5th International Meeting Stem Cell Network North Rhine-Westphalia _March 24th –25th, 2009 _Final Program _Poster Abstracts _Company Profiles _Contact _Program Tuesday, March 24th 8:00 - 9:00 am _ Registration SPP 1356 ‘Pluripotency & Reprogramming’: Reprogramming I, Chair: A. Müller 9:00 - 9:30 am _ Austin Cooney, Houston Alternative pathways to maintain pluripotency 9:30 - 10:00 am _ Theodore Rasmussen, Storrs, Connecticut Direct reprogramming of somatic cells: From ES cell fusion to iPS 10:00 - 10:30 am _ Paul Robson, Singapore Insights into blastocyst formation revealed by single cell analysis 10:30 - 11:00 am _ Miodrag Stojkovic, Valencia Potential of embryonic and adult stem cells 11:00 - 11:15 am _ Coffee Break, Poster Session 11:15 - 11:45 am Opening of the NRW-Meeting _Thomas Rachel (Parliamentary State Secretary of Education and Research) Keynote Lectures, Chair: H. Schöler 11:45 - 12:30 am _ John Gurdon, Cambridge, UK Nuclear reprogramming by eggs and oocytes 12:30 - 1:15 am _ Bartha Knoppers, Montreal Title to be announced 1:15 - 2:30 pm _ Lunch Break, Poster Session Reprogramming II, Chair: M. Zenke 2:30 - 3:00 pm _ Huck-Hui Ng, Singapore Deciphering and reconstruction of embryonic stem cell transcriptional regulatory network 3:00 - 3:30 pm _ Alexander Meissner, Cambridge, USA Dissecting the mechanism of reprogramming 3:30 - 4:00 pm _ Coffee Break, Poster Session Mechanisms Regulating the Stem Cell State, Chair: A. Faissner 4:00 - 4:30 pm _ Ian Chambers, Edinburgh Transcription factor control of ES cell self-renewal 4:30 - 4:45 pm _ Jens Schwamborn, Münster 4:45 - 5:15 pm _ Niall Dillon, London Combinatorial histone modifications and the epigenetic regulation of stem cell commitment and differentiation 5:15 - 6:45 pm Poster Session 7:00 - 10:30 pm _ Networking Event at the historic Aula Carolina (bus transport provided) Wednesday, March 25th 9:00 - 10:00 am _ Ethical Issues regarding Therapeutic Experiments (Panel Discussion) Stem Cell Differentiation, Chair: S. Schlatt 10:00 - 10:30 am _ Renee Reijo-Pera, Stanford Human germ cell differentiation from hESCs and iPSCs 10:30 - 10:45 am Kinarm Ko, Münster 10:45 - 11:15 am _ Timm Schroeder, Munich Tracking of stem cell behavior at the single cell level: New tools for old questions 11:15 - 11:45 am _ Coffee Break, Poster Session Cancer Stem Cells, Chair: T. Dittmar 11:45 - 12:15 am _ Maarten van Lohuizen, Amsterdam Polycomb repressors controlling stem cell fate: Implications for cancer and development 12:15 - 12:45 am _ Andeas Trumpp, Heidelberg Dormancy in stem cells 12:45 - 2:15 pm _ Lunch Break, Poster Session Therapeutic Devices I, Chair: E. Tobiasch 2:15 - 2:45 pm _ Benjamin E. Reubinoff, Jerusalem Human embryonic stem cell – towards future therapy of neurological disorder 2:45 -3:00 pm _ Sabine Neuss, Aachen 3:00 - 3:30 pm _ Mathis Riehle, Glasgow Engineering cell function and differentiation in 2 and 3 dimensions with mirco- and nanotopographies 3:30 - 4:00 pm _ Coffee Break Therapeutic Devices II, Chair: O. Brüstle 4:00 - 4:30 pm _ Christine Mummery, Utrecht Cardiomyocytes from human embryonic stem cells 4:30 - 4:45 pm _ Ruth Olmer, Hannover 4:45 - 5:15 pm _ Markus Grompe, Portland Hepatic stem cells and their medical use following Poster Awarding and Conclusion _Register Bioengineering 1 _ Simone Haupt Automated selection and harvesting of pluripotent stem cell colonies using the CellCelector ................................. 17 2 _ Mareike Hoss The Impact of Adhesion Molecules on Stem Cell/Biomaterial Interactions ................................................................ 18 3 _ Sebastian Schwarz Influence of engineered magnetic nanoparticles on hematopoietic stem cells ..........................................................19 Cancer & Stem Cells 4 _ Verena Börger Identification of Cancer Stem Cell-Like Cells and Stromal Cells in Renal Cell Carcinoma ......................................... 21 5 _ Anne Fassl Apoptosis resistance of glioblastoma stem cells – a question of Notch? ....................................................................22 6 _ Martin Götte siRNA-mediated targeting of the adult progenitor cell marker Musashi-1 in endometrial carcinoma leads to a modulation of p21WAF-dependent cell cycle progression and apoptosis via downregulation of the notch-1/Hes1-pathway ...........................................................................................................................................23 7 _ Ulf Kahlert The influence of in vitro conditions for the enrichment of stem-like cell population in primary human brain tumor cultures: hypoxia- mediated upregulation of Sonic Hedgehoc (SHH) -pathway .......................24 8 _ Christa Nagler Characterization of breast cancer cell and BMDC derived hybrid cells ...................................................................... 26 9 _ Daniel Nettersheim The seminoma-like cell line TCam-2 gains differentiation potential upon FGF4 treatment independent of a seminoma to embryonal carcinoma transition ................................................................................ 28 10 _ Georg Reith Influence of cytokines and chemokines on breast cancer cells, breast stem cells and their hybrid cells ................ 29 11 _ Vida Vafaizadeh Genetically modified mammary stem cells define distinct roles for Stat5 in mammary gland development and breast cancer ...................................................................................................................................... 31 12 _ Christian G. Ziegler Dehydroepiandrosterone (DHEA) and its sulphate ester (DHEAS) exert a prominent effect on chromaffin PC12 cell differentiation ................................................................................................................................32 Chromatin and Epigenetics 13 _ Anita Sabine Bledau Characterization of the early embryo upon loss of histone methyltransferase Setd1a ............................................. 34 14 _ Peter Dröge HMG proteins: the new guardians of embryonic stem cell genomes ...........................................................................35 15 _ Khursheed Iqbal Mammalian embryos respond to 5-cytosine methylation at CpG or GpC dinucleotides .......................................... 36 16 _ Andrea Kranz Fetal and adult hematopoiesis requires continuous Mll1 function ...............................................................................37 17 _ Nadine Obier Analyzing epigentic modifications in pluripotent stem cells ........................................................................................ 38 18 _ Simeon Santourlidis Incomplete epigenetic repression of embryonic stem cell pluripotency genes in unrestricted somatic stem cells (USSC) from human umbilical cord blood .................................................................................... 39 19 _ Ullrich Zechner Comparative methylation profiles and telomerase biology of mouse multipotent adult germline stem cells and embryonic stem cells ............................................................................................................................. 40 Development and Regeneration 20 _ Lodovica Borghese Inhibition of Notch signaling in human ES cell-derived neural stem cells accelerates their differentiation into functional neurons .......................................................................................................................... 42 21 _ Carmen Carillo-García Growth/differentiation factor (GDF) 15 regulates cell cycle exit of secondary progenitors in the developing mouse ganglionic eminence ........................................................................................................................ 43 22 _ Koushik Chakrabarty Characterization of Novel Nurr1 Splicing Variant During Developent of Dopaminergic Neurons ............................. 44 23 _ Xiaolei Ding BMI1 Transcriptional Repressor Promotes Hematopoietic Cell Development from ES Cells .................................... 45 24 _ Daniela Dreymüller Molecular and functional characterization of macrophage subtypes involved in cell-material interactions and wound healing ...................................................................................................................................... 46 25 _ Lukas P. Frenzel Role of NKG2D-ligands and ICAM-1 in NK cell-mediated lysis of murine embryonic stem cells and embryonic stem cell-derived cardiomyocytes ........................................................................................................47 26 _ Tobias Führmann An in vitro investigation of the effects of multipotent progenitors and their astroglial derivates on DRG axonal growth ..................................................................................................................................................... 48 27 _ Wiebke Garrels Experimental modulation of telomerase activity in bovine embryos .......................................................................... 49 28 _ Luca Gentile Essential role for Smed-LSm protein for planarian stem cell proliferation ................................................................. 50 29 _ Marcus Jäger Is Bone Marrow Concentrate Suitable to Treat Local Bone Defects? ........................................................................... 51 30 _ Justyna Jozefczuk Differentiation of Human Embryonic Stem Cells into Hepatocytes-like cells ..............................................................53 31 _ Michael Karus Regulation of