Amersham™ Imager 600 Including: 600, 600 UV, 600 QC, 600 RGB Operating Instructions Original instructions Table of Contents Table of Contents 1 Introduction .......................................................................................................... 4 1.1 Important user information ............................................................................................................. 5 1.2 Regulatory information ...................................................................................................................... 7 2 Safety instructions ............................................................................................... 11 2.1 Safety precautions ............................................................................................................................... 12 2.2 Labels ......................................................................................................................................................... 22 2.3 Emergency procedure ........................................................................................................................ 25 2.4 Recycling information ......................................................................................................................... 26 2.5 Declaration of Hazardous Substances (DoHS) ........................................................................ 27 3 Instrument description ....................................................................................... 29 3.1 Introduction ............................................................................................................................................. 30 3.2 Feature overview .................................................................................................................................. 31 3.3 Illustration of the instrument ........................................................................................................... 32 3.4 Instrument hardware description ................................................................................................. 33 3.5 Instrument software description ................................................................................................... 40 3.5.1 Description of the Capture tab ................................................................................................. 41 3.5.2 Description of the Library tab ................................................................................................... 46 3.5.3 Description of the image view .................................................................................................. 48 3.5.4 Description of the analysis workflow .................................................................................... 52 3.5.5 Description of settings views .................................................................................................... 55 3.6 Accessories .............................................................................................................................................. 59 4 Installation ............................................................................................................ 62 4.1 Site requirements .................................................................................................................................. 63 4.2 Instrument setup .................................................................................................................................. 65 4.3 System settings ..................................................................................................................................... 66 4.3.1 Date and time .................................................................................................................................. 67 4.3.2 Focus ................................................................................................................................................... 69 4.3.3 Instrument indicator ..................................................................................................................... 71 4.3.4 Service ................................................................................................................................................ 72 4.4 Network ..................................................................................................................................................... 74 4.4.1 Network setup ................................................................................................................................. 75 4.4.2 Network access from instrument ............................................................................................ 79 4.4.3 Instrument access from network ............................................................................................ 81 5 Operation .............................................................................................................. 84 5.1 Operation flow chart ........................................................................................................................... 85 5.2 Turn on the instrument ...................................................................................................................... 86 5.3 Select tray ................................................................................................................................................ 89 5.4 Place the tray in the instrument .................................................................................................... 90 5.5 Capture an image ................................................................................................................................. 93 5.5.1 Available methods ......................................................................................................................... 94 5.5.2 Chemiluminescence capture ..................................................................................................... 95 2 Amersham Imager 600 Operating Instructions 29-0645-17 AD Table of Contents 5.5.3 Colorimetric capture ..................................................................................................................... 118 5.5.4 Fluorescence capture ................................................................................................................... 122 5.6 View the image ...................................................................................................................................... 144 5.7 Save and manage image files ........................................................................................................ 148 5.7.1 Save locations ................................................................................................................................. 149 5.7.2 Save images ..................................................................................................................................... 152 5.7.3 File manage options ..................................................................................................................... 157 5.8 Analyze the image ................................................................................................................................ 160 5.8.1 Lane creation ................................................................................................................................... 163 5.8.2 Background Subtraction ............................................................................................................. 165 5.8.3 Band detection ................................................................................................................................ 169 5.8.4 Molecular weight assignment .................................................................................................. 171 5.8.5 Normalization .................................................................................................................................. 174 5.8.6 Summary ........................................................................................................................................... 175 5.9 Turn off the instrument ...................................................................................................................... 177 6 Analysis software ................................................................................................. 178 6.1 Software installation and activation ............................................................................................ 179 6.1.1 Amersham Imager 600 Analysis Software installation ................................................. 180 6.1.2 Amersham Imager 600 Analysis Software registration ................................................ 183 6.2 Software operation .............................................................................................................................. 187 6.2.1 Lane creation ................................................................................................................................... 191 6.2.2 Background subtraction ............................................................................................................. 193 6.2.3 Band detection ................................................................................................................................ 198 6.2.4 Molecular weight assignment .................................................................................................