Product datasheet Anti-MED18 Antibody Catalog Number: A10600-2 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-MED18 Antibody Gene Name MED18 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,FCM,Direct ELISA,ICC/IF Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E. coli-derived human MED18 recombinant protein (Position: M1-M208). Purification Immunogen affinity purified. Observed MW 24KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section: 1:50-400 Flow Cytometry: 1-3μg/1x106 cells Direct ELISA： 1:100-1000 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 directly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence. Reference Anti-MED18 Antibody被引用在0文献中。 暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-MED18 Antibody Catalog Number: A10600-2 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot Figure 2. IHC analysis of analysis of MED18 using MED18 using anti-MED18 anti-MED18 antibody antibody (A10600-2). Lane 1: (A10600-2).MED18 was human A431 whole cell detected in paraffin- lysates, Lane 2: human embedded section of K562 whole cell human intestinal cancer lysates,Lane 3: human tissue. Biotinylated goat U2OS whole cell anti-rabbit IgG was used lysates,Lane 4: human as secondary antibody . HL-60 whole cell The tissue section was lysates,Lane 5: rat liver developed using Strepavi tissue lysates,Lane 6: din-Biotin-Complex mouse kidney tissue (SABC)(Catalog # lysates. anti-MED18 SA1022) with DAB as the antigen affinity purified chromogen. polyclonal antibody (Catalog # A10600-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for MED18 at approximately 24KD. The expected band size for MED18 is at 24KD. Figure 3. IHC analysis of Figure 4. IHC analysis of MED18 using anti-MED18 MED18 using anti-MED18 antibody antibody (A10600-2).MED18 was (A10600-2).MED18 was detected in paraffin- detected in paraffin- embedded section of embedded section of human lung cancer mouse brain tissue. tissue. Biotinylated goat Biotinylated goat anti- anti-rabbit IgG was used rabbit IgG was used as as secondary antibody . secondary antibody . The The tissue section was tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC)(Catalog # (SABC)(Catalog # SA1022) with DAB as the SA1022) with DAB as the chromogen. chromogen. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-MED18 Antibody Catalog Number: A10600-2 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3 Powered by TCPDF (www.tcpdf.org).