200512 17 MIX 1 2 - CH3 100000 13 7 14 18 50000 Intensity[µV] 6 9 4 8 10 11 12 2 3 5 1 15 16 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig. S1A. Example of a chromatogram for the anthocyanin standards used in this study. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin-3-O-galactoside, (4) delphinidin-3-glucoside, (5) cyanidin-3-O-galactoside, (6) malvidin-3,5- diglucoside; (7) delphinidin-3-O-rutinoside, (8) Cyanidin-3-glucoside, (9) cyanidin-3-O-rutinosoide, (10) cyanidin-3-O-arabinose, (11) pelargonidin-3-glucoside, (12) peonidin-3-O-galactoside, (13) pelargonidin, (14) malvidin-3-O-galactoside, (15) malvidin-3-glucoside, (16) peonidin-3-O-arabinoside, (17) delphinidin, (18) cyanidine. 200513 3b bilberry dil 20x - CH3 30000 5 2 8 7 4 13 20000 1 3 11 Intensity[µV] 6 10000 10 12 14 17 16 9 18 21 15 19 20 22 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig.S1B. Example of a chromatogram for bilberry extract before in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin- 3-O-galactoside, (4) delphinidin-3-glucoside, (5) cyanidin-3-O-galactoside, (6) malvidin-3,5-diglucoside; (7) delphinidin-3-O-rutinoside, (8) Cyanidin-3-glucoside, (9) cyanidin-3-O-rutinosoide, (10) cyanidin-3-O- arabinose, (11) pelargonidin-3-glucoside, (12) peonidin-3-O-galactoside, (13) pelargonidin, (14) malvidin- 3-O-galactoside, (15) malvidin-3-glucoside, (16) peonidin-3-O-arabinoside, (17) delphinidin, (18) cyanidine. 6 200512 in vitro dig 1C - CH3 14 40000 8 12 4 9 Intensity[µV] 20000 13 2 5 7 17 1 11 10 15 3 16 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig. S1C. Example of a chromatogram for bilberry extract after in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin-3-O- galactoside, (4) delphinidin-3-glucoside, (5) cyanidin-3-O-galactoside, (6) malvidin-3,5-diglucoside; (7) delphinidin-3-O-rutinoside, (8) Cyanidin-3-glucoside, (9) cyanidin-3-O-rutinosoide, (10) cyanidin-3-O- arabinose, (11) pelargonidin-3-glucoside, (12) peonidin-3-O-galactoside, (13) pelargonidin, (14) malvidin- 3-O-galactoside, (15) malvidin-3-glucoside, (16) peonidin-3-O-arabinoside, (17) delphinidin. 4 200513 2a blackcurrant dil 20x - CH3 40000 2 30000 20000 Intensity[µV] 10000 1 3 5 6 7 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig. S1D. Example of a chromatogram for blackcurrant extract before in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin-3-O-galactoside, (4) delphinidin-3-glucoside, (5) cyanidin-3-O-galactoside, (6) malvidin-3,5- diglucoside; (7) delphinidin-3-O-rutinoside. 4 200512 in vitro dig 3B - CH3 100000 2 Intensity[µV] 50000 1 3 5 7 6 8 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig. S1E. Example of a chromatogram for blackcurrant extract after in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin-3-O-galactoside, (4) delphinidin-3-glucoside, (5) cyanidin-3-O-galactoside, (6) malvidin-3,5- diglucoside; (7) delphinidin-3-O-rutinoside, (8) Cyanidin-3-glucoside. 1 2 3 4 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig.S1F. Example of a chromatogram for raspberry extract before in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin- 3-O-galactoside, (4) delphinidin-3-glucoside, (5) cyanidin-3-O-galactoside. 1 200512 in vitro dig 2B - CH3 10000 4 Intensity[µV] 2 5 3 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig. S1G. Example of a chromatogram for raspberry extract after in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin- 3-O-galactoside, (4) delphinidin-3-glucoside, (5) cyanidin-3-O-galactoside. 2 200513 1a Strawbweey dil 20x - CH3 20000 Intensity[µV] 10000 1 3 4 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig. S1H. Example of a chromatogram for strawberry extract before in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin-3-O-galactoside, (4) delphinidin-3-glucoside. 2 200512 in vitro dig 4A - CH3 40000 20000 Intensity[µV] 3 4 1 0 0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0 Retention Time [min] Fig. S1I. Example of a chromatogram for strawberry extract after in vitro digestion. Peaks correspond to the following anthocyanins: (1) cyanidin-3,5-diglucoside, (2) pelargonidin-3-O-rutinoside, (3) delphinidin- 3-O-galactoside, (4) delphinidin-3-glucoside. Table S1. Survival rate averages of S. cerevisiae SKQ2n treated with H2O2 (0.45 M) cultured in YNB with 2% glucose without berry extracts (Control), with berry extracts before in vitro digestion (before) and after in vitro digestion (After).a Berry Survival (%) SE Bilberry Control 17.40 def 5.92 Before 14.05 f 5.67 After 41.27 c 5.83 Blackcurrant Control 23.93 de 5.97 Before 15.33 ef 5.86 After 52.16 b 4.47 Raspberry Control 19.16 def 5.81 Before 15.84 ef 5.88 After 46.49 bc 6.22 Strawberry Control 25.78 d 5.42 Before 22.34 def 5.38 After 79.38 a 2.22 aSignificant difference between treatments were detected using the Tukey's Studentized Range (HSD) at the 5% level of significance. Averages followed by the same letters are not significantly different (n= 30; p <0.0001). aSE= Standard error. .