(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/037361 Al 01 March 2018 (01.03.2018) W !P O PCT (51) International Patent Classification: Published: A 31/04 (2006.01) A01N 35/08 (2006.01) — with international search report (Art. 21(3)) A0 43/16 (2006.01) A01N 25/02 (2006.01) A01N 65/00 (2009.01) A01N 25/30 (2006.01) A01N 65/08 (2009.01) A01P 7/02 (2006.01) (21) International Application Number: PCT/IB20 17/055094 (22) International Filing Date: 23 August 2017 (23.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/378,259 23 August 2016 (23.08.2016) US (71) Applicant: ECOFLORA AGRO SAS [CO/CO]; Mall In diana Center, Oficina 279, Via Medellin-Rionegro, Rio N e gro, Alto de las Palmas (CO). (72) Inventors: JIMENEZ MARTINEZ, James; Calle 27D sur #27C-5 1, Medellin, Antioquia (CO). GIL ROMERO, Juan Fernando; Calle 56A #81-33, Medellin, Antioquia (CO). CHACON, Camilo; Carrera 79 #34-26, Medellin, Antioquia (CO). JARAMILLO, Gladis; Calle 18 #33-09, El Carmen de Viboral, Antioquia (CO). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW,KZ, LA, LC, LK, LR, LS, LU, LY,MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: MITICIDAL, INSECTICIDAL AND NEMATOCIDAL COMPOSITIONS CONTAINING PLANTS EXTRACTS FROM BULNESIA SP AND HUMULUS SP 2 (57) Abstract: The present disclosure relates to biopesticide compositions comprising aBulnesia sp. plant extract (e.g., an extract from —- Bulnesia sarmientoi) and a Humulus sp. plant extract (e.g., an extract from Humulus lupulus). In some aspects, the Bulnesia sp. plant 25 extract and the Humulus sp. plant extract act synergistically as miticides. Also provided are methods of manufacture, formulations, and kits comprising the disclosed compositions. The disclosed compositions can be use control, treat, or prevent pest infestations (e.g., by mites, insects, nematodes, etc.) in plants and in animals, including humans, in need thereof. MITICIDAL, INSECTICIDAL AND NEMATOCIDAL COMPOSITIONS CONTAINING PLANTS EXTRACTS FROM BULNESIA SP AND HUMULUS SP BACKGROUND [0001] Mites are among the most diverse and successful of all the invertebrate groups. Many species live as parasites on plants and animals. It is estimated that 48,200 species of mites have been described. Many mites which have been well studied are parasitic on plants and animals. Dust mites feed mostly on dead skin and hair shed from humans. Insects may also be infested by parasitic mites. For example, Varroadestructor, attaches to the body of the honey bee, and Acarapis woodi (family Tarsonemidae) lives in the tracheae of honey bees. [0002] Some of the plant pests include the so-called spider mites (family Tetranychidae), thread-footed mites (family Tarsonemidae), and the gall mites (family Eriophyidae). Among the species that attack animals are members of the sarcoptic mange mites (family Sarcoptidae), which burrow under the skin. Demodex mites (family Demodicidae) are parasites that live in or near the hair follicles of mammals, including humans. [0003] Some species of mites can colonize humans directly, act as vectors for disease transmission, or cause or contribute to allergenic diseases. Mites which colonize human skin are the cause of several types of skin itchy rashes, such as grain itch, grocer's itch, and scabies. [0004] Dust mites cause several forms of allergic diseases, including hay fever, asthma and eczema, and are known to aggravate atopic dermatitis. House dust mites are usually found in warm and humid locations, including beds. It is thought that inhalation of mites during sleep exposes the human body to some antigens that eventually induce hypersensitivity reaction. [0005] Many chemicals which possess insecticidal properties have been found to possess little or no effect in killing mites, whereas other chemicals are toxic to mites in the adult stage but not in the egg stage and hence must be applied at very short intervals in order to control the mites from cycle to cycle. Accordingly, there is a need to provide effective miticide compositions that will control mites from the egg to the adult stage. [0006] Frequent treatment of plants with many chemicals which are effective as miticides results in most instances in serious damage to the plant foliage. Most of the chemicals which have been found effective for the control of mites in plants, shrubs and trees, tend to burn the plant. In many instances substantial injury to the foliage is not tolerable. For example, in the control of mites on ornamental plants, only minor degrees of leaf burn can be tolerated. In other instances, for example in the case of citrus fruits, the injury to the fruit caused by the treating chemical frequently gives rise to a down-grading of the fruit that is scarcely less serious to the fruit grower than the injury caused by the presence of mites on the fruit. [0007] The use of chemicals in miticides is also limited because of their toxicity to humans, pets, farm animals, non-target vertebrate and invertebrate species, and the environment. Thus, there is a need for miticides effective on plants, shrubs and trees with negligible injury to the foliage and fruit, and with no toxicity to humans, pets, farm animals, non-target vertebrate and invertebrate species, and the environment. BRIEF SUMMARY OF THE DISCLOSURE [0008] The present disclosure provides compositions comprising Bulnesia sp. plant extracts and Humulus sp. plant extracts that can be used as biopesticides, for example, against acari (mite, ticks), nematodes, insects, etc. In some aspects, the compositions disclosed herein comprise a Bulnesia sp. extract and a Humulus sp. plant extract, wherein the Bulnesia sp. extract and the Humulus sp. plant extract act synergistically as miticides. [0009] In some aspects, the Bulnesia sp. plant is selected from the group consisting of Bulnesia arborea, Bulnesia bonariensis, Bulnesia carrapo, Bulnesia chilensis, Bulnesia foliosa, Bulnesia loraniensis, Bulnesia macrocarpa, Bulnesia rivas-martinezii, Bulnesia retama, Bulnesia sarmientoi, Bulnesia schickendantzii, and a combination thereof. In some aspects, the Bulnesia sp. plant is Bulnesia sarmientoi. In some aspects, the Humulus sp. plant is selected from the group consisting of Humulus japonicus, Humulus lupulus, Humulus yunnanensis, and a combination thereof. In some aspects, the Humulus sp. plant is Humulus lupulus. [0010] In some aspects, the Humulus sp. plant extract is obtained from Humulus sp. leaves. In some aspects, the Humulus sp. leaves are fresh leaves or dry leaves. In some aspects, the fresh leaves or dry leaves are, e.g., shredded, fragmented, pulverized, or pelletized. [0011] In some aspects, the Humulus sp. plant extract is obtained from Humulus sp. flowers or buds (hop cones) or other plant parts (e.g., roots, stems, bark, etc.). In some aspects, the Humulus sp. plant extract is a supercritical C0 2 extract. In some aspects, the Humulus sp. plant extract is obtained by solvent extraction (e.g., using water, organic solvents, or any combination thereof), soxhlet, ultrasonic extraction, or any other method known in the art. [0012] In some aspects, the Humulus sp. plant extract consists or consists essentially of a blend of hop acids with a high concentration of iso alpha acids. Such blend of hop acids in in some aspects a plant extract that has been enriched in iso alpha acids (e.g., by conversion of alpha acids to iso alpha acids, removal on non-iso alpha acid components, addition of iso alpha acids, or any combination thereof). In some aspects, the concentration of iso alpha acids is at least 75%. In some aspects, the concentration of iso alpha acids is at least 90%. In some aspects, the iso alpha acids are derived from humulone, adhumulone, cohumulone, posthumulone, prehumulone, or any combination thereof. [0013] In some aspects, the Bulnesia sp. plant extract is obtained from Bulnesia sp. wood, root, stems, leaves, and other plant parts. In a specific aspect, the Bulnesia sp. plant extract is obtained from Bulnesia sp. wood. In some aspects, the Bulnesia sp. plant extract is obtained by steam flow extraction. In some aspects, the Bulnesia sp. plant extract is obtained by solvent extraction (e.g., using water, organic solvents, or any combination thereof), soxhlet, ultrasonic extraction, or any other method known in the art. In some aspects, the Bulnesia sp. plant extract is an essential oil. [0014] In some aspects, the Bulnesia sp. plant extract comprises guaiol, bulnesol, hanamyol, or any combination thereof. In some aspects, the Bulnesia sp.