OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AP26398PU-N Polyclonal Antibody to CBLL1 / RNF188 - Purified Alternate names: Casitas B-lineage lymphoma-transforming sequence-like protein 1, E3 ubiquitin- protein ligase Hakai, HAKAI, RING finger protein 188, c-Cbl-like protein 1 Quantity: 0.1 mg Concentration: 0.1 mg/ml Background: Hakai is an E-cadherin binding protein, which has been identified as an E3 ubiquitin- ligase. Hakai contains SH2, RING, zinc-finger and proline-rich domains. Hakai interacts with E-cadherin in a tyrosine phosphorylation-dependent manner, inducing ubiquitination of the E-cadherin complex. Expression of Hakai in epithelial cells disrupts cell-cell contacts and enhances endocytosis of E-cadherin and cell motility. By promoting the endocytosis and dynamic recycling or destruction of E-cadherin complexes, Hakai is able to control epithelial-mesenchymal transitions under physiological and pathological conditions. Uniprot ID: Q75N03 NCBI: NP_079090.2 GeneID: 79872 Host / Isotype: Rabbit / IgG Format: State: Liquid 0.2 µm filtered Ig fraction Purification: Protein A Buffer System: PBS Preservatives: 0.02% sodium azide Stabilizers: 0.1% bovine serum albumin Applications: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50. Immunoflourescence: The typical starting working dilution is 1:50. Western blot: The typical starting working dilution is 1:50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody reacts with human Hakai, a c-Cbl-like protein. Species Reactivity: Tested: Human Storage: Store at 2 - 8 °C. Shelf life: one year from despatch. General Readings: 1. Fujita Y, Krause G, Scheffner M, Zechner D, Leddy HE, Behrens J, et al. Hakai, a c-Cbl- like protein, ubiquitinates and induces endocytosis of the E-cadherin complex. Nat Cell Biol. 2002 Mar;4(3):222-31. PubMed PMID: 11836526. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130514 1 / 1 Powered by TCPDF (www.tcpdf.org).