Affinity Biosciences website:www.affbiotech.com order:[email protected] TBRG4 Antibody Cat.#: DF6079 Concn.: 1mg/ml Mol.Wt.: 71kDa Size: 100ul,200ul Source: Rabbit Clonality: Polyclonal Application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 *The optimal dilutions should be determined by the end user. Reactivity: Human,Mouse,Rat Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Specificity: TBRG4 Antibody detects endogenous levels of total TBRG4. Immunogen: A synthesized peptide derived from human TBRG4, corresponding to a region within C-terminal amino acids. Uniprot: Q969Z0 Description: TBRG4 (transforming growth factor beta regulator 4), also known as CPR2 (cell cycle progression restoration protein 2) or FASTKD4 (FAST kinase domain-containing protein 4), is a 631 amino acid protein that contains one RAP domain and belongs to the FAST kinase family. TBRG4 is ubiquitously expressed and may have a role in cell cycle progression. Existing as two alternatively spliced isoforms, the gene encoding TBRG4 maps to human chromosome 7p13. Chromosome 7 is approximately 158 million bases long, encodes over 1000 genes and makes up about 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. The deletion of a portion of the q arm of chromosome 7 is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual comfort and friendliness with strangers and an elfin appearance. Deletions of portions of the q arm of chromosome 7 are also seen in a number of myeloid disorders including cases of acute myelogenous leukemia and myelodysplasia. Storage Condition and Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM Buffer: NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. 1 / 3 Affinity Biosciences website:www.affbiotech.com order:[email protected] Western blot analysis of extracts from Myeloma cells, using TBRG4 Antibody. The lane on the left was treated with blocking peptide. Western blot analysis of extracts from Hela , using TBRG4 antibody. The lane on the left was treated with the antigen- specific peptide. DF6079 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. DF6079 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody. DF6079 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody. DF6079 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(DF6079 1:200) and mouse anti- beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. 2 / 3 Affinity Biosciences website:www.affbiotech.com order:[email protected] IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. For Research Use Only. Not for use in diagnostic and therapeutic procedures. Not for resale without express authorization. 3 / 3 Powered by TCPDF (www.tcpdf.org).