IDENTIFICATION OF SALMONID FISHES FROM TRIBUTARY STREAMS AND LAKES OF THE MID-COLUMBIA BASIN FINAL REPORT - 1998 BY D.S. PROEBSTEL1 R.J. BEHNKE1 S.M. NOBLE2 ________________________________________ 1 World Salmonid Research Institute and Department of Fishery and Wildlife Biology- Colorado State University, Fort Collins, Colorado 80523 2 U.S. Fish and Wildlife Service, Leavenworth, Washington 98826 TABLE OF CONTENTS Page EXECUTIVE SUMMARY .................................................................................................... i BACKGROUND .....................................................................................................................1 Bull Trout ..........................................................................2 Westslope Cutthroat Trout ................................................................3 Interior Redband Rainbow Trout .....................................................5 Taxonomic Concerns ..................................................................................................6 METHODS ..............................................................................................................................7 Collection of Specimens and Preservation of Tissue ...............................................7 Classical Taxonomic Data- Basis of Diagnosis .............................................7 Cutthroat and Rainbow Trout ......................................................................7 Cutthroat Trout ..................................................................................8 Rainbow Trout ....................................................................................9 Bull Trout ........................................................................................................9 Osteological Studies ..........................................................................10 Molecular Genetic Data ...........................................................................................10 DNA Isolation and Analysis .........................................................................10 Polymerase Chain Reaction .........................................................................11 Mitochondrial DNA ..........................................................................11 Restriction Enzyme Digestion ..................................................................11 RESULTS ..............................................................................................................................12 Westslope Cutthroat and Redband Rainbow Trout ..............................................12 Bull Trout ..................................................................................................................13 Mitochondrial DNA Analysis.......................................................................14 Taxonomy ......................................................................................................14 Morphological and Meristic Characters ........................................14 Osteological Characters ...................................................................15 Premaxilla, Supraethmoid, Vomer, Hyomandibulae ....................15 Gill Raker Morphology ....................................................................16 Analysis of Specimens by Subbasin ................................................16 Methow River Subbasin ...........................................................................................16 Lower Methow River (Mouth to Winthrop) ..........................................................16 Black Canyon Creek .....................................................................................16 Methow River, mainstem .............................................................................16 Gold Creek ....................................................................................................17 South Fork Gold Creek ....................................................................17 Foggy Dew Creek ..............................................................................17 Crater Creek .....................................................................................18 Libby Creek ...................................................................................................19 North Fork Libby Creek ..................................................................19 Beaver Creek .................................................................................................20 Twisp River Watershed ................................................................................20 Twisp River, mainstem .....................................................................20 Little Bridge Creek ...........................................................................22 Buttermilk Creek ..............................................................................22 West Fork Buttermilk Creek ...............................................22 East Fork Buttermilk Creek ................................................23 Eagle Creek .......................................................................................23 Middle Oval Lake .................................................................24 West Oval Lake .....................................................................25 War Creek .........................................................................................25 Reynolds Creek .................................................................................26 South Creek .......................................................................................26 North Creek .......................................................................................27 Upper Methow River (Winthrop to Headwaters) ..................................................27 Methow River, mainstem .............................................................................27 Chewuch River Watershed ..........................................................................28 Chewuch River, mainstem ...............................................................29 Cub Creek ..........................................................................................30 Boulder Creek ...................................................................................30 Eight Mile Creek ...............................................................................31 Falls Creek .........................................................................................31 Twentymile Creek .............................................................................32 Lake Creek ........................................................................................32 Andrews Creek ..................................................................................33 Tungsten Lake ...................................................................................34 Wolf Creek ....................................................................................................34 Goat Creek ....................................................................................................35 Early Winters Creek ....................................................................................36 Cedar Creek ......................................................................................37 Cutthroat Creek ................................................................................38 Lost River ......................................................................................................38 Cougar Lake ......................................................................................39 Monument Creek ..............................................................................39 West Fork of the Methow River ..................................................................39 Robinson Creek .................................................................................39 Trout Creek ....................................................................................40 Entiat River Subbasin ..............................................................................................40 Entiat River, mainstem ................................................................................40 Roaring Creek ...............................................................................................41 Mad River Watershed ..................................................................................41 Mad River, mainstem .......................................................................41 Tillicum Creek ..................................................................................42 Hornet Creek .....................................................................................43 Cougar Creek ....................................................................................43 Miners Creek .....................................................................................44 Preston Creek ................................................................................................44 Tommy Creek................................................................................................44 Lake Creek ....................................................................................................44 North Fork Entiat River ..............................................................................45 South Pyramid Creek .......................................................................45