NeoBiotechnologies, Inc. 2 Union Square Union City, CA 94587 Tel: 510-376-5603 Email: [email protected] , [email protected] Website: www.NeoBiotechnologies.com TGF-alpha (Transforming Growth Factor alpha) Mouse Monoclonal Antibody [Clone SPM542] Catalog No Format Size Price (USD) 7039-MSM2X-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00 7039-MSM2X-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 459.00 7039-MSM2X-P1BX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 459.00 Human Entrez Gene ID 7039 Immunogen A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα. Human SwissProt P01135 Host / Ig Isotype Mouse / IgM, kappa Human Unigene 170009 Mol. Weight of Antigen ~6kDa Human Gene Symbol TGFA Cellular Localization Cytoplasmic and Extracellular (secreted) Human Chromosome 2p13.3 Location Species Reactivity Human. Rabbit. Zebrafish. Synonyms EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Positive Control Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast Waved 1 cancer, Melanoma. Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Monoclonal Antibody (SPM542) Specificity & Comments Supplied As This antibody reacts with the TGF alpha and shows no cross-reaction with 200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM EGF and the neuropeptide synenkephalin. The staining with this MAb is PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide completely blocked by the peptide used for raising this antibody. TGF at 1.0mg/ml. (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell Storage and Stability proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. Known Applications & Suggested Dilutions Limitations This antibody is available for research use only and is not approved for use in diagnosis. Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Warranty Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining There are no warranties, expressed or implied, which extend beyond this of formalin-fixed tissues requires heating tissue sections in 10mM Tris with description. Company is not liable for any personal injury or economic 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 loss resulting from this product. minutes) Optimal dilution for a specific application should be determined. Key References 1. Nasim, M. M., Thomas, D. M., Alison, M. R., and Fil]ipe, M. I. Transforming growth factor a expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma - an immunohistochemical study, Histopathology. 20: 339-343, 1992. 2. Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35. 3. Bebok Z; Szekeres G; Horvath G; Duda E; Nemeth P. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use]. Orvosi Hetilap, 1993, 134(24):1303-7. Language: Hungarian. NBT00785.