Xylella fastidiosa & the Olive Quick Decline Syndrome (OQDS) A serious worldwide challenge for the safeguard of olive trees Les opinions, les données et les faits exposés dans ce numéro sont sous la responsabilité des auteurs et n'engagent ni le CIHEAM et la FAO, ni les Pays membres. Opinions, data and information presented in this edition are the sole responsibility of the author(s) and neither CIHEAM and FAO nor the Member Countries accept any liability therefor. CIHEAM Xylella fastidiosa & the Olive Quick Decline Syndrome (OQDS) A serious worldwide challenge for the safeguard of olive trees Scientific Editors: A. M. D’Onghia, S. Brunel, F. Valentini Compilation: A. M. D’Onghia and M. Digiaro Language revision: E. Lapedota OPTIONS méditerranéennes Head of Publication: Cosimo Lacirignola 2017 Series A: Mediterranean Seminars Number 121 Centre International de Hautes Etudes Agronomiques Méditerranéennes International Centre for Advanced Mediterranean Agronomic Studies L'édition technique, la maquette et la mise en page de ce numéro d'Options Méditerranéennes ont été réalisées par l'Atelier d'Édition de l'IAM de Bari (CIHEAM) Technical editing, layout and formatting of this edition of Options Méditerranéennes was performed by the Editorial Board of MAI Bari (CIHEAM) The Olive Quick Decline Syndrome (OQDS), photo courtesy of Dr. Franco Valentini, CIHEAM - MAIB The Meadow Froghopper philaenus spumarius, photo courtesy of Dr. Vincenzo Cavalieri, IPSP-CNR, Italy Tirage / Copy number : 100 Ideaprint - Bari, Italy e-mail: [email protected] Comment citer cette publication / How to quote this document : A. M. D’Onghia, S. Brunel, F. Valentini. Xylella fastidiosa & the Olive Quick Decline Syndrome (OQDS) A serious worldwide challenge for the safeguard of olive trees - IAM Bari: CIHEAM (Centre International de Hautes Etudes Agronomiques Méditerranéennes), 2017 – 172 p. (Série A Mediterranean Seminars, N° 121, Options Méditerranéennes) Catalogue des numéros d'Options Méditerranéennes sur / Catalogue of Options Méditerranéennes issues on : www.ciheam.org/publications ISSN : 1016-121X ISBN : 978-2-85352-570-1 © CIHEAM, 2017 Reproduction partielle ou totale interdite sans l'autorisation du CIHEAM Reproduction in whole or in parts is not permitted without the consent of the CIHEAM Contents Foreword ...................................................................................................................................................... 5 Introduction CIHEAM actions to combat X. fastidiosa in the Mediterranean region – Lacirignola C. Raeli M. ..................................................................................................................................... 9 FAO preventive actions to the introduction and spread of the OQDS – Xylella fastidiosa in NENA Region – Al-Dobai S., Nasr N. ...................................................................... 11 The International Plant Protection Convention (IPPC) and its standards on pest surveillance and diagnostics to prevent the entry of Xylella fastidiosa – Gonçalves Moreira A., Xia J., Brunel S. .......................................................................................................... 13 Overview of the activities and projects on Xylella fastidiosa of the International Olive Council (IOC) – Ghedira A., Serafini F. ........................................................................................... 15 From science to policy, the contributions of EPPO and Euphresco to the Xylella fastidiosa emergency – Petter F., Giovani B., Roy A.S. ................................................................ 19 Part One General overview, surveillance & diagnostic methods The world threat of Xylella fastidiosa – López M. M., Marco-Noales E., Peñalver J., Morente C., Monterde A. .............................................................................................................................. 23 The olive quick decline syndrome – Martelli G. P., Nigro F. ................................................................. 25 Xylella fastidiosa and its role in the Olive Quick Decline Syndrome – Saponari M., Boscia D., Martelli G. P.. ............................................................................................................................... 29 Main insect vectors of Xylella fastidiosa in Italy and worldwide – Cavalieri V., Porcelli F. ................................................................................................................................... 31 Xylella fastidiosa: the status of the infection and control measures in France – Legendre B., Denancé N., Olivier V., Molusson D., Juteau V., Agud-Miguel D., Sainte-Luce A., Dousset C., Audusseau C., Paillard S., François C., Rivoal C., Germain J.F., Reynaud P., de Jerphanion P., Joudar S., Francart J., Poirier A., Lecat M., Poliakoff F., Jacques M.A. ............................................................................................................... 33 State of the art of the research on X. fastidiosa in Puglia – Saponari M., .......................................... 37 Current EU research initiatives on Xylella fastidiosa – Boscia D., Saponari M. .................................. 41 CIHEAM/IAMB innovative tools for early surveillance and detection of Xylella fastidiosa – D’Onghia A. M. .......................................................................................................... 43 Options Méditerranéennes, A No.121, 2017 - Xylella fastidiosa & the Olive Quick Decline Syndrome (OQDS) A serious worldwide challenge for the safeguard of olive trees IT platform based on smart device and web-application for the survey of Xylella fastidiosa – Santoro F., Gualano S., Favia G., D’Onghia A. M. ....................................................... 47 Sampling procedures of plant material for the survey of Xylella fastidiosa in Puglia Region, Italy – Valentini F., Cavallo G., D’Onghia A. M. ............................................................. 49 The “Spy Insect” approach for monitoring Xylella fastidiosa in absence of symptomatic plants – Yaseen T., Valentini F., Santoro F., Lorusso D., D’Onghia A. M. ........................... 53 Use of conventional DNA- and protein-based techniques for the detection and characterization of Xylella fastidiosa in Italy – Elbeaino T., Digiaro M., .............................................. 55 PCR assays for the detection of Xylella fastidiosa Review and comparison of published protocols – Reisenzein H. ....................................................... 57 Direct Tissue ImmunoBlot assay (DTBIA), an efficient tool for the mass detection of Xylella fastidiosa in infected olive trees – Djelouah K., Frasheri D. ....................................................... 61 Specific, Sensitive, and Rapid Diagnosis of Xylella fastidiosa from olive plant material by a new Loop-Mediated Isothermal Amplification (LAMP) system – Yaseen T., Si Ammor M., Casini G., Drago S., Stampone G., Elbeaino T., Digiaro M. ....................................... 65 Organization of ring tests on diagnostic methods among Italian laboratories – Loreti S., Pucci N., Loconsole G., Modesti V., Potere O., Lucchesi S., Gaffuri F., Saponari M. ........................... 69 Part Two Control strategies, legislative aspects, capacity development and communication Transcriptome profiling of two olive cultivars infected by Xylella fastidiosa – Giampetruzzi A., Morelli M., Saponari M., Loconsole G., Chiumenti M., Boscia D., Savino V.N., Martelli G.P., Saldarelli P. ............................................................................................................ 73 Preliminary results on field trials to control Xylella fastidiosa on olive trees in Puglia – Scortichini M. ............................................................................................................................................... 77 Preliminary results of comparative efficacy evalutation trials against Philaenus spumarius L., vector of Xylella fastidiosa – Dongiovanni C., Cavalieri V., Altamura G., Di Carolo M., Fumarola G., Saponari M., Porcelli F. ............................................... 79 Sustainable strategies to contain the Olive Quick Decline Syndrome in southeast Italy – Carlucci A., Ingrosso F., Lops F. ...................................................................................................................... 81 Good agricultural practices in the management of the Olive Quick Decline Syndrome – Xiloyannis C., Mininni A.N., Lardo E., Miccoli A., Fausto C. ........................................................................ 83 Risk assessment of Xylella fastidiosa in the EU territory and other EFSA activities – Winter S., Bragard C., Tramontini S., Andueza M., Stancanelli G. .................................................................. 87 A Pest Risk Analysis on Xylella fastidiosa for the countries of the Near East Plant Protection Organization, focusing on the olive-infecting strain – Chouibani M., Fawzy A., AL Awad E., Eid P., Akarid N., Shubib S., Barham H., Smith J. ........................................................ 91 2 Options Méditerranéennes A No.121 Pest risk analysis on Xylella fastidiosa in Palestine – Shubib S., Hamdan I. ....................................... 93 Work done and actions taken on Xylella fastidiosa in Lebanon – Choueiri E. ................................ 97 Regulations enforced against Xylella fastidiosa in Jordan – Meihiar M., Setan S. ............................ 101 Regulatory