IMMUNOASSAY WORKFLOW SOLUTIONS ANTIBODY ARRAYS, LUMINEX® ASSAYS, ELISAS & SIMPLE PLEX™ ASSAYS TABLE OF CONTENTS Which Immunoassay is Right For You? ..................................................................................3 What’s New ........................................................................................................................... 4-5 Immunoassay Options from Discovery to Validation ...........................................................6 Move Between Immunoassay Platforms with Confidence .................................................7 Immunoassay Offering by Molecule ...............................................................................8-36 Luminex High Performance Fixed Panels ......................................................................37-38 Luminex High Performance Assays ................................................................................39-40 Proteome Profiler Antibody Arrays ................................................................................41-51 2 WHICH IMMUNOASSAY IS RIGHT FOR YOU? NUMBER OF SAMPLE KIT FORMAT BENEFIT ASSAY TIME INSTRUMENT ANALYTES VOLUME Most Quantikine ELISA 1 96-well plate Published 10-200mL 3-5 Hours Plate Reader Low CVs Simplified protocol Quantikine QuicKit 1 96-well plate 50mL 1.5 Hours Plate Reader Results in 90 mins Quantikine HS Highest 1 96-well plate 10-200mL 4-4.5 Hours Plate Reader ELISA Sensitivity Economical DuoSet ELISAs 1 Flexible Largest Menu 100mL 20 Hours Plate Reader Flexible Hands Free Simple Plex Assays Up to 8 Cartridge 2.5-25mL 1.5 Hours ELLA Sensitive Completely Luminex High Up to 45 Bead-based Optimized 25mL 4 Hours Luminex Performance Assays Panels Luminex Discovery Most Flexible Up to 50 Bead-based 25mL 4 Hours Luminex Assays Panels Largest Proteome Profiler Membrane Imaging Up to 119 Screening 50-500mL 20 Hours Arrays Array System Capabilities Learn more | rndsystems.com/immunoassays 3 WHAT’S NEW? LUMINEX LEARNING LUMINEX ASSAY USERS LUMINEX HIGH ACADEMY GUIDE PERFORMANCE PANELS FOR IMMUNE PROFILING The MagPix® Luminex® Download to Learn: Learning Plan at the • Human XL Cytokine Bio-Techne Academy has • What is a Luminex assay 45-plex Fixed Panel – LKTM014 all the training you need to • Which Luminex assay is right get up and running on your for you • Human XL Cytokine MagPix® Instrument. The Configurable Panel – course includes modules • How to ensure performance FCSTM18 on Luminex technology, and consistency • Human Th1/Th2 installation, ordering and • Multiplex assay best practices 11-plex Fixed Panel – running R&D Systems® and techniques LKTM008 assays, working in xPonent, maintenance protocols • And More! • Human Th9/Th17/Th22 17-plex Fixed Panel – and troubleshooting. LKTM009 So, whether you need a refresher, have a new • Human Immunotherapy colleague that needs 24-plex Fixed Panel – LKTM010 training, or need to get certified to use MagPix, the • NHP XL Cytokine Bio-Techne Academy is the Configurable Panel – place to learn. Self-register FCSTM21 at Academy.Bio-techne.com. 4 PROTEIN A ELISA INTERFERON GAMMA FAST & REPRODUCIBLE COVID-SEROINDEX KIT FOR ANTIBODY ELISAS HOST CELL PROTEIN KANTARO MANUFACTURING DETECTION IN THE QUANTITATIVE SARS- Interferon Gamma BIOPROCESSING COV-2 IGG ANTIBODY (IFN-gamma) is central to During antibody WORKFLOW RUO KIT POWERED BY manufacturing, Protein A many cellular and immune R&D SYSTEMS or its fragments may leach processes. Accurate Chinese Hamster Ovary from a purification column quantification of this cell (CHO) host cell The COVID-SeroIndex and remain bound to the cytokine is crucial in a proteins (HCPs) are a is a quantitative IgG Fc region of a therapeutic variety of workflows source of contamination antibody enzyme-linked antibody. The Protein A ranging from basic during the production of immunosorbent assay ELISA kit is for the detection immunology research to recombinant therapeutic (ELISA) kit that enables an of natural and recombinant cell therapy manufacturing. proteins or biologics. objective measurement of Protein A constructs while Learn more about all of Business and regulatory SARS-CoV-2 IgG antibodies the Protein A Engineered Bio-Techne’s IFN-gamma standards require accurate which is indicative of prior Variant ELISA kit detects immunoassays. quantification of CHO infection. This 2-phase unnatural recombinant HCPs in all in-process ELISA is accurate, proven, • Human IFN-gamma Protein A constructs. samples during the and accessible. Quantikine ELISA Learn more about Protein bioprocess workflow. Learn more about the Kit – DIF50C A ELISAs. Download the application COVID-SeroIndex ELISA. • Human IFN-gamma note to learn how Cygnus • Protein A ELISA • COVID-SeroIndex Quantikine HS ELISA Technologies CHO HCP Kit – BPPA00 ELISA – DSR200 Kit – HSDIF0 3G-1 antibodies pair with • Protein A ELISA Kit for Simple Plex assays on Ella Engineered Variant – for fast and reproducible BPPAEV0 HCP detection. • Learn about Simple Plex Assays and Ella • CHO HCP 3G-1 Simple Plex Assay 5 IMMUNOASSAY OPTIONS FROM DISCOVERY TO VALIDATION MULTIPLEX ASSAYS DISCOVERY VALIDATION Proteome ProfilerTM Luminex® Luminex® High Simple PlexTM Arrays Discovery Assays Performance Assays Multiplex Cartidges • Analyze up to 119 • Customized panels. • Rigorously validated for • Automated detection proteins simultaneously. accuracy, precision, and of up to 8 analytes. • Quantify up to 50 reproducibility. • Quickly assess complex analytes simultaneously. • High throughput. signaling responses. • Flexible panels available. SINGLE ANALYTE ASSAYS DISCOVERY VALIDATION RESULTS Duosets® Development ELISAs Quantikine® ELISAs Simple PlexTM Single or Multianalyte Cartridges • Economical and adaptable to • Exhaustively validated for superior • Hands-Free. Data in 90 minutes. multiple platforms. performance and reproducibility. • Multiple formats available. • Over 1,000 kits available covering • The most referenced ELISAs in 16 species. literature. 6 MOVE BETWEEN IMMUNOASSAY RELIABLE PLATFORMS WITH CONFIDENCE BIO-TECHNE IS YOUR IMMUNOASSAY PARTNER. REPRODUCIBLE POWERED BY R&D SYSTEMS We offer a full catalog of immunoassay solutions so you can find the best platform for your needs. When your needs CONSISTENT MOST PUBLISHED change, you can also move between platforms with confidence CUSTOMIZABLE and consistency in your results. High Performance Luminex QUALITY ROBUST Assays and Simple Plex Cartridges are both correlated to the ACCURATE gold standard Quantikine ELISA. Whether you are starting with a multiplex assay or single analyte, we have your current and future needs covered. A. B. QUALITY AT THE CORE • Core Reagents – R&D Systems antibodies, proteins, and specialized diluents are the foundation of all our immunoassays. High quality raw materials are essential for building accurate and reproducible assays. • Expertise – Bio-Techne brands have over 35 years of C. experience developing and manufacturing immunoassays. Our expertise is used to build the most reliable assays with superior accuracy and precision. • Accuracy – Specialized diluents are used to overcome effects seen by complex sample matrices, so you get the most accurate and trusted results. • Quality Control – Every raw material and complete kit goes through stringent quality control testing to make sure your assays remain consistent over time and your data is reproducible. • Custom Services – Customized immunoassay solutions including modification of an existing assay, species testing Serum IFN-gamma detection is highly correlated across Bio-Techne’s immunoassay and qualification, and new immunoassay development are platforms. A. The Quantikine HS ELISA is highly correlated with Simple Plex Assay (R2=0.997). B. Similarly, HS ELISA data is also highly correlated with Luminex assay available to fit your experimental needs. data (R2=0.97). C. Finally, data collection using the Simple Plex Assay is highly correlated with Luminex Assay (R2=0.98). 7 IMMUNOASSAY OFFERING BY MOLECULE PROTEOME LUMINEX LUMINEX HIGH DUOSET QUANTIKINE SIMPLE MOLECULE PROFILER DISCOVERY PERFORMANCE UNCOATED COATED PLEX ARRAYS ASSAYS ASSAYS ELISAS ELISAS ASSAYS 14-3-3 zeta H 20S Immunoproteasome H 25-OH Vitamin D 4-1BB/TNFRSF9/CD137 H H M ACE/CD143 H H M H Acetylcholinesterase/ACHE H Activin A H H H M R ADAM10 H ADAM12 H H ADAM15 H H ADAM8 H H ADAM9 H H ADAMTS1 H H ADAMTS13 H H H H ADAMTS4 H ADAMTS5 H Adiponectin/Acrp30 H M R H M H H M R H M R Afamin H Aggrecan H H Agrin R AgRP/ART M H H H Akt, Phospho (S473) Pan Specific H H M R Akt1 H M R Akt1, Phospho (S473) H M Akt2 Akt3 Albumin H ALCAM/CD166 H H H M Aldehyde Dehydrogenase 1-A1/ALDH1A1 H Aldosterone MS ALK-1 H alpha 1-Acid Glycoprotein H H alpha 1B-Glycoprotein H alpha 1-Microglobulin H alpha 2-Macroglobulin H H KEY: Human H, Mouse M, Rat R, Canine C, Primate Pr, Porcine P, Multi-Species MS, Viral V, Bovine B, C. botulinum Cb, Cotton Rabbit CR, Equine E, Guinea Pig G, Rabbit Rb, *In Development Learn more | rndsystems.com/immunoassays 8 IMMUNOASSAY OFFERING BY MOLECULE PROTEOME LUMINEX LUMINEX HIGH DUOSET QUANTIKINE SIMPLE MOLECULE PROFILER DISCOVERY PERFORMANCE UNCOATED COATED PLEX ARRAYS ASSAYS ASSAYS ELISAS ELISAS ASSAYS alpha-Defensin 1 H alpha-Fetoprotein/AFP H H H M H M H alpha-N-acetylglucosaminidase/NAGLU H alpha-Synuclein H H AMICA/JAML H Aminoacylase/ACY1 H Aminopeptidase N/CD13 H H Amphiregulin H M H M H H AMPK alpha 1 H H M R AMPK alpha 1, Phospho (T183) H Amyloid beta H Amyloid beta aa 1-42 H Angiogenin H H H H H Angiopoietin-1 H M H H H H Angiopoietin-2 H M H M H H M R H M R Angiopoietin-3