GoldBio © 2015 All Rights Reserved This Publication is a creation of Gold Biotechnology and is intended as a sourcebook for research laboratories. This publication may not be redistributed or copied for commercial use. Procedure for use with Gold Biotechnology Buffers; Table of Contents Introduction .................................................................................................................................................. 5 What is a buffer and how do they work? ................................................................................................. 5 Why is this important for biologists? ........................................................................................................ 6 Why are there so many different buffers and how do I decide which one to use? ............................... 6 What are Good’s Buffers? ..................................................................................................................... 6 Table 1. List of Good’s Buffers offered by GoldBio in order of pKa. ..................................................... 8 How to Choose the Right GoldBio Buffer for Your Experiment .................................................................... 9 How to Make Your Buffer Solution ............................................................................................................. 10 Temperature Effects ............................................................................................................................... 11 GoldBio Buffers Grouped By Structural Family ........................................................................................... 12 Acetamido Family (ACES, ADA) ............................................................................................................... 12 ACES .................................................................................................................................................... 13 ADA ..................................................................................................................................................... 14 Bis(2-hydroxyethyl)amine Family (Bis-Tris, BES, Bicine) ......................................................................... 15 Bis-Tris ................................................................................................................................................. 15 BES....................................................................................................................................................... 16 Bicine ................................................................................................................................................... 17 Cyclohexylamino Family (CHES, CAPS) .................................................................................................... 17 CHES .................................................................................................................................................... 18 CAPS .................................................................................................................................................... 19 Morpholinic Family (MES, MOPS, MOPSO) ............................................................................................ 19 MES ..................................................................................................................................................... 20 MOPS................................................................................................................................................... 21 MOPSO ................................................................................................................................................ 22 Piperazinic Family (PIPES, HEPES, HEPPSO) ............................................................................................ 22 PIPES .................................................................................................................................................... 23 Gold Biotechnology St. Louis, MO Ph : (800)248-7609 Web: www.goldbio.com 2 Email: [email protected] HEPES .................................................................................................................................................. 24 HEPPSO ............................................................................................................................................... 26 Tris Family (Tris, Bis-Tris, TES, TAPS, Tricine) .......................................................................................... 26 Bis-Tris ................................................................................................................................................. 26 TES ....................................................................................................................................................... 27 Tricine .................................................................................................................................................. 27 Tris ....................................................................................................................................................... 29 TAPS .................................................................................................................................................... 30 Other buffers........................................................................................................................................... 30 PBS (Phosphate Buffered Saline) ........................................................................................................ 30 Boric Acid ............................................................................................................................................ 31 FAQS ............................................................................................................................................................ 32 Troubleshooting .......................................................................................................................................... 33 Protocols ..................................................................................................................................................... 34 0.1M ACES Buffer - 1 L ............................................................................................................................ 35 0.5M ADA Buffer - 1 L ............................................................................................................................. 36 1M BES Buffer - 1 L .................................................................................................................................. 37 1M Bicine Buffer - 1 L .............................................................................................................................. 38 1M Bis-Tris Buffer - 1 L ............................................................................................................................ 39 0.5M CAPS Buffer - 1 L ............................................................................................................................ 40 0.5M CHES Buffer - 1 L ............................................................................................................................ 41 1M HEPES Buffer - 1 L ............................................................................................................................. 42 1M HEPES-Na Buffer - 1 L ........................................................................................................................ 43 1M HEPPSO Buffer - 1 L .......................................................................................................................... 44 0.5M MES Buffer - 1 L ............................................................................................................................. 45 0.5M MES Monohydrate Buffer - 1 L ...................................................................................................... 46 0.5M MES-Na Buffer - 1 L ........................................................................................................................ 47 1M MOPS Buffer - 1 L .............................................................................................................................. 48 1M MOPS-Na Buffer - 1 L ........................................................................................................................ 49 0.5M MOPSO Buffer - 1 L ........................................................................................................................ 50 0.5M MOPSO-Na Buffer - 1 L .................................................................................................................. 51 1M PIPES Buffer - 1 L ............................................................................................................................... 52 1M PIPES-Na Buffer - 1 L ......................................................................................................................... 53 Gold Biotechnology St. Louis, MO Ph : (800)248-7609 Web: www.goldbio.com 3 Email: [email protected] 1M TAPS Buffer - 1 L ............................................................................................................................... 54 1M TES Buffer - 1 L .................................................................................................................................