Umemiya‑Shirafuji et al. BMC Res Notes (2021) 14:326 https://doi.org/10.1186/s13104‑021‑05740‑3 BMC Research Notes DATA NOTE Open Access Data from expressed sequence tags from the organs and embryos of parthenogenetic Haemaphysalis longicornis Rika Umemiya‑Shirafuji1* , Jinlin Zhou1,2, Min Liao1, Badgar Battsetseg1,3, Damdinsuren Boldbaatar1,4, Takeshi Hatta1,5,6, Thasaneeya Kuboki1,7, Takeshi Sakaguchi1, Huey Shy Chee1, Takeharu Miyoshi5, Xiaohong Huang5, Naotoshi Tsuji5,6, Xuenan Xuan1 and Kozo Fujisaki1 Abstract Objectives: Haemaphysalis longicornis is the most important tick species in Japan and has a wide range of vector capacity. Due to its veterinary and medical importance, this tick species has been used as a model for tick/vector biological studies. To identify the key molecules associated with physiological processes during blood feeding and embryogenesis, full‑length cDNA libraries were constructed using the fat body, hemocytes‑containing hemolymph, midgut, ovary and salivary glands of fed females and embryos of the laboratory colony of parthenogenetic H. longi- cornis. The sequences of cDNA from the salivary glands had been already released. However, the related information is still poor, and the other expressed sequence tags have not yet been deposited. Data description: A total of 39,113 expressed sequence tags were obtained and deposited at the DNA DataBank of Japan. There were 7745 sequences from embryos, 7385 from the fat body, 8303 from the hemolymph including hemocytes, 7385 from the midgut, and 8295 from the ovary. The data, including expressed sequence tags from the salivary glands was summarized into Microsoft Excel fles. Sharing this data resource with the tick research community will be valuable for the identifcation of novel genes and advance the progress of tick research. Keywords: Tick, Haemaphysalis longicornis, Embryo, Fat body, Hemolymph, Midgut, Ovary, Salivary glands, Expressed sequence tags, Full‑length cDNA library Objective vaccines or therapeutic agents against T. orientalis infec- Haemaphysalis longicornis is an important tick species, tion available at present in Japan. H. longicornis also and is a vector for various pathogens afecting humans occurs in Australia, New Zealand, New Caledonia, the and animals in Asia and Oceania. In the veterinary feld, Fiji Islands, Korea, China and Russia [1]. Although the the tick species is a major pest of cattle, because it can tick species was not detected outside of quarantine until spread Teileria orientalis, a protozoan parasite, which 2017, a heavy infestation of H. longicornis was recently causes piroplasmosis and produces economic losses reported in the United States [2]. H. longicornis is a vec- to livestock industry producers. Tere are no anti-tick tor of not only bovine piroplasmosis, but also canine babesiosis caused by Babesia parasites, and rickettsiosis and viral diseases in humans. Troughout its distribu- *Correspondence: [email protected] 1 National Research Center for Protozoan Diseases, Obihiro University tion, H. longicornis is an increasing threat to livestock of Agriculture and Veterinary Medicine, Inada‑Cho, Obihiro, Hokkaido animals and humans. 080‑8555, Japan Haemaphysalis longicornis has been used as a model Full list of author information is available at the end of the article for tick/vector studies. As a development platform for © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/. The Creative Commons Public Domain Dedication waiver (http:// creat iveco mmons. org/ publi cdoma in/ zero/1. 0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Umemiya‑Shirafuji et al. BMC Res Notes (2021) 14:326 Page 2 of 4 novel control strategies, including anti-tick vaccines, hemocytes, midgut, and salivary glands were pooled for bio-acaricides and anti-protozoal drugs against ticks each organ. Te ovary samples were collected from both and tick-borne diseases, we have constructed full-length partially-engorged (four to fve-day-fed) and engorged cDNA libraries using laboratory-reared parthenogenetic female ticks, and pooled. Eggs laid at the third to fourth H. longicornis. Te expressed sequence tags (ESTs) in day after the onset of oviposition were collected and these libraries have made it possible to identify cDNA incubated at 28 °C for seven to eight days to develop. Te sequences which may be used to elucidate molecular samples were homogenized using a pestle in TRI reagent processes such as blood digestion, oxidative stress, apop- (Sigma-Aldrich, MO, USA). Total RNA extraction was tosis, reproduction, and survival [3–8]. Currently, only performed using TRI reagent, according to the manu- 8471 EST sequences of the salivary glands are available facturer’s protocol. cDNA was synthesized from 5 μg of in public databases [9]; information regarding the other total RNA using the G-Capping method [11], and ligated sequences has not been shared yet. Tis situation means into the plasmid vector pGCAP1. Te resulting plasmids that the extension and improvement of tick research is were transformed into Escherichia coli DH12S (Termo limited. Te data should be shared worldwide, because of Fisher Scientifc, MA, USA). A total of 10,000 recom- its veterinary and medical importance. binant transformants from the library were randomly selected for plasmid DNA purifcation and sequencing. Data description Te nucleotide sequences were determined using an Te full-length cDNA library was made using the vector- automated sequencer (ABI PRISM 310 Genetic Analyzer; capping method [11]. Te construction of each cDNA Termo Fisher Scientifc) and then analyzed for identity library has previously been reported [4, 5]. Te parthe- using the BLASTX program (National Center for Bio- nogenetic tick H. longicornis (Okayama strain) was main- technology Information (NCBI); https:// blast. ncbi. nlm. tained at the National Research Center for Protozoan nih. gov/ Blast. cgi). Te ESTs were constructed by random Diseases, Obihiro University of Agriculture and Veteri- partial sequencing of the 5’-terminal of the cDNA clones nary Medicine, and was fed on the ears of Japanese white from each cDNA library. rabbits (Japan SLC, Shizuoka, Japan) using the cotton A total of 39,113 ESTs obtained were deposited in bag method [10]. Female ticks which had been fed from the DNA DataBank of Japan (DDBJ) [12]. Te depos- three to four days (corresponding to the rapid feeding ited sequences contained 7745 ESTs from embryos stage) were dissected in cold phosphate-bufered saline (Table 1, Data fle 12) [13], 7385 from the fat body (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM (Table 1, Data fle 7) [14], 8303 from the hemolymph KH2PO4, pH 7.4), and the fat body, hemolymph including including hemocytes (Table 1, Data fle 8) [15], 7385 Table 1 Overview of data fles/data sets Label Name of data fle/data set File types Data repository and identifer (DOI or accession number) (fle extension) Data fle 1 ESTs_Hl_Fat Body MS Excel fle (.xlsx) Obihiro University Archives of Knowledge (http:// doi. org/ 10. 24556/ 00004 700) [18] Data fle 2 ESTs_Hl_Hemolymph MS Excel fle (.xlsx) Obihiro University Archives of Knowledge (http:// doi. org/ 10. 24556/ 00004 701) [19] Data fle 3 ESTs_Hl_Midgut MS Excel fle (.xlsx) Obihiro University Archives of Knowledge (http:// doi. org/ 10. 24556/ 00004 702) [20] Data fle 4 ESTs_Hl_Ovary MS Excel fle (.xlsx) Obihiro University Archives of Knowledge (http:// doi. org/ 10. 24556/ 00004 703) [21] Data fle 5 ESTs_Hl_Salivary glands MS Excel fle (.xlsx) Obihiro University Archives of Knowledge (http:// doi. org/ 10. 24556/ 00004 704) [22] Data fle 6 ESTs_Hl_Embryo MS Excel fle (.xlsx) Obihiro University Archives of Knowledge (http:// doi. org/ 10. 24556/ 00004 705) [23] Data fle 7 Hl FB full‑length cDNA library FASTA DDBJ/ENA/GenBank (Accession numbers: HY961648‑HY969032) https:// ident ifers. org/ ncbi/ insdc: HY961 648 [14] Data fle 8 Hl HE full‑length cDNA library FASTA DDBJ/ENA/GenBank (Accession numbers: HY969033‑HY977335) https:// ident ifers. org/ ncbi/ insdc: HY969 033 [15] Data fle 9 Hl MG full‑length cDNA library FASTA DDBJ/ENA/GenBank (Accession numbers: HY977336‑HY984720) https:// ident ifers. org/ ncbi/ insdc: HY977 336 [16] Data fle 10 Hl OV full‑length cDNA library FASTA DDBJ/ENA/GenBank (Accession numbers: HY984721‑HY993015) https:// ident ifers. org/ ncbi/ insdc: HY984 721 [17] Data fle 11 Hl Sg full‑length cDNA library FASTA DDBJ/ENA/GenBank (Accession numbers: DC574924‑DC583394) https:// ident ifers. org/ ncbi/ insdc: DC574 924 [9] Data fle 12 Hl EM full‑length cDNA library FASTA DDBJ/ENA/GenBank (Accession numbers: HY953903‑HY961647) https:// ident ifers. org/ ncbi/ insdc: HY953 903 [13] Umemiya‑Shirafuji et al. BMC Res Notes (2021) 14:326 Page 3 of 4 from the midgut (Table 1, Data fle 9) [16], and 8295 Abbreviations BLAST: Basic Local Alignment Search Tool; DDBJ: DNA DataBank of Japan; ENA: from the ovary (Table 1, Data fle 10) [17]. Sample European Nucleotide Archive; EST: Expressed sequence tag; NCBI: National information was deposited in the DDBJ BioSample Center for Biotechnology Information. database (Table 1, Data fles 7–10 and 12) [13–17].