 ............................................................................................... 74 CENP-C1 ....................................................................................... 30 PSF3 ............................................................................................... 74 C-Myb ............................................................................................ 31 RAF1 ............................................................................................... 75 CSF1 ................................................................................................ 31 RTN4 .............................................................................................. 76 CTCF .............................................................................................. 32 SA-1 (STAG1) ............................................................................... 76 E2F1 ................................................................................................ 32 Saa3 (Mouse specific) .............................................................. 77 E4F1 ................................................................................................ 33 SPIB ................................................................................................ 78 EED ................................................................................................ 34