Recombinant Human IL12 Protein,HEK293 Expressed Catalog # AMS.IL2-H4210 For Research and Further Cell Culture Manufacturing Use Description Source Recombinant Human IL12 Protein,HEK293 Expressed (Human IL12B & IL12A heterodimer) Ile 23 - Ser 328 (Accession # (AAH67499,IL12B) and Arg 23 - Ser 219 (P29459,IL12A)) was produced in human 293 cells (HEK293) Predicted N‐terminus Ile 23 (IL12B) and Arg 23 (IL12A) Molecular Human IL12B & IL12A heterodimer contains no "tag", and has a calculated MW of 36.6 kDa (IL12B) and 23.8 kDa (IL12A). The Characterization predicted N-terminus is Ile 23 (IL12B) and Arg 23 (IL12A). DTT-reduced Protein migrates as 33-47 kDa in SDS-PAGE due to glycosylation. Endotoxin Less than 1.0 EU per μg of the Human IL12B & IL12A heterodimer by the LAL method. Purity >95% as determined by SDS-PAGE. Bioactivity The bio-activity was determined by the dose-dependent release of IFN-gamma from the human NK92 cell line in presence of 20 ng/mL rhIL-2 (Catalog # IL2-H4216). The ED50 < 0.2 ng/mL, corresponding to a specific activity of >5,000,000 Unit/mg. Formulation and Storage Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. Contact us for customized product form or formulation. Reconstitution See Certificate of Analysis for reconstitution instructions and specific concentrations. Storage Lyophilized Protein should be stored at -20℃ or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20℃ or -70℃. Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: ● 4-8℃ for 12 months in lyophilized state; ● -70℃ for 3 months under sterile conditions after reconstitution. Background and References Background Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. References (1) Kaliński P., et al., 1997, J. Immunol. 159 (1): 28–35. (2) Hsieh CS., et al., 1993, Science 260 (5107): 547–9. (3) Wang KS., et al., 2000, Blood 95 (10): 3183–90. Recombinant Human IL12 Protein,HEK293 Expressed Catalog # AMS.E-EL-H2364 For Research and Further Cell Culture Manufacturing Use Assay Data SDS‐PAGE Data The purity of Human IL12B & IL12A heterodimer was determined by DTT- reduced (+) SDS-PAGE and staining overnight with Coomassie Blue..