Proprotein Convertase 2 Antibody [Discontinued, view alternative antibodies] (R31197) Catalog No. Formulation Size R31197 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request Availability Discontinued Species Reactivity Human, Mouse, Rat Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Antigen affinity Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal UniProt P16519 Applications Western blot : 0.5-1ug/ml IHC (FFPE) : 0.5-1ug/ml Limitations This Proprotein Convertase 2 antibody is available for research use only. Western blot testing of Proprotein Convertase 2 antibody and PANC cell lysate. Predicted/expected size ~71KD IHC-P: Proprotein Convertase 2 antibody testing of rat pancreas tissue Description Proprotein Convertase, Subtilisin/Kexin-Type, 2, also known as PC2 or NEC2, is an enzyme responsible for the first step in the maturation of many neuroendocrine peptides from their precursors, such as the conversion of proinsulin to insulin intermediates. Seidah et al.(1991) mapped the gene to human chromosome 20p11.2-p11.1. Ohagi et al.(1992) observed expression of the chloramphenicol acetyltransferase reporter fusions containing the putative promoter region in insulinoma cells but not in hepatoma cells, consistent with the known tissue-specific pattern of expression of the gene. Analysis of the level of CAT activity with several deletion mutants identified the region from -1100 to -539 from the translation start site as essential for PC2 promoter activity. Application Notes The stated application concentrations are suggested starting amounts. Titration of the Proprotein Convertase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity. Immunogen An amino acid sequence from the N-terminus of human Proprotein Convertase 2 (GYRDINEIDINMNDPL) was used as the immunogen for this Proprotein Convertase 2 antibody (100% homologous in human, mouse and rat). Storage After reconstitution, the Proprotein Convertase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.