CDH10 Antibody Cat. No.: 58-022 CDH10 Antibody Flow cytometric analysis of CEM cells using Cadherin 10 Formalin-fixed and paraffin-embedded human brain (CDH10) Antibody (bottom histogram) compared to a tissue reacted with CDH10 antibody (N-term), which was negative control cell (top histogram). FITC-conjugated peroxidase-conjugated to the secondary antibody, goat-anti-rabbit secondary antibodies were used for the followed by DAB staining. analysis. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human This CDH10 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 25-55 amino acids from the N-terminal region of human CDH10. TESTED APPLICATIONS: Flow, IHC-P, WB September 23, 2021 1 https://www.prosci-inc.com/cdh10-antibody-58-022.html For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 PREDICTED MOLECULAR 88 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: CDH10 ALTERNATE NAMES: Cadherin-10, T2-cadherin, CDH10 ACCESSION NO.: Q9Y6N8 PROTEIN GI NO.: 116241276 GENE ID: 1008 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 23, 2021 2 https://www.prosci-inc.com/cdh10-antibody-58-022.html CDH10 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular BACKGROUND: domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. REFERENCES: 1) Kools,P., FEBS Lett. 452 (3), 328-334 (1999) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 23, 2021 3 https://www.prosci-inc.com/cdh10-antibody-58-022.html.