10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 USP10 Antibody (Center) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP17199c Specification USP10 Antibody (Center) - Product Information Application WB,E Primary Accession Q14694 Other Accession Q3KR59, P52479, Q5ZJN4, A5PJS6, Q7ZXR7, Q2NL57, NP_005144.2 Reactivity Human Predicted Xenopus, Bovine, Chicken, Mouse, Rat Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Calculated MW 87134 USP10 Antibody (Center) (Cat. #AP17199c) Antigen Region 500-529 western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the USP10 Antibody (Center) - Additional Information USP10 antibody detected the USP10 protein (arrow). Gene ID 9100 USP10 Antibody (Center) - Background Other Names Ubiquitin carboxyl-terminal hydrolase 10, Ubiquitin is a highly conserved protein that is Deubiquitinating enzyme 10, Ubiquitin covalently thioesterase 10, Ubiquitin-specific-processing protease 10, linked to other proteins to regulate their USP10, KIAA0190 function and degradation. This gene encodes a member of Target/Specificity the ubiquitin-specific This USP10 antibody is generated from protease family of cysteine proteases. The rabbits immunized with a KLH conjugated enzyme specifically synthetic peptide between 500-529 amino cleaves ubiquitin from ubiquitin-conjugated acids from the Central region of human protein substrates. The USP10. protein is found in the nucleus and cytoplasm. It functions as a Dilution co-factor of the DNA-bound androgen receptor WB~~1:1000 complex, and is inhibited by a protein in the Ras-GTPase Format pathway. The human genome Purified polyclonal antibody supplied in PBS contains several pseudogenes similar to this with 0.09% (W/V) sodium azide. This gene. [provided by antibody is purified through a protein A RefSeq]. column, followed by peptide affinity purification. USP10 Antibody (Center) - References Storage Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Maintain refrigerated at 2-8°C for up to 2 (2010) : weeks. For long term storage store at -20°C Yuan, J., et al. Cell 140(3):384-396(2010) in small aliquots to prevent freeze-thaw Bomberger, J.M., et al. J. Biol. Chem. cycles. 284(28):18778-18789(2009) Gudbjartsson, D.F., et al. Nat. Genet. Precautions 40(5):609-615(2008) USP10 Antibody (Center) is for research use Olsen, J.V., et al. Cell 127(3):635-648(2006) only and not for use in diagnostic or therapeutic procedures. USP10 Antibody (Center) - Protein Information Name USP10 Synonyms KIAA0190 Function Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:<a href="http://www.uniprot.org/c itations/25861989" target="_blank">25861989</a>). Deubiquitinates TBX21 leading to its stabilization (PubMed:<a href="http://www. uniprot.org/citations/24845384" target="_blank">24845384</a>). Cellular Location Page 2/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions (PubMed:20096447). After DNA damage, translocates to the nucleus following phosphorylation by ATM (PubMed:20096447) Tissue Location Widely expressed.. USP10 Antibody (Center) - Protocols Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture Page 3/3 Powered by TCPDF (www.tcpdf.org).