Cefas contract report <C5936> TECHNICAL REPORT FOR THE UK NATIONAL REFERENCE LABORATORY FOR ANISAKIS (APRIL 2015 – March 2016) (C5936 - Anisakis NRL) Author: Tom Hill Issue date: 30/3/2016 Cefas Document Control TECHNICAL REPORT FOR THE UK NATIONAL REFERENCE LABORATORY FOR ANISAKIS (APRIL 2015 – MARCH 2016) Submitted to: Chelvi Leonard Date submitted: 30/3/2016 Project Manager: Stephen Feist Report compiled by: Tom Hill Quality control by: Stephen Feist Approved by & Stephen Feist date: 30/3/2016 Version: 1.4 (FINAL) Version Control History Author Date Comment Version Tom Hill 21/03/2016 1st draft 1.0 Tom Hill 22/03/2016 1.1 Tom Hill 23/03/2016 Sent to SWF for comment 1.2 Stephen Feist 23/03/2016 Amendments made to v1.2 1.3 Tom Hill 30/3/2016 PT results added. Minor changes 1.4 to document Tom Hill 30/3/2016 Sent to SWF for approval FINAL Tom Hill 25/4/2016 Report amended with acronym FINAL definitions TECHNICAL REPORT FOR THE UK NATIONAL REFERENCE LABORATORY FOR ANISAKIS Page i TECHNICAL REPORT FOR THE UK NATIONAL REFERENCE LABORATORY FOR ANISAKIS (APRIL 2015 – MARCH 2016) (C5936 – Anisakis NRL) Author: Tom Hill Issue date: 30/3/2016 Head office Centre for Environment, Fisheries & Aquaculture Science Pakefield Road, Lowestoft, Suffolk NR33 0HT, UK Tel +44 (0) 1502 56 2244 Fax +44 (0) 1502 51 3865 www.cefas.defra.gov.uk Cefas is an executive agency of Defra Page ii Page iii Table of contents 1 Introduction ................................................................................................................................... 1 2 Updates to website ....................................................................................................................... 1 2.1 Overview ................................................................................................................................. 1 2.1.1 Website structure ........................................................................................................... 2 2.1.2 Email enquiries ................................................................................................................ 2 3 Participation in European Union Reference Laboratory for Parasites (EURLP) proficiency test scheme (2015 and 2016)................................................................................................................ 3 3.1 Completion of 2015 EURLP proficiency test ........................................................................... 3 3.2 Completion of 2016 EURLP proficiency test ........................................................................... 3 4 Reference material ........................................................................................................................ 4 4.1 Maintenance of tissue bank .................................................................................................... 4 5 Report of the molecular analysis of Anisakis from six species of fish ................................... 5 5.1 Methods .................................................................................................................................. 5 5.2 Results: .................................................................................................................................... 8 6 Attendance at the EURLP annual meeting ................................................................................. 9 6.1 Participation at EURLP annual meeting .................................................................................. 9 6.2 Implementation of EURLP recommendations ........................................................................ 9 7 International Council for the Exploration of the Seas (ICES) Working Group on Pathology and Diseases in Marine Organisms .................................................................................................... 9 8 Ongoing surveillance .................................................................................................................. 11 9 ISO9001:2008 Audit ..................................................................................................................... 12 9.1 Overview ............................................................................................................................... 12 9.1.1 Outcome of audit .......................................................................................................... 12 10 2015/2016 Work plan progress .............................................................................................. 14 10.1 Overview ............................................................................................................................... 14 10.1.1 Anisakis NRL Work plan - progress 2015/16 ................................................................. 14 11 Annex ....................................................................................................................................... 17 11.1 EURLP Proficiency Testing Report 2016 ................................................................................ 17 TECHNICAL REPORT FOR THE UK NATIONAL REFERENCE LABORATORY FOR ANISAKIS Page iv 11.2 ICES WGPDMO meeting. ICES Working Group on Pathology and Diseases in Marine Organisms (WGPDMO). Virginia Institute of Marine Science, 16–20 February 2016. ..................... 18 Page v 1 Introduction The Centre for Environment, Fisheries and Aquaculture Science (Cefas) Weymouth is designated as the UK National Reference Laboratory (NRL) for Anisakis. This annual technical report summarises the activities carried out by the NRL between April 2015 and March 2016 according to the requirements of Regulation (EC) No. 882/2004 and as defined in the Service Level Agreement between the Food Standards Agency and Cefas. 2 Updates to website 2.1 Overview The website continues to deliver information on the responsibilities and function of the NRL. During the period covered by this report, the website was migrated to a new server, and has been updated with quality controlled standard operation procedure documents. Further scientific references and other information resources have also been added. A website analytical report was run 21/03/2016. This determined that the NRL website has had 720 visits to date Figure 1 - Website traffic (page views) 1/1/2014 to 21/3/2016Figure 1) from approx. 362 unique users. The sections of the website which experienced the most traffic were the methodology, proficiency testing, references, and contact sections. Although there is only a small level of traffic moving through the website at this time, the report suggests that interested parties are actively searching for advice and techniques related to Anisakis. Figure 1 - Website traffic (page views) 1/1/2014 to 21/3/2016 2.1.1 Website structure Although content and formatting updates have occurred, the website structure has not changed since the previous reporting period (2015). NRL Home o Background o Regulations . Links to regulations relating to Anisakis o Methods . List of methods from EURL o Proficiency testing o References . List of references o Contact NRL 2.1.2 Email enquiries We have received one email enquiry through the Anisakis mailbox during 2015/2016. This related to an enquiry from the Food Safety and Veterinary Institute in Albania regarding proficiency testing for Anisakis. Advice was provided in a timely manner and contact details were provided for the EURLP who organises such proficiency testing. 3 Participation in European Union Reference Laboratory for Parasites (EURLP) proficiency test scheme (2015 and 2016) 3.1 Completion of 2015 EURLP proficiency test Due to a breakdown in communication with the Union Reference Laboratory for Parasites (EURLP), we were not informed of the 2015 Proficiency Test until after the samples had been sent to participating laboratories. However, following contact with the EURLP director the error was acknowledged and we were included on their ‘Corrective Action’ proficiency test normally offered to those laboratories which had failed the first round. Fillet samples were received on the 15th April 2015 and screened using compression and the ultraviolet transillumination system following internal SOP NRL001. Our results were reported to the EURLP shortly afterwards. We are pleased to report successful completion of the exercise with a score of 100%. 3.2 Completion of 2016 EURLP proficiency test Samples for the 2016 Proficiency Test were received 14th March 2016 and screened using the ultraviolet transillumination system following internal SOP NRL001. Our results were returned to the EURLP 17th March 2016. We received our PT report from the EURLP 29th March and are delighted to confirm successful completion of the exercise with a score of 100% (see annex). 4 Reference material 4.1 Maintenance of tissue bank We are maintaining the Anisakis ‘tissue bank’ on-site. This contains a substantial number of frozen parasites removed from Plaice, Haddock and Mackerel sampled at two recognised sites (St. Bees and Celtic Deep) within the geographical region of the Irish Sea. Although not identified to their species at this time, these samples are available for use in gross (fillet) screening proficiency testing or training programmes. In addition to this material, we have screened a small number of samples of a deep-sea fish species – Aphanopus carbo – caught during a research cruise. Although the material has been held in long term frozen storage a very large number of well-preserved Anisakidae worms were found to be present