Center for Inherited Disorder Services Offered of Energy Metabolism (CIDEM) __________________________________ Wearn Bldg., Room 649 Human Clinical Samples University Hospitals Case Medical Center 11100 Euclid Avenue, Cleveland OH 44106 CIDEM Quantitative Analysis Phospholipid Analysis (including cardiolipin and Tel: 216-844-1286 monolysocardiolipin molecular species) Fax:216-844-8005 Center for Inherited Isolated Skeletal Muscle Mitochondria [email protected] Disorders of Energy Cardiolipin and Monolysocardiolipin Analysis www.case.edu/med/CIDEM/ Skeletal Muscle Metabolism Skin Fibroblasts Tax ID: 34-1720429 CLIA: 36D0680824 ww.case.edu/med/CIDEM Available Fall 2012! CLIA: 36D0925804 Quantitative Analysis NPI: 1578706438 Phospholipid Analysis (including cardiolipin and monolysocardiolipin molecular species) Quantitative Skeletal Muscle (frozen/fresh) Isolated Human Skeletal Muscle Mitochondria Fibroblasts Reference Intervals: Box = Mean ±SD; Whiskers = Range Blood Phospholipid Analysis 300 1.4 ___________________________________ n 250 1.2 by HPLC-MS 1 protei 200 Team Members 0.8 mito 150 Charles Hoppel, MD 0.6 Juhnwan Kim, PhD 100 0.4 Paul Minkler 50 nmol/mg 0.2 0 0 CL PE PC PI PG Quality Control Results for Cardiolipin Center for Mitochondrial Diseases Rat Skeletal Muscle Mitochondria, N = 48, 11% RSD Case Western Reserve University School of Medicine Department of Pharmacology 10900 Euclid Avenue 70 Cleveland, Ohio 44106-4981 60 Center for Mitochondrial Diseases Among the nation’s leading academic medical cen- Case Western Reserve University School of Medicine 50 ters, University Hospitals Case Medical Center is the 40 Department of Pharmacology primary affiliate of Case Western Reserve University 30 10900 Euclid Avenue School of Medicine, a nationally recognized leader in Cleveland, Ohio 44106-4981 20 medical research and education. 10 0 2011/ 07 2012/ 06 Human Skeletal Muscle Mitochondria Human Skin Fibroblasts Human Skeletal Muscle Phospholipid HPLC-MS Chromatogram and Spectra Phospholipid HPLC-MS Chromatogram and Spectra Phospholipid HPLC-MS Chromatogram and Spectra 747.5 766.7 775.7 766.7 766.7 747.6 885.8 788.6 885.8 885.8 599.4 836.5 1452.3 1448.1 1185.7 1163.4 1448.0 786.7 804.7 747.7 1185.7 802.9 802.8 O O OH Symbol Key: O 540.3 O 747.6 O PE - phosphatidylethanolamine monohydroperoxides PG - phosphatidylglycerol Oxidized PI - phosphatidylinositol Cardiolipin O OO H OO H O LPI - lysophosphatidylinositol Reversed- O PS - phosphatidylserine O Phase Ion-Pair dihydroperoxides CL - cardiolipin MLCL - monolysocardiolipin HPLC-MS/MS O O OH O PC - phosphatidylcholine O O OH SM - sphingomyelin O bis monohydroperoxides LPC - lysophosphatidylcholine .