EUCARPIA EUROPEAN ASSOCIATION FOR RESEARCH ON PLANT BREEDING XIIth Meeting on Genetics and Breeding of Capsicum and Eggplant Noordwijkerhout, The Netherlands May 17-19, 2004 Plantum NL Plant Research International 2 Correct reference: Proceedings of the XIIth EUCARPIA Meeting on Genetics and Breeding of Capsicum and Eggplant, May 17-19, 2004. Noordwijkerhout, The Netherlands. Editor: R.E. VOORRIPS Plant Research International Wageningen UR P.O. Box 16 6700 AA Wageningen The Netherlands 3 ORGANIZING COMMITTEE Jair Haanstra (Chairman), Harriette Aarden, Tom van der Heiden, Herman Koning, Jurko Leij, Clemens Stolk, Dick Vreugdenhil, Roeland Voorrips SCIENTIFIC COMMITTEE Roeland Voorrips, Jair Haanstra, Ietje Boukema (The Netherlands), Alain Palloix, Marie-Christine Daunay (France), Istvan Nagy (Hungary), Piero Belletti (Italy), Kazim Abak (Turkey), Paul Bosland (USA) ACKNOWLEDGEMENTS The meeting has been supported by the following institutions and companies: EUCARPIA Plantum NL Rijk Zwaan Plant Research International Seminis Vegetable Seeds Europe De Ruiter Seeds Syngenta Seeds Enza Zaden Western Seed Nunhems Naktuinbouw 4 FOREWORD This book contains the Proceedings of the XIIth Meeting on Genetics and Breeding of Capsicum and Eggplant, held on 17-19 May, 2004. Since the first meeting, held in Torino (Italy) in 1971, the rhythm of one meeting every three years has been steadily maintained, which is an indication of the continuing interest in our field. This book is the only published account of the meeting. It contains all the lectures and poster abstracts accepted by the scientific committee. The book was prepared before the meeting, in order to be a useful guide to the lectures and posters. Therefore, it may contain some lectures and posters that were not actually presented. All texts have been edited if necessary to conform to a uniform format. Occasionally evident typing and language errors have been corrected. Otherwise the texts are printed as submitted by the authors, who remain responsible for the published data and information. The lectures and posters are classified into seven sections: 1. Invited lectures 2. Genetic resources 3. Breeding programs and strategies 4. Physiology and quality 5. Diseases and resistance 6. Biotechnology 7. Genome analysis In each section the lectures appear first, followed by the poster abstracts in alphabetical order of first author. In comparison with previous meetings, the number of reports dealing with genomics is again increased. These reports can be found in several sections, as the relevance of genomics to other fields is becoming more evident. The section on Genome analysis is mostly devoted to new technologies and to genomics results that don’t fit well in other sections. We sincerely hope that this meeting will again stimulate the exchange of ideas and be an inspiration to breeders and scientists worldwide, working in the field of Capsicum and Eggplant breeding and genetics. Roeland Voorrips, on behalf of the Organizing and Scientific Committees 5 CONTENTS INVITED LECTURES..............................................................................................15 Plants ‘cry for help’: Opportunities for integrating plant breeding and biological control Dicke M....................................................................................................................16 Optimal methods of using marker information in gene introgression and gene pyramiding programs Frédéric Hospital, Bertrand Servin, Olivier C. Martin, Marc Mézard ...................24 GENETIC RESOURCES..........................................................................................31 Lectures The taxonomic answer to the species dilemma in Capsicum chinense and C. frutescens: a key issue for Capsicum improvement and conservation genetics Paul W. Bosland, Jit B. Baral..................................................................................32 Spanish traditional varieties of eggplant: diversity and interest for plant breeding J. Prohens, J. M. Blanca, A. Rodríguez-Burruezo, F. Nuez ....................................38 Assessment of genetic relationships in the genus Capsicum using different DNA marker systems Kochieva E.Z., Ryzhova N.N., van Dooijeweert W., Boukema I.W., Arens P.....................................................................................................................44 Posters Collection and Characterization of Capsicum annum (Sweet pepper) in Nigeria O.A. Adetula and J.O. Babatola ..............................................................................51 Genetic variability studies in Chilli (Capsicum annuum l.) N. Basavaraja, R.M. Hosamani and Krishna. C. Ukkund .......................................52 Characterization of different pepper genotypes collected from coastal regions of Turkey Davut Keles, Suleyman Karagul, Saadet Buyukalaca .............................................53 Genetic diversity among Capsicum chinense Jacq. accessions revealed by different molecular marker systems Kochieva E.Z., Ryzhova N.N., van Dooijeweert W., Boukema I.W., Arens P.....................................................................................................................54 Amplified fragment length polymorphism (AFLP) for genetic diversity assessment in Italian sweet pepper (Capsicum annuum L.) germplasm G. Nervo, E. Portis, L. Barchi and S. Lanteri..........................................................55 Genetic resources of eggplant (solanum melongena l.) and their utilization in India Mathura Rai, Major Singh and Sanjeet Kumar.......................................................56 6 Solanum melongena (Eggplant) - A possible interspecific origin V. Rattan Kumar, Y.V. Rao and B.G.S. Rao ............................................................57 Germplasm collection eggplant and pepper genetic resources in Armenia K.M. Sarikyan ..........................................................................................................58 Current state of the collection of pepper (Capsicum annuum L.) in Gene Bank Olomouc, RICP Prague Helena Stavělíková...................................................................................................59 The CGN Capsicum (Pepper) and non-tuber bearing Solanum (eggplant) collections Willem van Dooijeweert and Ietje Boukema............................................................60 Studies on electrophoresis on interspecific hybrids of eggplant Warade S. D., Sanap P.B., Patil R.S., Bhalekar M.N. and Gupta N.S...................61 Morphological evaluation of Capsicum germplasm using statistic analysis Young Chae, J-C Shieh, J-B Park and L.M. Engle.................................................62 Genetic resources of pepper (capsicum annuum l.) in Serbia B. Zecevic, M. Mijatovic, R. Djordjevic and D. Cvikic............................................63 BREEDING PROGRAMS AND STRATEGIES....................................................65 Lectures Performance of phenotypic selection for a polygenic trait : QTL conservation and loss across the cycles of selection of pepper for resistance to Phytophthora capsici Alain Palloix, Ganaëlle Bernard, Arnaud Thabuis, Anne-Marie Daubèze, Thérèze Phaly, Patrick Signoret, Véronique Lefebvre.............................66 Results of three cycles of marker-assisted backcross in pepper for resistance QTLs to Phytophthora capsici Véronique Lefebvre, Arnaud Thabuis, Bertrand Servin, Anne-Marie Daubèze, Patrick Signoret, Frédéric Hospital, Alain Palloix .................................73 Studies on interspecific hybridization in eggplant (Solanum melongena L.) for little leaf disease resistance S.D. Warade, P.B. Sanap, D.M. Sawant and N.S. Gupta ........................................79 Posters Evaluation of Chilli (capsicum annuum l.) genotypes for variability, path analysis and genetic divergence under high temperature M.N. Bhalekar, S.N. Dutonde, N.S. Gupta, S.D. Warade ........................................84 A new once-over harvest-type varieties 'Saeng-ryeog no. 211' and 'Saeng-ryeog no. 213' in red pepper (capsicum annuum L.) M.C. Cho, D.H. Pae, Y.S. Cho, Y. Chae, W.M. Lee, D.H. Kim, Y.H. Om, D.S. Kim, S.R. Cheong, I.G. Mok, J.Y. Yoon............................................................85 Genetic analysis of dry fruit yield, fruit quality and pest resistance in Chilli (Capsicum annuum L.) R.C. Jagadeesha, N. Basavaraja, Ravi Hunje .........................................................86 7 Earliness as an important trait of field grown pepper (Capsicum annuum L.) in Central and Northern Europe A. Korzeniewska and K. Niemirowicz-Szczytt..........................................................87 Male sterility research at iivr and a success story with ms-10 gene in hot pepper (Capsicum annuum l.) Sanjeet Kumar, Major Singh and Mathura Rai.......................................................88 Capsicum: cost effective hybrid seed production N. Nagy, N.F. Derera and P.J. Sharp......................................................................89 Genetic divergence and development of hybrids in bell pepper Nandadevi and P. R. Dharmatti...............................................................................90 Development of eggplant functional male sterile lines and its utilization Tian Shibing, Wang Yongqing, Liu Fuzhong, Luo Zhangyong, Pi Wei, Chen Yikang, Liu Junshao........................................................................................91 Combining ability analysis of Eggplant functional male sterile lines and restorers