Supporting core service facilities for biotechnology research by faculty, student, government, and industry scientists. More Office of Biotechnology at www.biotech.iastate.edu/service_facilities. Protein Facility Iowa State University’s Protein purification of proteins and of protein samples from 1D or 2D Facility provides expertise for the peptides can be accomplished. gels. Gel spots can be digested analysis, characterization, and with a variety of enzymes, and synthesis of proteins and peptides. Mass Spectrometry the resulting peptides can be After training, users can operate A matrix-assisted laser desorption/ analyzed to identify the protein. many instruments themselves. ionization time-of-flight (MALDI- TOF) mass spectrometer can be used SDS-PAGE / Electroblotting for determining the molecular weight The facility conducts sodium of proteins, peptides, glycoproteins, dodecyl sulfate polyacrylamide oligosaccharides, oligonucleotides, gel electrophoresis (SDS-PAGE) of and polymers. A quadrapole- proteins for purity and molecular TOF tandem mass spectrometer weight estimation. Gels can be is also available for obtaining electroblotted to nitrocellulose peptide sequence information. or to PVDF for immuno- detection and protein/peptide Peptide Synthesis sequencing, respectively. The facility can do both large- and small-scale peptide synthesis, 2-D Gel Electrophoresis including phosphopeptides, peptides The facility does two-dimensional with unusual amino acids, and electrophoresis by separating proteins multiple antigenic peptides (MAP). in the first dimension according to charge (isoelectric focusing), Circular Dichroism Protein and Peptide Sequencing followed by separating the focused Researchers who want to detect The Protein Facility provides proteins in the second dimension and quantitate the chirality of N-terminal protein/peptide sequence according to their molecular weight. molecular structures can access the analysis (Edman degradation) Protein Facility’s circular dichroism of proteins in solution or Other Services (CD) spectroscopy services. electroblotted onto polyvinylidene The Protein Facility also has difluoride (PVDF) membrane. equipment for phosphor imaging HPLC and the imaging of visible and Microanalytical, analytical, and In-gel Digestion and Peptide fluorescent stained gels or blots. preparative high performance Mass Fingerprinting Software for the analysis of 1D liquid chromatography (HPLC) The facility offers in-gel digestion and 2D gels is also available. Protein Facility Joel Nott, Manager Hours 7:00 a.m. to 5:00 p.m. weekdays Iowa State University [email protected] Web www.protein.iastate.edu 1178 Molecular Biology Building 515 294-3267 ph Ames IA 50011-3260 Iowa State University does not discriminate on the basis of race, color, age, ethnicity, religion, national origin, pregnancy, sexual orientation, gender identity, genetic information, sex, marital status, disability, or Iowastatus State as a University U.S. veteran. does Inquiries not discriminate can be directed on the tobasis the ofOffice race, of color, Equal age, Opportunity ethnicity, andreligion, Compliance, national 3280origin, Beardshear pregnancy, Hall, sexual orientation, gender identity, genetic information, sex, marital status, disability, or(515) status 294-7612. as a U.S. veteran. Inquiries can be directed to the Office of Equal Opportunity, 3350 Beardshear Hall, (515) 294-7612..