GEMINI GEMINI COMBO Compact Microplate Processor 350 Effective (geltend) / Review am: 02.10.2032 - stuff 18.10.2017 09:09:14 Instructions for use DMS Signature Authentication Code: 7fc3b1f5-dca2-4d01-9e52-f134b5141473 / DMS Document Id: [P000795646] / Version 1.0 / Page 1 of 425 Edition: Date: September 2017 Part No.: 15100013290 Revision: 10 Software: GEMINI / GEMINI COMBO: User Software from Version 2.03 Original Document 350 Effective (geltend) / Review am: 02.10.2032 - stuff 18.10.2017 09:09:14 We reserve the right to make changes in the course of technical development without previous notice. Copyright © 2017, STRATEC Biomedical AG. All rights reserved. STRATEC Biomedical AG Gewerbestraße 37 75217 Birkenfeld, GERMANY Neither this manual nor any parts of it may be duplicated or transmitted in any way without the written approval of STRATEC Biomedical AG. DMS Signature Authentication Code: 7fc3b1f5-dca2-4d01-9e52-f134b5141473 / DMS Document Id: [P000795646] / Version 1.0 / Page 2 of 425 Known Limitations KL Known Limitations Load: Plates If the order of plates within a worklist is changed, the order of the wash buffer bottles and Bottles can change as well. Always check for the correct positioning of the wash buffer Order bottles before starting the worklist from the load dialog. Manual assigned wash buffer positions or blind reagent positions (in the dilution areas) have to be checked carefully before starting the worklist by pressing the "Start" button of the Load window. Assigned positions can change if the order of the plates has been changed by "Edit Panel". Loading Bay In case a pipettor error occurred, do not remove any racks from the instrument even if the LED is flashing. If this rack is still required the accurate tracking might not be working exact anymore. Movable After a crash of the movable scanner with an obstacle (and the option "Abort" is Barcode selected), consecutive move problems can occur. Turn off the scanner and then set Scanner the scanner focus on the required lane. In case a positioning error of the scanner unit occurs after a crash, the button "Abort worklist" in this case only aborts the current step and actually follows an "Abort" logic. Unload Plate If a plate in a larger worklist is aborted during a shake step on the plate transport, wait to unload the plate until the shaking is finished. 350 Effective (geltend) / Review am: 02.10.2032 - stuff 18.10.2017 09:09:14 Gemini - Instructions for use manual - Rev. 10 KL-1 DMS Signature Authentication Code: 7fc3b1f5-dca2-4d01-9e52-f134b5141473 / DMS Document Id: [P000795646] / Version 1.0 / Page 3 of 425 Known Limitations Intentionally left blank. 350 Effective (geltend) / Review am: 02.10.2032 - stuff 18.10.2017 09:09:14 KL-2 Gemini - Instructions for use manual - Rev. 10 DMS Signature Authentication Code: 7fc3b1f5-dca2-4d01-9e52-f134b5141473 / DMS Document Id: [P000795646] / Version 1.0 / Page 4 of 425 Table of Contents KL Known Limitations. KL-1 1 Introduction . 1-1 1.1 Intended Use . 1-1 1.1.1 Operator Requirements . 1-2 1.1.2 Good Laboratory Practice. 1-2 1.1.3 Limited Warranty. 1-2 1.2 Typographical Conventions . 1-3 1.2.1 Display of Warnings and Notes. 1-3 1.2.2 Used Warning Symbols . 1-4 1.2.3 Other Symbols . 1-5 1.2.4 Special Types . 1-5 1.3 Safety Instructions. 1-6 1.3.1 General Safety . 1-6 1.3.2 Electrical Safety . 1-8 1.3.3 Laser Safety . 1-9 1.3.4 Mechanical Safety . 1-10 1.3.5 Biological Safety . 1-11 1.4 Positions of Safety Labels and Type Label. 1-12 1.4.1 General Warning Labels . 1-12 1.4.2 Biological Hazard Labels . 1-12 1.4.3 Electrical Hazard Labels . 1-12 1.4.4 Laser Hazard Labels. 1-12 1.4.5 Cut Injury Hazard Labels . 1-13 1.4.6 Type Label . 1-13 1.5 Radio Interferences . 1-14 1.6 Abbreviations. 1-15 2 Instrument Description . 2-1 2.1 Instrument Overview . 2-2 2.1.1 Instrument. 2-2 2.1.2 Liquid Connections . 2-5 2.1.3 Electrical Connections . 2-7 2.2 Use of the Modules . 2-8 2.2.1 Microplates in the Plate Transport . 2-8 350 Effective (geltend) / Review am: 02.10.2032 - stuff 18.10.2017 09:09:14 2.2.2 Disposable Tip Racks . 2-10 2.2.3 Dilution Plates, Archive Plates, and Large Reagent Bottles . 2-11 2.2.4 Loading Bay for Samples and Reagents . 2-14 2.2.5 Washer and Wash Buffers . 2-18 2.2.6 Waste Container . 2-18 2.2.7 Incubator and Stacker. 2-19 2.2.8 Photometer . 2-19 2.2.9 Pipettor and Diluter Pump. 2-20 2.2.10Touch Screen . 2-22 2.2.11IFA Bay, IFA Trays and IFA Slides (optional). 2-23 2.3 Accessories and Consumables . 2-25 2.4 Principles of Methods . 2-26 2.4.1 Absorbance Photometry . 2-26 2.4.2 Bichromatic Measurement . 2-26 Gemini - Instructions for use manual - Rev. 10 TOC-1 DMS Signature Authentication Code: 7fc3b1f5-dca2-4d01-9e52-f134b5141473 / DMS Document Id: [P000795646] / Version 1.0 / Page 5 of 425 3 Basic Functions . 3-1 3.1 Menus and Symbols . 3-1 3.2 Open/Load . 3-10 3.3 Save . 3-11 3.4 Print on the Printer . 3-13 3.4.1 Print . 3-13 3.4.2 Print Setup . 3-14 3.4.3 Print Preview . 3-15 3.5 Online Keyboard . 3-16 3.6 Selection Dialog . 3-16 4 Use of the Instrument . 4-1 4.1 Safety and Hints . 4-1 4.2 Brief Sequence Plan . 4-2 4.3 Start-up . 4-3 4.3.1 Registered Users . 4-4 4.3.2 Unknown User Name / Unregistered Users. 4-4 4.3.3 First-Time Use (Password Registration) . 4-5 4.3.4 Incorrect Password . 4-6 4.3.5 Successive Users . 4-6 4.3.6 Users with Restricted Access Rights . 4-7 4.4 Load Samples and Assign Assays . 4-8 4.4.1 Load Samples . 4-8 4.4.2 Assign Assays to the Samples (Tabular Sample Editor). 4-10 4.4.3 Import Sample Data and Linked Assays through Host Connection4- 13 4.5 Create a Worklist. 4-14 4.6 Lot Specific Values . 4-15 4.7 The Worklist Window . 4-18 4.7.1 Worklist Parameters. 4-20 4.7.2 Schedule . 4-21 4.7.3 Plate Layouts . 4-23 4.7.4 Reagent Requirements . 4-24 4.7.5 System Status . 4-26 4.7.6 Active Event Log . 4-27 4.7.7 Job List . 4-30 4.7.8 Sample Archiving Information . 4-30 4.8 Start Worklist. 4-31 4.8.1 Load.