antibodies for developmental biology ptglab.com antibodies people trust Antibodies for Developmental biology 1 WELCOME... FOREWORD Developmental biology covers a broad spectrum of scientific research relating to the growth and development of living things. Not only does it concern the embryogenic events immediately following fertilization, it also encompasses the genetic control of cell growth, differentiation and morphogenesis – key components of regeneration and aging in the adult organism. In this development-focused catalog you will find This catalog is essentially a shortlist of around one quarter You can find us at antibodies to those targets involved in pattern formation, of the antibodies Proteintech has for developmental www.ptglab.com such as HOX gene products and proteins involved in Notch biology protein targets. If you can’t find an antibody to and Hedgehog signaling; neural tube formation, such as your target of choice here, we’re confident that with over sonic hedgehog protein; and organogenesis such as Wnt, 2,000 primary antibodies relating to development in the FGF, BMP and EYA proteins. At the center of the catalog, complete Proteintech inventory you’ll find what you’re you will also find a primer on the primary cilium, with looking for online at www.ptglab.com. details on Proteintech antibodies recognizing the proteins involved in the generation and maintenance of this vital developmental structure. ABOUT US Proteintech was established in 2002 by a research individual gene targets, all made at its own laboratory collective based at the University of Illinois at Chicago. facilities. With one hundred percent original products, They set out to provide fellow researchers with antibodies Proteintech offers its customers sincerity, integrity and made to the highest standards straight from their own originality. You can only buy Proteintech antibodies directly laboratory, and would never source a single antibody from from Proteintech or via one of its approved distributors — any other provider. when you receive your antibody and see the Proteintech logo on the vial, you hold a truly unique research reagent. Since the first few hundred antibodies Proteintech has grown to offer thousands of antibodies against as many WHAT’S INSIDE As an original manufacturer Proteintech can offer you: 2-3 Antibodies for Cilia Development o All of its production data available freely or on request. 4-5 Antibodies: ABLIM1 DHH o 100% original products; no third-party suppliers, 6-7 Antibodies: DISC1-long-specific HOMEZ no duplicates. 8-9 Cilia Pathway o Bulk purchasing options. o Bespoke buffer compositions on request. 10-11 Antibodies: HOPX PCDHA5 o A fully comprehensive guarantee. 12-13 Antibodies: PCDHA6 VEGFR3 14 Antibodies: Vimentin ZHX2 15 Focus Article: Six2 and Kidney Development 16 Contact 2 ptglab.com ANTIBODIES FOR CILIA DEVELOPMENT by Deborah Grainger The primary cilium acts as a sensory organelle that transfers information from the environment to the cell interior. Once thought of as an evolutionary artifact, this organelle is now understood to be crucial for regulating important cellular processes, including the cell cycle, cytoskeletal organization, intraflagellar transport and signaling pathways such as hedgehog, notch and canonical and non-canonical Wnt/planar call polarity (PCP) pathways. Proteintech has over 70 antibodies recognizing cilia-related proteins in its catalog. This article reviews a selection of these targeting proteins involved in cilia development, or ciliogenesis… IFT88 IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of Acetylate Tubulin IFT88 Merged IFT particles required for cilium biogenesis. Along with other molecular motors and IFT particles, IFT88 helps mediate intraflagellar transport, an important process essential for the assembly and maintenance of both primary and motile cilia and flagella in many organisms. IFT88 also localizes to spindle poles during mitosis and is required for spindle orientation. Please contact us Defects in IFT88 lead to polycystic kidney with any technical disease, characterized by progressive cyst Immunofluorescence staining of primary cilia using anti-IFT88 (13967-1-AP), development and bilaterally enlarged kidneys. counterstained with an anti-acetylated tubulin antibody. Figure provided by questions you Dr. Kevin Corbit and Dr Andrew Kodani of the Reiter Lab at UCSF. may have. Proteintech’s polyclonal IFT88 antibody (13967-1-AP) has appeared in over 40 publications to date, recently appearing in Nature Cell the original shape of the cilium, while acetylated tubulin Biology in June 2014. The paper looks at the role of another marks the shrinking microtubules of the retreating cilium. protein, Kif7, in mammalian Hedgehog (Hh) signaling and cilium tip organization, but utilizes anti-IFT88 as a marker The Proteintech polyclonal IFT88 antibody was raised for cilium retraction along with acetylated tubulin in against the C-terminal region of human IFT88 and can detect immunofluorescence staining. The IFT88 signal outlines endogenous levels of IFT88. CP110 CP110, also named CCP110 or KIAA0419, is a 110 kDa utilized for Western blotting (WB) and immunofluorescence centriolar protein, not to be confused with CEP110 studies looking at the impact of blocking or upregulating (centriolin). CP110 positively regulates centriole duplication M129 on ciliogenesis and cilia elongation. Cilia formation while restricting centriole elongation and ciliogenesis.It acts was inhibited when M129 levels were depleted, as a key negative regulator of ciliogenesis in collaboration and, conversely, potently upregulated with M129 with CEP97 by capping the mother centriole, thereby overexpression. Correspondingly, WB experiments showed preventing cilia formation. that CP110 protein levels were depleted in the presence of overexpressed M129. Several other Proteintech antibodies The Proteintech antibody anti-CP110 (12780-1-AP) has were also used by the authors for this work, including those appeared in several publications since its first appearance targeting housekeeping protein GAPDH (60004-1-Ig), the in Nature Cell Biology in mid-2012. In this paper the authors actin nucleation protein ARP2 (10922-1-AP) and actin describe CP110’s regulation by the microRNA miR-129- binding LIM protein 1 (ABLIM1, 15129-1-AP). 3p (M129). The Proteintech anti-CP110 antibody was Antibodies for Developmental biology 3 ARL13B ARL13B, (also named ARL2L1), is a small ciliary G protein including one that further investigates its interaction with of the Ras superfamily. Localized to cilia, it is required the phosphatase inositol polyphosphate-5-phosphatase for cilium biogenesis as well as sonic hedgehog signaling E (INPP5E) – another JBTS-causing protein when mutated. and antibodies targeting ARL13B can be used to mark Along with a C-terminal motif and prenylation signals, the cilium (PMID:22072986). Defects in ARL13B lead ciliary targeting of INPP5E is facilitated by ARL13B. to Joubert syndrome (JBTS), an autosomal recessive ARL13B missense mutations that cause JBTS in humans disorder characterized by malformation of the cerebellum. disrupt the ARL13B–INPP5E interaction. The paper, Consequentially JBTS patients lack muscle control and which appeared in PNAS at the end of 2012, identifies tone, among other defects. several more centrosomal and ciliary proteins involved in ARL13B–INPP5E interactions, and found that this functional Proteintech’s anti-ARL13B antibody (17711-1-AP) has network of proteins is also involved in JBTS and the related featured in 30 peer-reviewed publications to date, ciliopathy nephronophthisis. Acetylated α-tubulin (K40) The acetylation of K40 on α-tubulin is a hallmark of stable microtubules, and anti-acetylated α-tubulin (anti-ace tubulin) is the antibody of choice for researchers wishing to perform control immunofluorescence staining of stable cilia. The acetylated residue of α-tubulin is K40, which is catalyzed by α-tubulin acetyl-transferase (α-TAT). Proteintech’s anti-ace tubulin (K40) antibody (66200-1- Ig) can be used to reliably mark the cilium; as mentioned Immunofluorescence analysis of MDCK cells using TUBA1A previously, it can also be used to gauge cilium retraction in (ace-Lys40 antibody (66200-1-Ig; 1:50) and Alexa Fluor tandem with IFT88. 488-congugated AffiniPure Goat Anti-Mouse IgG. BBS2 No information is available regarding the specific function core of the BBSome. The BBSome is a component of the of Bardet–Biedl syndrome (BBS) protein 2, but it is known to ciliary basal body and is integral to the formation of a be one of the seven BBS proteins that form the stable functional primary cilium as mutations in BBS proteins, as well as several others, lead to their eponymous syndrome BBS. BBS is a heterogeneous, pleiotropic human disorder characterized by obesity, retinopathy, polydactyly, renal and cardiac malformations, learning disabilities, hypogenitalism, and an increased incidence of diabetes and hypertension. Proteintech’s BBS2 antibody (11188-2-AP) has appeared in several publications since its addition to the catalog. One of these appearances, in the Journal Cell, describes how the BBSome establishes an electron-rich coat complex that You can find us at sorts membrane proteins to primary cilia, including the www.ptglab.com somatostatin receptor 3 (SSTR3) signaling molecule. The BBSome coat model suggested by the authors could explain the variety of symptoms found in BBS patients: likely resulting from the failure to transport signaling